TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74761781|sp|Q9NX07.1|TSAP1_HUMAN RecName: Full=tRNA selenocysteine 1-associated protein 1; AltName: Full=SECp43; AltName: Full=tRNA selenocysteine-associated protein 1 (287 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000630.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 64 1e-10 gb|CM000609.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 40 0.002 gb|CM000606.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 39 0.003 gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 36 0.021 gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 34 0.081 gb|CM000607.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 33 0.14 gb|CM000612.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 32 0.52 gb|CM000631.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 30 1.5 gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 30 1.5 gb|CM000621.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 28 5.8 >gb|CM000630.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 28, whole genome shotgun sequence Length = 387582 Score = 63.9 bits (154), Expect = 1e-10 Identities = 44/157 (28%), Positives = 81/157 (51%), Gaps = 1/157 (0%) Frame = -1 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63 SL++GDL P ++E+ + F+ +G V S+++ R+ +T GY +V F +A AE+ + Sbjct: 302808 SLYVGDLAPDVNESLLFEIFSAVGP-VASIRVCRDAVTRRSLGYSYVNFHQMADAERAMD 302632 Query: 64 KINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSC 123 +N + G K ++ ++ S ++FV +L +D+ LY+ F ++ + Sbjct: 302631 TMNFSMIKG----KPCRIMWSQRDPSLRRSGVGNIFVKNLNEAIDNKQLYDTF-SLFGNI 302467 Query: 124 RGGKVVLDQTG-VSKGYGFVKFTDELEQKRALTECQG 159 KVV D+ G VS GYG+V + A+ + G Sbjct: 302466 LSCKVVTDREGGVSMGYGYVHYETAEAANAAIEKLDG 302356 Score = 40.8 bits (94), Expect = 9e-04 Identities = 30/88 (34%), Positives = 46/88 (52%), Gaps = 1/88 (1%) Frame = -1 Query: 84 ATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFV 142 +T P +P SL+VGDL PDV++ +L+E F V P +V D T S GY +V Sbjct: 302841 STASDNPFQTP--SLYVGDLAPDVNESLLFEIFSAVGP-VASIRVCRDAVTRRSLGYSYV 302671 Query: 143 KFTDELEQKRALTECQGAVGLGSKPVRL 170 F + +RA+ ++ + KP R+ Sbjct: 302670 NFHQMADAERAMDTMNFSM-IKGKPCRI 302590 >gb|CM000609.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 6, whole genome shotgun sequence Length = 1035082 Score = 39.7 bits (91), Expect = 0.002 Identities = 45/181 (24%), Positives = 75/181 (41%), Gaps = 3/181 (1%) Frame = -2 Query: 2 AASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKC 61 A L++G++ + + + GE V+ V I + TG G+ FV + A+ Sbjct: 875913 AQKLYVGNIPFEATKEALMEFYRAYGE-VIEVYIPLDAATGNGRGFAFVTMKE-EDAQAA 875740 Query: 62 LHKINGKPLPGATPAKRFKL---NYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVK 118 + NG G L A + P N+ +++G+L+ L E F + Sbjct: 875739 IDATNGMEYEGRKMVVSVPLPPGKKAAGKRGPRNADRTKMYIGNLSFYTVPETLQELFEE 875560 Query: 119 VYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKAS 178 +TG ++G+GFV E Q RA+ E G L + +R++ A PK S Sbjct: 875559 FGTVIDCYMPEDPETGSARGFGFVTMDKEAAQ-RAIEEIDGC-ELDGRIIRVNEAQPKGS 875386 Query: 179 R 179 R Sbjct: 875385 R 875383 Score = 37.0 bits (84), Expect = 0.012 Identities = 37/150 (24%), Positives = 64/150 (42%), Gaps = 3/150 (2%) Frame = -2 Query: 33 VKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGKPLPGATPAKRFKLNYATYGKQP-- 90 V I RN++TG P G+ FV+ E + ++ + + G L K+ Sbjct: 876105 VSIPRNKMTGQPRGFAFVDMGSPEEMEAAVAGLDNQQVNGRMLRVNKSLPKEDVPKKERR 875926 Query: 91 DNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELE 149 + L+VG++ + L EF+ + Y + LD TG +G+ FV +E + Sbjct: 875925 EQDGAQKLYVGNIPFEATKEALMEFY-RAYGEVIEVYIPLDAATGNGRGFAFVTMKEE-D 875752 Query: 150 QKRALTECQGAVGLGSKPVRLSVAIPKASR 179 + A+ G G K V +SV +P + Sbjct: 875751 AQAAIDATNGMEYEGRKMV-VSVPLPPGKK 875665 Score = 32.7 bits (73), Expect = 0.23 Identities = 17/58 (29%), Positives = 30/58 (51%) Frame = +3 Query: 86 YGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVK 143 +GK+ N P L++G L P+ ++ +++F + +TG KGYGFV+ Sbjct: 935274 HGKKGRNKPGTVLYLGHLPPNFEEQEIWKFLQQFGKVLHVRLARSKKTGGGKGYGFVE 935447 >gb|CM000606.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 2, whole genome shotgun sequence Length = 1497954 Score = 38.9 bits (89), Expect = 0.003 Identities = 27/103 (26%), Positives = 50/103 (48%) Frame = +2 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 +++G+L E + + F+ +G V+ V+++ + TG P G+ FVEF+D A + Sbjct: 636419 VFVGNLAFNTTEEQLQQVFSDIGR-VIHVRLVTDVDTGKPRGFAFVEFSDAQAALSAIRN 636595 Query: 65 INGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDV 107 +N L G +R ++N++ NS G L D+ Sbjct: 636596 MNDYELNG----RRIRVNFS-------NSSHLEALAGQLGMDL 636691 Score = 29.3 bits (64), Expect = 2.6 Identities = 19/64 (29%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +1 Query: 97 SLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALT 155 +LF+G L +V + L P + +++ D + G S+GY FV++ E + KRA Sbjct: 1427494 TLFIGRLAYEVTERKLLREMESFGP-VKDIRIIKDSKEGKSRGYAFVEYEHEEDMKRAYR 1427670 Query: 156 ECQG 159 G Sbjct: 1427671 AADG 1427682 >gb|CM000605.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 1, whole genome shotgun sequence Length = 2535400 Score = 36.2 bits (82), Expect = 0.021 Identities = 35/132 (26%), Positives = 58/132 (43%), Gaps = 1/132 (0%) Frame = +3 Query: 14 MDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGK-PLPG 72 M E + F + G + V IIR++ TG+ G FV + A AE+ ++ PG Sbjct: 1255914 MSEEDVFPTFDSFGP-LKDVAIIRDKHTGLHRGCAFVTYWSAADAERAQEALHDTFTFPG 1256090 Query: 73 ATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ 132 A A + K + PE LFVG L+ + + E F + + R ++ + Sbjct: 1256091 ARRAAQVK-------PAEPSVPENKLFVGMLSRKATEVEIRELF-EPFGEIREVYMIRNA 1256246 Query: 133 TGVSKGYGFVKF 144 G SK F+++ Sbjct: 1256247 DGSSKCAAFLRY 1256282 Score = 27.3 bits (59), Expect = 9.9 Identities = 13/42 (30%), Positives = 20/42 (47%) Frame = -3 Query: 69 PLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDG 110 P+PG + + Y +QP S + S+ VG P + DG Sbjct: 2014271 PVPGRSRSGGTPRGCLVYSEQPSGSGKRSIAVGKYRPRIGDG 2014146 >gb|CM000608.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 5, whole genome shotgun sequence Length = 1098047 Score = 34.3 bits (77), Expect = 0.081 Identities = 38/171 (22%), Positives = 73/171 (42%), Gaps = 26/171 (15%) Frame = -3 Query: 2 AASLWMGDLEPYMDENFIS----RAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLAT 57 A L++G+L P++ E+ I RA T +S + + +CFVEF + Sbjct: 479403 ARRLYVGNLPPHITEDAIHVEFRRAIEIASPTPLSEDPVLSTYINHERRFCFVEFKTVEM 479224 Query: 58 AEKCLH----KINGKPL-------------PGATPAKRFKLNYATYG---KQPDNSPEYS 97 A C++ + G P+ P P+ L+ + G ++ P Sbjct: 479223 ATACMNLDGLHVQGVPVKVKRPNDYNANMAPKIHPSALPPLDVSKLGIVSGTVEDGPN-K 479047 Query: 98 LFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD--QTGVSKGYGFVKFTD 146 +F+G L + D + E ++ + + +V + ++ +SKGY FV++ D Sbjct: 479046 IFIGGLHYHLQDSQVME-LLQAFGKIKAFHLVSNDPESNMSKGYCFVEYAD 478897 Score = 27.7 bits (60), Expect = 7.6 Identities = 13/63 (20%), Positives = 29/63 (46%) Frame = -3 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 +++G L ++ ++ + G+ + + + + GYCFVE+AD + Sbjct: 479046 IFIGGLHYHLQDSQVMELLQAFGKIKAFHLVSNDPESNMSKGYCFVEYADPNITPIAVQG 478867 Query: 65 ING 67 +NG Sbjct: 478866 LNG 478858 >gb|CM000607.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 4, whole genome shotgun sequence Length = 1360148 Score = 33.5 bits (75), Expect = 0.14 Identities = 19/43 (44%), Positives = 27/43 (62%), Gaps = 1/43 (2%) Frame = +2 Query: 127 KVVLD-QTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPV 168 K+V D +TG SKG+GF FT+ + + +C GA L S+PV Sbjct: 33389 KIVRDWKTGKSKGFGFAVFTEAIYATVCIEKCHGA-KLESRPV 33514 Score = 30.0 bits (66), Expect = 1.5 Identities = 19/62 (30%), Positives = 30/62 (48%) Frame = -2 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 L++ L P + + F+ TV V+IIR++ TG Y F EF + A + K Sbjct: 512455 LFICKLNPITQDEDLELIFSRFDPTV-KVEIIRDQSTGKSLQYAFAEFEEKQQAVEAYFK 512279 Query: 65 IN 66 +N Sbjct: 512278 MN 512273 Score = 28.5 bits (62), Expect = 4.4 Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 4/79 (5%) Frame = -2 Query: 87 GKQPD---NSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFV 142 G PD +PE LF+ L P D L F + P+ + +++ DQ TG S Y F Sbjct: 512497 GDLPDADIKAPENVLFICKLNPITQDEDLELIFSRFDPTVKV-EIIRDQSTGKSLQYAFA 512321 Query: 143 KFTDELEQKRALTECQGAV 161 +F ++ + A + A+ Sbjct: 512320 EFEEKQQAVEAYFKMNNAL 512264 >gb|CM000612.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 9, whole genome shotgun sequence Length = 1002813 Score = 31.6 bits (70), Expect = 0.52 Identities = 22/70 (31%), Positives = 36/70 (51%), Gaps = 5/70 (7%) Frame = +2 Query: 97 SLFVGDLTPDVDDGMLYEFFVKV----YPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQK 151 S+FVG+L D+ L E F + +G ++V D +T KG+G+V F+D+ Sbjct: 402095 SIFVGNLPYTADEDSLAEHFCEGCGLNVDDIQGVRIVRDKETFQCKGFGYVLFSDQSMVT 402274 Query: 152 RALTECQGAV 161 AL G++ Sbjct: 402275 LALQRMSGSL 402304 >gb|CM000631.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 29, whole genome shotgun sequence Length = 384256 Score = 30.0 bits (66), Expect = 1.5 Identities = 23/78 (29%), Positives = 41/78 (52%), Gaps = 3/78 (3%) Frame = -1 Query: 97 SLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ---TGVSKGYGFVKFTDELEQKRA 153 +++VG+L + +L E F +V R V + + +G GYGFV++ D ++ A Sbjct: 259291 TVYVGNLDQACSEELLTELFSQVG---RVASVYMPKDKLSGQHNGYGFVEYLDAVDADYA 259121 Query: 154 LTECQGAVGLGSKPVRLS 171 +T + L +PVR+S Sbjct: 259120 MTILH-MMKLFGRPVRVS 259070 >gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 8, whole genome shotgun sequence Length = 1007773 Score = 30.0 bits (66), Expect = 1.5 Identities = 25/74 (33%), Positives = 34/74 (45%), Gaps = 5/74 (6%) Frame = -1 Query: 90 PDNSPEYSLFVGDLTPDVDDGMLYEFFVKV-----YPSCRGGKVVLDQTGVSKGYGFVKF 144 PD+ E +LFVG++ V D L F V P+C VV S YGFV F Sbjct: 230971 PDSMVETTLFVGNIDEFVHDDDLSGLFQSVSKLQSVPAC----VVRKPDMSSLQYGFVSF 230804 Query: 145 TDELEQKRALTECQ 158 +E+K +C+ Sbjct: 230803 -PSVEEKEVGLDCE 230765 Score = 28.9 bits (63), Expect = 3.4 Identities = 16/35 (45%), Positives = 19/35 (54%) Frame = -3 Query: 150 QKRALTECQGAVGLGSKPVRLSVAIPKASRVKPVE 184 QK T G VGLG+KP + S IP RV+ E Sbjct: 959048 QKGNSTHDSGRVGLGNKPAKASNLIPCRWRVESTE 958944 >gb|CM000621.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 19, whole genome shotgun sequence Length = 690427 Score = 28.1 bits (61), Expect = 5.8 Identities = 18/75 (24%), Positives = 34/75 (45%) Frame = +3 Query: 3 ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62 AS+W+G P ++ NFI+ + I+++ L + A + TA K Sbjct: 343362 ASVWVGTRHPVLNRNFIAASSLR--------SIVKSELN--------TDCASMCTAGKYS 343493 Query: 63 HKINGKPLPGATPAK 77 + + P+P TP++ Sbjct: 343494 SESSWGPMPSTTPSQ 343538 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.317 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,574,016 Number of Sequences: 31 Number of extensions: 82419 Number of successful extensions: 339 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 285 Number of HSP's gapped (non-prelim): 124 length of query: 287 length of database: 7,911,228 effective HSP length: 97 effective length of query: 190 effective length of database: 7,908,221 effective search space: 1502561990 effective search space used: 1502561990 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 59 (27.3 bits)