TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74761781|sp|Q9NX07.1|TSAP1_HUMAN RecName: Full=tRNA selenocysteine 1-associated protein 1; AltName: Full=SECp43; AltName: Full=tRNA selenocysteine-associated protein 1 (287 letters) Database: H.arabidopsidis/genome.fa 5422 sequences; 67,459,135 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ABWE01000032.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 89 6e-18 gb|ABWE01000851.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 61 2e-09 gb|ABWE01001524.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 58 1e-08 gb|ABWE01000399.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 53 4e-07 gb|ABWE01000835.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 52 1e-06 gb|ABWE01000538.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 40 0.003 gb|ABWE01001149.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 39 0.011 gb|ABWE01000875.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 38 0.019 gb|ABWE01000774.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 34 0.22 gb|ABWE01003239.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 34 0.28 gb|ABWE01002583.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 32 0.82 gb|ABWE01000006.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 32 1.4 gb|ABWE01000458.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 31 2.4 gb|ABWE01000801.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 29 6.9 >gb|ABWE01000032.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont6.18, whole genome shotgun sequence Length = 116051 Score = 89.4 bits (220), Expect = 6e-18 Identities = 59/183 (32%), Positives = 91/183 (49%), Gaps = 38/183 (20%) Frame = +3 Query: 33 VKIIRNRLTGIPAGYCFVEFADLATAEKCLHKINGKPLPG-------------------- 72 +K+IR+++TG PAGY F+EF A++ L NG+ + Sbjct: 79764 IKLIRDKVTGYPAGYGFLEFPTQRGAQQVLEMFNGQLVRKNFCFFINQKSRYNFLFF*LI 79943 Query: 73 -----------------ATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEF 115 RF++N+ G++ + S ++S+FVGDL PDV D +L Sbjct: 79944 FVPILIILIFFGLMWCIENTVHRFRMNWGAGGRRIETSDDHSIFVGDLAPDVGDEVLLST 80123 Query: 116 FVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAI 174 F + S RG KVV+D T +SKG+GFV+F + E +AL G V S+P+R+SVA Sbjct: 80124 FNARFTSVRGAKVVMDPVTRMSKGFGFVRFGSKEEADQALQTMNG-VFCSSRPMRVSVAT 80300 Query: 175 PKA 177 ++ Sbjct: 80301 ERS 80309 Score = 31.6 bits (70), Expect = 1.4 Identities = 12/24 (50%), Positives = 18/24 (75%) Frame = +1 Query: 4 SLWMGDLEPYMDENFISRAFATMG 27 +LWMGD++ + DE FI+ FA+ G Sbjct: 79513 TLWMGDIQMHWDEAFIASLFASAG 79584 Score = 29.6 bits (65), Expect = 5.3 Identities = 18/64 (28%), Positives = 34/64 (53%) Frame = +3 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLH 63 ++++G L+P E + F +GE ++SVK+ R G FV++ +AE + Sbjct: 80367 TVFVGGLDPSATEEELRARFGALGE-IVSVKVPPGR------GCGFVQYTTKESAEMAIS 80525 Query: 64 KING 67 ++NG Sbjct: 80526 QMNG 80537 >gb|ABWE01000851.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont0.56, whole genome shotgun sequence Length = 25776 Score = 61.2 bits (147), Expect = 2e-09 Identities = 44/165 (26%), Positives = 82/165 (49%), Gaps = 1/165 (0%) Frame = -3 Query: 3 ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62 ASL++GD+ P + E + F +G V S+++ R+ +T GY +V F ++A AE+ L Sbjct: 9052 ASLYVGDIHPDVTEALLFEIFNAVGP-VASIRVCRDAVTRRSLGYAYVNFHNVADAERAL 8876 Query: 63 HKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPS 122 +N + ++ ++ S ++FV +L +D+ LY+ F ++ + Sbjct: 8875 DTMNFTSIKSVP----CRIMWSQRDPSLRKSGVGNIFVKNLDLSIDNKALYDTF-SLFGN 8711 Query: 123 CRGGKVVLDQT-GVSKGYGFVKFTDELEQKRALTECQGAVGLGSK 166 KV +D T G SKGYG+V + A+ + G + G++ Sbjct: 8710 ILSCKVAIDHTTGNSKGYGYVHYETAEAATEAIAKINGMLIAGTE 8576 Score = 43.1 bits (100), Expect = 5e-04 Identities = 36/101 (35%), Positives = 52/101 (51%), Gaps = 1/101 (0%) Frame = -3 Query: 71 PGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVL 130 P ATPA + AT QP ++ SL+VGD+ PDV + +L+E F V P +V Sbjct: 9109 PAATPAT----SAATGAAQPFHTA--SLYVGDIHPDVTEALLFEIFNAVGP-VASIRVCR 8951 Query: 131 DQ-TGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRL 170 D T S GY +V F + + +RAL + + S P R+ Sbjct: 8950 DAVTRRSLGYAYVNFHNVADAERAL-DTMNFTSIKSVPCRI 8831 >gb|ABWE01001524.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont13.27, whole genome shotgun sequence Length = 12533 Score = 58.2 bits (139), Expect = 1e-08 Identities = 37/83 (44%), Positives = 51/83 (61%), Gaps = 1/83 (1%) Frame = +3 Query: 90 PDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDEL 148 PDN ++ LF GDL +V D +L F K Y S + +VV D+ T S+GYGFV FTD Sbjct: 8826 PDN--DFRLFCGDLGNEVSDELLAHSFAK-YASFQRARVVRDKLTHKSRGYGFVSFTDPF 8996 Query: 149 EQKRALTECQGAVGLGSKPVRLS 171 + +AL E G +G++PV+LS Sbjct: 8997 DCAKALREMNGKY-IGNRPVKLS 9062 >gb|ABWE01000399.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont217.2, whole genome shotgun sequence Length = 44728 Score = 53.1 bits (126), Expect = 4e-07 Identities = 50/178 (28%), Positives = 86/178 (48%), Gaps = 1/178 (0%) Frame = +3 Query: 3 ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62 A++++ +L+ + E + G +V +V + R+++TG Y FVEF AE + Sbjct: 39315 ATVYVANLDDRVSEELLWELMLQTG-SVCNVHMPRDKITGSHQNYGFVEFRTEECAEYAV 39491 Query: 63 HKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPS 122 +N L G K ++ A+ K+ + +LF+G+L +VD+ +LY+ F Sbjct: 39492 KVLNMIQLYG----KVIRVKKASNEKKVLDVGA-NLFLGNLDAEVDEKLLYDTFSAFGGI 39656 Query: 123 CRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR 179 KV+ D T +S+G+GFV F D E EC L ++ V + A K S+ Sbjct: 39657 IETPKVMRDPDTRMSRGFGFVSF-DSFEAADLAIECMHGQYLCNRQVVVQYAYKKDSQ 39827 >gb|ABWE01000835.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont130.5, whole genome shotgun sequence Length = 26476 Score = 52.0 bits (123), Expect = 1e-06 Identities = 44/180 (24%), Positives = 82/180 (45%), Gaps = 3/180 (1%) Frame = +3 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 L++G + M+E+ + G ++ + IIR++LTG G F + AEK + + Sbjct: 11130 LFVGQVPRNMEEDELRPVLEIFG-SLEDLVIIRDKLTGAHRGCAFASYFTQEAAEKAVQE 11306 Query: 65 INGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCR 124 ++ K + P L Q S E+ LF+G + D+G + F +++ + Sbjct: 11307 LHNKVV---LPQSLNPLQVRPAEGQAGASQEHKLFIGMIPKGADEGAIRHVF-ELFGAIE 11474 Query: 125 GGKVVL-DQTGVSKGYGFVKFTDELEQKRALTECQGAVGL--GSKPVRLSVAIPKASRVK 181 ++ TG SKG F+KF D A+ + G V + G+ P+ + A + R++ Sbjct: 11475 EVYILRHPATGQSKGCAFLKFNDRASALAAIEQVNGIVTMDRGTAPLVVKFADSRRQRLQ 11654 Score = 33.9 bits (76), Expect = 0.28 Identities = 23/89 (25%), Positives = 45/89 (50%) Frame = +3 Query: 3 ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62 A+L++ L + + ++ AFA G TV+S K+ +++TG G+ FV + A+ + Sbjct: 12093 ANLFIYHLPHDLTDADLATAFAPFG-TVISAKVYMDKITGESKGFGFVSYDSADAADAAI 12269 Query: 63 HKINGKPLPGATPAKRFKLNYATYGKQPD 91 +NG + KR K+ + ++ D Sbjct: 12270 ASMNGFQI----GTKRLKVQHKRIHQRSD 12344 Score = 29.6 bits (65), Expect = 5.3 Identities = 22/75 (29%), Positives = 37/75 (49%), Gaps = 1/75 (1%) Frame = +3 Query: 97 SLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDELEQKRALT 155 +LF+ L D+ D L F + + KV +D+ TG SKG+GFV + D + A Sbjct: 12096 NLFIYHLPHDLTDADLATAFAP-FGTVISAKVYMDKITGESKGFGFVSY-DSADAADAAI 12269 Query: 156 ECQGAVGLGSKPVRL 170 +G+K +++ Sbjct: 12270 ASMNGFQIGTKRLKV 12314 >gb|ABWE01000538.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont56.14, whole genome shotgun sequence Length = 37306 Score = 40.4 bits (93), Expect = 0.003 Identities = 24/50 (48%), Positives = 29/50 (58%), Gaps = 1/50 (2%) Frame = -1 Query: 98 LFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTD 146 +FV L P VDD LYE F + KVV D +TG SKG+GFV F + Sbjct: 18883 VFVAGLPPHVDDNALYEKF-HAFGEMHQSKVVYDPKTGRSKGFGFVTFCE 18737 >gb|ABWE01001149.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont132.9, whole genome shotgun sequence Length = 18580 Score = 38.5 bits (88), Expect = 0.011 Identities = 32/105 (30%), Positives = 44/105 (41%), Gaps = 9/105 (8%) Frame = -2 Query: 87 GKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVKFTD 146 G + DN P LFV +L + L E Y + LD+T KGYGFV+F Sbjct: 15696 GTEDDNKPSARLFVRNLPFTAVEEDL-EALCAAYGPVEEVHMPLDETRRRKGYGFVRFRT 15520 Query: 147 ELEQKRALTECQG---------AVGLGSKPVRLSVAIPKASRVKP 182 ++ + A T G + SKPV+L P+ S P Sbjct: 15519 TVDAQTACTAMNGLAFQGRRLHVISARSKPVKLD---PEVSLADP 15394 >gb|ABWE01000875.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont28.10, whole genome shotgun sequence Length = 25126 Score = 37.7 bits (86), Expect = 0.019 Identities = 45/148 (30%), Positives = 69/148 (46%), Gaps = 5/148 (3%) Frame = -3 Query: 2 AASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKC 61 A +++ L E ++ FA GE V S +I + G G FV FA AE Sbjct: 19226 ALEIFIAGLPWAATEEEVTEYFAGCGE-VTSARIPQQN--GRSTGTAFVTFATPEAAEAG 19056 Query: 62 LHKINGKPLPGATPAKRFKLNYATYGKQPDNSPE--YSLFVGDLTPDVDDGMLYEFFVKV 119 L ++G+ G + K+ A D PE S+F+G+L+ DVD+ ++ E F + Sbjct: 19055 L-ALDGQDFGG----RWLKIRTAEKKNMFDEKPEGCTSVFIGNLSWDVDEDIIRETFGEC 18891 Query: 120 --YPSCRGGKVVLD-QTGVSKGYGFVKF 144 SCR + D +TG +G+G V F Sbjct: 18890 GEILSCR---LATDRETGEFRGFGHVDF 18816 >gb|ABWE01000774.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont31.12, whole genome shotgun sequence Length = 28357 Score = 34.3 bits (77), Expect = 0.22 Identities = 21/63 (33%), Positives = 33/63 (52%) Frame = +3 Query: 97 SLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGYGFVKFTDELEQKRALTE 156 +LFVG ++ + + L F + P R ++V D G S+GYGFV++ E + K A Sbjct: 3087 TLFVGRISYETTEQQLRHEFEQYGPVKRV-RLVEDPDGKSRGYGFVEYEKEADMKAAYKY 3263 Query: 157 CQG 159 G Sbjct: 3264 ADG 3272 >gb|ABWE01003239.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont1489.2, whole genome shotgun sequence Length = 1991 Score = 33.9 bits (76), Expect = 0.28 Identities = 18/60 (30%), Positives = 34/60 (56%) Frame = -2 Query: 2 AASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKC 61 +++L++G+L + E + F+ +G V V + +R P G+CFVE++ + AE C Sbjct: 688 SSTLYVGNLSFFTSEAQLYELFSRVGY-VKRVIVGLDRFKRTPCGFCFVEYSTHSEAEAC 512 >gb|ABWE01002583.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont85.16, whole genome shotgun sequence Length = 2904 Score = 32.3 bits (72), Expect = 0.82 Identities = 17/59 (28%), Positives = 30/59 (50%) Frame = -2 Query: 4 SLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62 S+++G ++ + F + G T+ V I+ ++ TG P GY ++EFA E L Sbjct: 377 SIYIGQVDYGSTPEELQALFQSCG-TINRVTILCDKFTGQPKGYAYIEFASRDAVESAL 204 >gb|ABWE01000006.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont26.1, whole genome shotgun sequence Length = 176035 Score = 31.6 bits (70), Expect = 1.4 Identities = 11/25 (44%), Positives = 17/25 (68%) Frame = +3 Query: 46 GYCFVEFADLATAEKCLHKINGKPL 70 G+CF+E+ D+ AE + +NG PL Sbjct: 15018 GFCFLEYEDVLAAESAVQVLNGTPL 15092 >gb|ABWE01000458.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont66.5, whole genome shotgun sequence Length = 41258 Score = 30.8 bits (68), Expect = 2.4 Identities = 11/28 (39%), Positives = 19/28 (67%) Frame = +2 Query: 136 SKGYGFVKFTDELEQKRALTECQGAVGL 163 SKG+GF++F DE++ + A+ G + L Sbjct: 37760 SKGFGFIEFVDEMDARAAIDNMDGRIVL 37843 >gb|ABWE01000801.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont25.18, whole genome shotgun sequence Length = 27371 Score = 29.3 bits (64), Expect = 6.9 Identities = 25/74 (33%), Positives = 33/74 (44%), Gaps = 3/74 (4%) Frame = +2 Query: 8 GDLEPYM-DENFISRAFATMGETVMSVKI-IRNRLTGIPAGYCFVEFADLATAEK-CLHK 64 G+ EPY D + S T E + V + IR+R FV A+ A CLHK Sbjct: 6572 GNTEPYQCDVIYNSSVTCTKQEWYVRVNVRIRHRQ--------FVSKAEPQVANSTCLHK 6727 Query: 65 INGKPLPGATPAKR 78 +N +P TP R Sbjct: 6728 LNASTIPTQTPTPR 6769 Database: H.arabidopsidis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 67,459,135 Number of sequences in database: 5422 Lambda K H 0.317 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,274,723 Number of Sequences: 5422 Number of extensions: 199802 Number of successful extensions: 866 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 280 Number of HSP's successfully gapped in prelim test: 30 Number of HSP's that attempted gapping in prelim test: 550 Number of HSP's gapped (non-prelim): 385 length of query: 287 length of database: 22,486,378 effective HSP length: 104 effective length of query: 183 effective length of database: 21,922,490 effective search space: 4011815670 effective search space used: 4011815670 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 63 (28.9 bits)