TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|267844904|ref|NP_058651.3| O-phosphoseryl-tRNA(Sec) selenium transferase [Homo sapiens] (501 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-t... 346 2e-95 emb|FN668653.1| Blastocystis hominis, Singapore isolate B (sub-t... 33 0.23 emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 1.9 emb|FN668649.1| Blastocystis hominis, Singapore isolate B (sub-t... 29 5.7 emb|FN668643.1| Blastocystis hominis, Singapore isolate B (sub-t... 28 9.6 >emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_1 Length = 1866585 Score = 346 bits (887), Expect = 2e-95 Identities = 196/515 (38%), Positives = 299/515 (58%), Gaps = 47/515 (9%) Frame = -2 Query: 1 MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEKGKCPENGWDESTLE---LFLHE 57 MN ++F L+ YV QG +A + E+L++ LL K PE GWD+ ++E + L+ Sbjct: 1211609 MNEKNFEYARSLIPATYVNQGRDAIKRRENLVKDLLTHRKIPEIGWDDQSIE*KTIPLYS 1211430 Query: 58 LAIMDSNNFLG-----NCGVGEREGRVASALVARRHYRFIHGIG---------------- 96 FL + G+ E V L A +Y I + Sbjct: 1211429 RLDFS*TTFLSWTAITSWGIAESVS-VKGELRALWYYAMILCLSGCQASFLSCSWNWESG 1211253 Query: 97 -------RSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVANCFVVPMATGMSLT 149 ++ D + PKAAGSSL+ ++T + +D I+ G+ V +C V+P+ATGMS++ Sbjct: 1211252 *CGCNPSKT*DHVEL*PKAAGSSLICELTRKMAVDFIQTCGITEVKDCIVLPLATGMSIS 1211073 Query: 150 LCFLTLRHKRPKAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKV 209 +C L+LR P AKY+IW R+DQK+C KS++T G EP++I+ V EGDEL T+L A+ K+ Sbjct: 1211072 MCLLSLRLNNPTAKYVIWFRVDQKTCLKSILTTGLEPIIIDPVPEGDELNTNLGALREKL 1210893 Query: 210 QELGPDCILCIHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQG 269 + + + ILCI TTS FAPR+PD + ++ + + +P IVNNAYG+QS I + Sbjct: 1210892 ESIKREEILCIIPTTSVFAPRLPDDIVGVSKLAKEFSVPVIVNNAYGLQSGATCKAIDRA 1210713 Query: 270 ARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSL 329 RVGRID V S DKN +VPVGGA++ + + K YPGRAS SP LDV ITLLS+ Sbjct: 1210712 IRVGRIDMLVSSTDKNMLVPVGGALVYSPQSGMVDLVRKNYPGRASMSPILDVFITLLSM 1210533 Query: 330 GSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKT------LDEH 383 G + +LKER++ + YL +++ +++ Y +R+L TP NPIS+A+TL E+ Sbjct: 1210532 GKRELRSVLKEREDCYQYLLDRLGEVAGRYKQRVLRTPRNPISIAVTLDNPFPEDDSFEY 1210353 Query: 384 RDKAVTQLGSMLFTRQVS----------GARVVPLGSMQTVSGYTFRGFMSHTNNYPCAY 433 D+ +T+ G+ML+TR VS G+R V + ++V Y F + HT++YP AY Sbjct: 1210352 SDEDITKFGAMLYTRGVSGRVVEIV*SIGSRCVGFHNKKSVGKYEFANYGGHTDHYPHAY 1210173 Query: 434 LNAASAIGMKMQDVDLFIKRLDRCLKAVRKERSKE 468 +A+AIG+K +++D+FI RL++ K +K+ K+ Sbjct: 1210172 FTSAAAIGVKKEEIDVFIDRLEKNWKQYQKDLEKK 1210068 >emb|FN668653.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_22 Length = 243064 Score = 33.5 bits (75), Expect = 0.23 Identities = 29/114 (25%), Positives = 47/114 (41%), Gaps = 5/114 (4%) Frame = -3 Query: 334 YKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGS 393 ++ L+ RKE + + + LS Y PHNPI L + L LD H AV G+ Sbjct: 196202 FQSTLQNRKEFKLFRQSMVVWLSRVYQ------PHNPICLTLLLYLLDTH---AVMNSGT 196050 Query: 394 MLFTRQVSGARVVPLGSMQTVS-----GYTFRGFMSHTNNYPCAYLNAASAIGM 442 + Q + + + V+ GY F G S + C ++ + I + Sbjct: 196049 ISNDIQNDLTKFIHSIDLSEVNLNDFMGYLFTGVTSSDSVVCCFFVQHSFVISL 195888 >emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_5 Length = 953317 Score = 30.4 bits (67), Expect = 1.9 Identities = 21/77 (27%), Positives = 36/77 (46%) Frame = +3 Query: 334 YKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLGS 393 +KKLLKE+K++ + K+ ++ +R TPHN + A K + +D V + Sbjct: 341817 FKKLLKEKKKLKKQKKREKKQRNK---QRCKSTPHNQTNAANASKQTNVPKDSNVPNASN 341987 Query: 394 MLFTRQVSGARVVPLGS 410 + S VP+ S Sbjct: 341988 VPNVPNTSNEPNVPIVS 342038 >emb|FN668649.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_19 Length = 315857 Score = 28.9 bits (63), Expect = 5.7 Identities = 13/33 (39%), Positives = 21/33 (63%) Frame = +2 Query: 465 RSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQ 497 R KE NY KTE DI+ ++L+++L D ++ Sbjct: 221843 RLKEEFANYQKTEKEDIKNRKVELESMLSDIHE 221941 >emb|FN668643.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_13 Length = 486799 Score = 28.1 bits (61), Expect = 9.6 Identities = 19/52 (36%), Positives = 28/52 (53%) Frame = -2 Query: 327 LSLGSNGYKKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLK 378 L + S+G+KKLLKER M + + K LS + + L P SL + L+ Sbjct: 309027 LQVESDGFKKLLKERDRMIHLQTWKTKSLS-SLSRSLPGLPFPRTSLLLRLR 308875 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.320 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,261,592 Number of Sequences: 54 Number of extensions: 151692 Number of successful extensions: 942 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 861 Number of HSP's gapped (non-prelim): 189 length of query: 501 length of database: 6,272,415 effective HSP length: 101 effective length of query: 400 effective length of database: 6,266,961 effective search space: 2506784400 effective search space used: 2506784400 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 61 (28.1 bits)