TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74761781|sp|Q9NX07.1|TSAP1_HUMAN RecName: Full=tRNA selenocysteine 1-associated protein 1; AltName: Full=SECp43; AltName: Full=tRNA selenocysteine-associated protein 1 (287 letters) Database: N.gruberi/genome.fa 784 sequences; 40,964,085 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 144 9e-35 gb|GG738891.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 56 3e-08 gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 55 1e-07 gb|GG738960.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 45 6e-05 gb|GG738879.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 41 0.001 gb|GG738928.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 38 0.009 gb|GG738866.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 36 0.047 gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 35 0.079 gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 33 0.39 gb|GG738851.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 32 0.52 gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 32 0.67 gb|GG738877.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 31 1.1 gb|GG738889.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 30 3.3 gb|GG738856.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 28 9.7 >gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome shotgun sequence Length = 707703 Score = 144 bits (363), Expect = 9e-35 Identities = 85/189 (44%), Positives = 117/189 (61%), Gaps = 17/189 (8%) Frame = -1 Query: 2 AASLWMGDLEPYMDENFISRAFAT-MGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEK 60 A +LWMGDL+ MD+ ++ + FA + ++ SVKII++R TG GY FVEFA + A+ Sbjct: 463674 ARTLWMGDLDDSMDDEYVKKLFANDIRPSINSVKIIKDRNTGKSIGYGFVEFASIEIAKA 463495 Query: 61 CLHKINGKPLPGATPAKRFKLNYAT--------YGKQPDNSPE-------YSLFVGDLTP 105 L GKP+P P K ++LN+A + QP P S+FVGDL P Sbjct: 463494 VLESYAGKPIP-TLPNKIYRLNWAAQNQGSNPLFSSQPGGKPSSGGKENIVSIFVGDLAP 463318 Query: 106 DVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALTECQGAVGLG 164 DV+D ML + F YPS RG KVV+D ++G+SKGYGFVKF DE + R++TE QG V + Sbjct: 463317 DVNDYMLEQTFRNRYPSVRGAKVVMDPKSGISKGYGFVKFADEDDMMRSMTEMQG-VYIS 463141 Query: 165 SKPVRLSVA 173 S+PV++S A Sbjct: 463140 SRPVKISHA 463114 Score = 46.6 bits (109), Expect = 3e-05 Identities = 47/212 (22%), Positives = 87/212 (41%), Gaps = 44/212 (20%) Frame = -1 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63 S+++GDL P +++ + + F +V K++ + +GI GY FV+FAD + + Sbjct: 463344 SIFVGDLAPDVNDYMLEQTFRNRYPSVRGAKVVMDPKSGISKGYGFVKFADEDDMMRSMT 463165 Query: 64 KING-----KPLPGATPAKRFK------------LNYATYGKQ----------PDNSPE- 95 ++ G +P+ + FK L+ + P PE Sbjct: 463164 EMQGVYISSRPVKISHATNNFKSQGALGTLAGTGLSQNLFNNNALPSIPTNIIPQTIPED 462985 Query: 96 ----------------YSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGY 139 +++VG+L+P+ D+ +L EFF G + + + Sbjct: 462984 LMPTTIITTDPLEQENTTVYVGNLSPNTDEKILREFFQGY------GPITSVKIPTNSNC 462823 Query: 140 GFVKFTDELEQKRALTECQGAVGLGSKPVRLS 171 GF+ FT +RA+ E G G++ VR+S Sbjct: 462822 GFINFTRTEHAERAIIEMNGIEIQGNR-VRVS 462730 >gb|GG738891.1| Naegleria gruberi genomic scaffold NAEGRscaffold_47, whole genome shotgun sequence Length = 339217 Score = 56.2 bits (134), Expect = 3e-08 Identities = 48/188 (25%), Positives = 87/188 (46%), Gaps = 8/188 (4%) Frame = -2 Query: 2 AASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKC 61 +ASL++GDL + E + F +G ++S++I R+ +T GY +V F + AE+ Sbjct: 283668 SASLYVGDLHEEISEALLFDLFHEVG-PIISIRICRDAMTRKSLGYAYVNFQNPQDAERA 283492 Query: 62 LHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFF----- 116 L +N + G ++ ++ S ++F+ +L +D+ LY+ F Sbjct: 283491 LDTLNYASVKGIP----IRIMWSQRDPSIRKSGIGNIFIKNLDKSIDNKALYDTFSAFGN 283324 Query: 117 ---VKVYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVA 173 KV + + G D S GYGFV F + ++A+ + G + L K V + Sbjct: 283323 ILSCKVCQATKKGDNGGDNV-ESAGYGFVHFETQEAAEKAIAKINGML-LNGKQVFVGPF 283150 Query: 174 IPKASRVK 181 + K R+K Sbjct: 283149 VKKTERLK 283126 Score = 56.2 bits (134), Expect = 3e-08 Identities = 53/193 (27%), Positives = 91/193 (47%), Gaps = 12/193 (6%) Frame = -2 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63 ++++ +L+ +DE +S F+ GE + + ++R G + F+ FAD A+A + Sbjct: 283098 NIYVKNLDASVDEKELSEVFSKFGE-IQNAVVMRGE-NGASKEFGFINFADHASALIAID 282925 Query: 64 KINGKPLPGAT---------PAKRFKLNYATYGKQPDNSPEY---SLFVGDLTPDVDDGM 111 ++N K +R KL + + +Y +L+V +L VDD Sbjct: 282924 EMNEKDFKNKKLFVGRAQKKNERRAKLKEYFQKLKQEKVNKYKGLNLYVKNLDDSVDDER 282745 Query: 112 LYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLS 171 L + F K + KV+ D S+G+GFV F +ALTE G + +GSKP+ ++ Sbjct: 282744 LRQEFSK-FGDITSAKVMSDNKQ-SRGFGFVCFKTPEAANKALTEMSGHM-IGSKPLYVN 282574 Query: 172 VAIPKASRVKPVE 184 A PK R +E Sbjct: 282573 FAQPKELRRSQLE 282535 Score = 47.0 bits (110), Expect = 2e-05 Identities = 42/161 (26%), Positives = 70/161 (43%), Gaps = 8/161 (4%) Frame = -2 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGI-------PAGYCFVEFADLA 56 ++++ +L+ +D + F+ G ++S K+ + G AGY FV F Sbjct: 283398 NIFIKNLDKSIDNKALYDTFSAFGN-ILSCKVCQATKKGDNGGDNVESAGYGFVHFETQE 283222 Query: 57 TAEKCLHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEY-SLFVGDLTPDVDDGMLYEF 115 AEK + KING L G + K N + +++V +L VD+ L E Sbjct: 283221 AAEKAIAKINGMLLNGKQVFVGPFVKKTERLKILSNEDSFTNIYVKNLDASVDEKELSEV 283042 Query: 116 FVKVYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTE 156 F K + + V+ + G SK +GF+ F D A+ E Sbjct: 283041 FSK-FGEIQNAVVMRGENGASKEFGFINFADHASALIAIDE 282922 >gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_3, whole genome shotgun sequence Length = 941567 Score = 54.7 bits (130), Expect = 1e-07 Identities = 53/182 (29%), Positives = 82/182 (45%), Gaps = 5/182 (2%) Frame = +3 Query: 3 ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62 A++++G+L+ +DE + F MG V+ KI R+R+T +GY FVEF A + Sbjct: 787725 ATIFVGNLDQQVDEELLWELFVQMGP-VVDAKIPRDRITNTHSGYAFVEFKHEHDANYAI 787901 Query: 63 HKINGKPLPGATPAKRFKLNYATYGKQPDN-SPEYSLFVGDLTP--DVDDGMLYEFFVKV 119 +N L G + KLN K N +L+VG+L P D+G+L E F + Sbjct: 787902 QVMNQIKLFG----RPMKLNRYDQDKSAKNLDVGANLWVGNLDPVGVSDEGILRELFGQF 788069 Query: 120 YPSCRGGKVVL--DQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKA 177 + + +T SKG+ FV + D E A + KP+ + A Sbjct: 788070 GVMIQNTPRIQRDPETMESKGFAFVSY-DNFESADAAKMHLNGQYISGKPIIVEYAFKPN 788246 Query: 178 SR 179 SR Sbjct: 788247 SR 788252 Score = 35.0 bits (79), Expect = 0.079 Identities = 20/75 (26%), Positives = 38/75 (50%), Gaps = 3/75 (4%) Frame = +3 Query: 1 MAASLWMGDLEP--YMDENFISRAFATMGETVMSV-KIIRNRLTGIPAGYCFVEFADLAT 57 + A+LW+G+L+P DE + F G + + +I R+ T G+ FV + + + Sbjct: 787986 VGANLWVGNLDPVGVSDEGILRELFGQFGVMIQNTPRIQRDPETMESKGFAFVSYDNFES 788165 Query: 58 AEKCLHKINGKPLPG 72 A+ +NG+ + G Sbjct: 788166 ADAAKMHLNGQYISG 788210 >gb|GG738960.1| Naegleria gruberi genomic scaffold NAEGRscaffold_116, whole genome shotgun sequence Length = 18323 Score = 45.4 bits (106), Expect = 6e-05 Identities = 31/88 (35%), Positives = 47/88 (53%), Gaps = 1/88 (1%) Frame = -2 Query: 95 EYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDELEQKRA 153 +Y ++ +L +V D ML FF + YPS V+ D+ T SKG+GF+ F D + A Sbjct: 12394 DYRIYANNLGNEVTDQMLTTFF-QSYPSFSKACVIRDKRTNRSKGFGFISFLDSNDYIHA 12218 Query: 154 LTECQGAVGLGSKPVRLSVAIPKASRVK 181 L G LG++P++L + K VK Sbjct: 12217 LENLNGKY-LGNRPLQLKPSKWKDRNVK 12137 >gb|GG738879.1| Naegleria gruberi genomic scaffold NAEGRscaffold_35, whole genome shotgun sequence Length = 384281 Score = 40.8 bits (94), Expect = 0.001 Identities = 27/76 (35%), Positives = 43/76 (56%), Gaps = 1/76 (1%) Frame = -3 Query: 97 SLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALT 155 +LF+ L DVDD L E F K + KV+ D +T +SKGYGFVK+ + A++ Sbjct: 302046 NLFIFHLPGDVDDSKLMELFSK-FGEIESVKVIRDPKTNLSKGYGFVKYCNIDSAMEAVS 301870 Query: 156 ECQGAVGLGSKPVRLS 171 + + +G K +++S Sbjct: 301869 K-MNSYKIGKKHLKVS 301825 >gb|GG738928.1| Naegleria gruberi genomic scaffold NAEGRscaffold_84, whole genome shotgun sequence Length = 160583 Score = 38.1 bits (87), Expect = 0.009 Identities = 23/66 (34%), Positives = 35/66 (53%) Frame = -1 Query: 2 AASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKC 61 ++++++ +L Y E I F GE V V + NR T P G+CFVEF + AE+C Sbjct: 132593 SSTIYVENLSFYTKEEQIYELFEKCGE-VKRVVMGLNRNTLTPCGFCFVEFYHRSDAEEC 132417 Query: 62 LHKING 67 + G Sbjct: 132416 VKYCRG 132399 >gb|GG738866.1| Naegleria gruberi genomic scaffold NAEGRscaffold_22, whole genome shotgun sequence Length = 469335 Score = 35.8 bits (81), Expect = 0.047 Identities = 20/57 (35%), Positives = 31/57 (54%) Frame = -3 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEK 60 +LW+G + + ++ + R F GE V+ VK+ G P GYCF+ F + AEK Sbjct: 392692 TLWLGGIPFHYNDMDLRRVFERFGE-VVDVKV--GYSGGQPLGYCFITFHERDAAEK 392531 >gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_16, whole genome shotgun sequence Length = 536960 Score = 35.0 bits (79), Expect = 0.079 Identities = 19/63 (30%), Positives = 30/63 (47%) Frame = +2 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 L++ L P E + F G ++ +I++NR TG Y F+EF D + E+ K Sbjct: 430682 LFVCRLNPITSEKSLRTIFLQYGN-IVKCEIVKNRKTGKSKTYAFIEFEDAESCERAFLK 430858 Query: 65 ING 67 G Sbjct: 430859 SEG 430867 >gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_1, whole genome shotgun sequence Length = 1555553 Score = 32.7 bits (73), Expect = 0.39 Identities = 16/70 (22%), Positives = 35/70 (50%) Frame = -1 Query: 3 ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62 A +++G L+ + E + F+ GE ++ + ++R G+CF+ + D+ + + Sbjct: 1298255 AYIYIGGLDFRLTEGDLLSVFSQYGE-IVDIDLVREEQELTSKGFCFIAYEDVRSTILAI 1298079 Query: 63 HKINGKPLPG 72 +NG L G Sbjct: 1298078 DNLNGIELGG 1298049 Score = 28.5 bits (62), Expect = 7.4 Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 1/57 (1%) Frame = +3 Query: 15 DENFISRAFATMGETV-MSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGKPL 70 D+ + R + GE + K + L GY F EFAD TA+K +NG P+ Sbjct: 337167 DDRSLKRMLSEFGEVSRVESKYEKRGLKKFRIGY-FAEFADKNTAKKVAITLNGSPV 337334 >gb|GG738851.1| Naegleria gruberi genomic scaffold NAEGRscaffold_7, whole genome shotgun sequence Length = 693645 Score = 32.3 bits (72), Expect = 0.52 Identities = 27/95 (28%), Positives = 49/95 (51%) Frame = +3 Query: 76 AKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGV 135 AK+ K +Y D Y++ + +L+ + D + E K Y S + D+ G Sbjct: 286644 AKKQKYDY-------DARAAYTVMLRNLSYNTSDDSIKEKLSK-YGSIVRVNIPTDERGR 286799 Query: 136 SKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRL 170 S+GYGFV+F DE+E + + + + A+ + + V+L Sbjct: 286800 SRGYGFVEF-DEVEAAQKVVDLK-AMEMDGREVQL 286898 >gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_14, whole genome shotgun sequence Length = 782252 Score = 32.0 bits (71), Expect = 0.67 Identities = 22/70 (31%), Positives = 33/70 (47%) Frame = +3 Query: 91 DNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQ 150 DN LF+ +L + L E F P R ++V++ G SKG+GF +F + E Sbjct: 145659 DNQQSTVLFIKNLNFKTTEDSLRELFKSYNP--RSVRIVVEN-GKSKGFGFAEFNNVKEA 145829 Query: 151 KRALTECQGA 160 +A E A Sbjct: 145830 VKAHEELHNA 145859 >gb|GG738877.1| Naegleria gruberi genomic scaffold NAEGRscaffold_33, whole genome shotgun sequence Length = 394700 Score = 31.2 bits (69), Expect = 1.1 Identities = 23/72 (31%), Positives = 35/72 (48%), Gaps = 6/72 (8%) Frame = -2 Query: 90 PDNSPEY---SLFVGDLTPDVDDGMLYEFFVKV---YPSCRGGKVVLDQTGVSKGYGFVK 143 PDN P + +FVG L + L +FF + Y ++ G S+G+GFV Sbjct: 56692 PDN-PSFINRRIFVGGLKETTSEQELIDFFSRSPNNYGPISHCVIMKKWDGRSRGFGFVT 56516 Query: 144 FTDELEQKRALT 155 F+DE K+ L+ Sbjct: 56515 FSDESIAKKVLS 56480 >gb|GG738889.1| Naegleria gruberi genomic scaffold NAEGRscaffold_45, whole genome shotgun sequence Length = 348446 Score = 29.6 bits (65), Expect = 3.3 Identities = 17/45 (37%), Positives = 26/45 (57%) Frame = -1 Query: 16 ENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEK 60 E I++ F+ G+ V SVKI+ +R I G+CFV A + A + Sbjct: 170042 EEDITKEFSLFGK-VESVKIVMDRKKLINRGFCFVTMASVEDATR 169911 >gb|GG738856.1| Naegleria gruberi genomic scaffold NAEGRscaffold_12, whole genome shotgun sequence Length = 602266 Score = 28.1 bits (61), Expect = 9.7 Identities = 18/54 (33%), Positives = 23/54 (42%) Frame = +1 Query: 32 SVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGKPLPGATPAKRFKLNYAT 85 S+ IRN I G+ F+ F + L K N KP+ KLN AT Sbjct: 28174 SMTCIRNSYQNIVIGFVFITFKQTCLLQSFLKK*NNKPI---------KLNLAT 28308 Database: N.gruberi/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 40,964,085 Number of sequences in database: 784 Lambda K H 0.317 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,405,998 Number of Sequences: 784 Number of extensions: 102364 Number of successful extensions: 379 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 32 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 306 Number of HSP's gapped (non-prelim): 121 length of query: 287 length of database: 13,654,695 effective HSP length: 101 effective length of query: 186 effective length of database: 13,575,511 effective search space: 2525045046 effective search space used: 2525045046 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 61 (28.1 bits)