TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium transferase [Homo sapiens] (501 letters) Database: C.owczarzaki/genome.fa 89 sequences; 28,043,798 total letters Searching........................................................................................done Score E Sequences producing significant alignments: (bits) Value supercontig_1.13 of Capsaspora owczarzaki ATCC 30864 157 1e-78 supercontig_1.4 of Capsaspora owczarzaki ATCC 30864 29 8.4 >supercontig_1.13 of Capsaspora owczarzaki ATCC 30864 Length = 955311 Score = 157 bits (396), Expect(2) = 1e-78 Identities = 85/192 (44%), Positives = 110/192 (57%), Gaps = 47/192 (24%) Frame = +2 Query: 90 RFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAG------------------- 130 R HGIGRSGDI+A+QPKAAGSSL+ K+ N + LD+I+LAG Sbjct: 800303 RMAHGIGRSGDIAAIQPKAAGSSLIMKLVNIMCLDVIRLAGMILCHTVWQHEDQSEIRRK 800482 Query: 131 ---------------------------VHTVANCFVVPMATGMSLTLCFLTLRHKRP-KA 162 VH C +P+ATGM++ + LTL RP A Sbjct: 800483 WTSFLTTSQQPTHNLKQLTIQ*SHWTGVHAANACIALPLATGMAMVMTLLTLAQTRPPSA 800662 Query: 163 KYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCILCIHS 222 + ++W RIDQKSC K++ GFE VV++N +EGDE+RTDL A++ +V +G D ILCI S Sbjct: 800663 RKVLWLRIDQKSCIKAITACGFEVVVVDNAIEGDEVRTDLSALQHQVNTVGKDNILCIVS 800842 Query: 223 TTSCFAPRVPDR 234 TTSCFAPR PDR Sbjct: 800843 TTSCFAPRCPDR 800878 Score = 155 bits (392), Expect(2) = 1e-78 Identities = 112/310 (36%), Positives = 148/310 (47%), Gaps = 84/310 (27%) Frame = +3 Query: 237 ELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIA 296 E+A ICA IPH++NNAYG+QSSK LIQ + GR+DAFVQS DKN MVPVGGA++A Sbjct: 800979 EIAKICATESIPHVINNAYGIQSSKITALIQDAHKAGRVDAFVQSSDKNLMVPVGGALVA 801158 Query: 297 GFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMF----------- 345 G + +FI+ I +MYPGRASA+PS+D+ ITLLS+G G++ LLK+RK + Sbjct: 801159 GHDRAFIKRIGEMYPGRASAAPSVDIFITLLSMGQQGFRSLLKQRKVTWILALEFCGFIL 801338 Query: 346 --------------------SYLSNQIKKLSEAYNE--RLLHTPHN-----PISLAMTLK 378 S+ N + S N + L P + P +A TL+ Sbjct: 801339 HTSAQLIYEC*IVLYRLCLNSFEQNSARLPSAKVNGCWKPLTIPFHSV*FKPPLVA*TLR 801518 Query: 379 TLDEH---RDKAV---------TQLGSMLFTRQVSGARVV-------------------- 406 H R A+ T+LGS LFTR VSG R Sbjct: 801519 CKSPHLCFRPIAITLSTFGSDATRLGSFLFTRCVSGTRCAVALAPGIVLASCL*FSFTHF 801698 Query: 407 --------------PLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIK 452 + +V + F +H + Y AYL AA+AIGM Q+VD I Sbjct: 801699 HLEFCIYCSVVAADRAAATTSVGPVSLSNFGAHHSAYHGAYLTAAAAIGMTEQEVDALID 801878 Query: 453 RLDRCLKAVR 462 RL K +R Sbjct: 801879 RLTAAFKDLR 801908 Score = 42.4 bits (98), Expect(2) = 5e-09 Identities = 20/34 (58%), Positives = 24/34 (70%) Frame = +1 Query: 35 LLEKGKCPENGWDESTLELFLHELAIMDSNNFLG 68 L + K PE GWDE ++EL L +LA MDSNNF G Sbjct: 799918 LSRQRKWPELGWDEMSIELLLSDLAQMDSNNFPG 800019 Score = 36.6 bits (83), Expect(2) = 5e-09 Identities = 17/24 (70%), Positives = 18/24 (75%) Frame = +3 Query: 67 LGNCGVGEREGRVASALVARRHYR 90 L N G GERE RV S LVARRH+R Sbjct: 800091 LANIGAGEREARVYSDLVARRHFR 800162 >supercontig_1.4 of Capsaspora owczarzaki ATCC 30864 Length = 2141673 Score = 28.9 bits (63), Expect = 8.4 Identities = 11/31 (35%), Positives = 20/31 (64%) Frame = +3 Query: 339 KERKEMFSYLSNQIKKLSEAYNERLLHTPHN 369 K++K+ + + Q KK ++ YN RL+ PH+ Sbjct: 454998 KKKKKKKTSMQTQYKKTNKTYNLRLMRVPHS 455090 Database: C.owczarzaki/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 28,043,798 Number of sequences in database: 89 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,111,948 Number of Sequences: 89 Number of extensions: 248363 Number of successful extensions: 1496 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 1291 Number of HSP's gapped (non-prelim): 465 length of query: 501 length of database: 9,347,932 effective HSP length: 104 effective length of query: 397 effective length of database: 9,338,676 effective search space: 3707454372 effective search space used: 3707454372 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 62 (28.5 bits)