TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|74761781|sp|Q9NX07.1|TSAP1_HUMAN RecName: Full=tRNA selenocysteine 1-associated protein 1; AltName: Full=SECp43; AltName: Full=tRNA selenocysteine-associated protein 1 (287 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668641.1| Blastocystis hominis, Singapore isolate B (sub-t... 49 1e-09 emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-t... 49 2e-06 emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-t... 38 0.006 emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-t... 35 0.038 emb|FN668672.1| Blastocystis hominis, Singapore isolate B (sub-t... 35 0.049 emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-t... 32 0.24 emb|FN668688.1| Blastocystis hominis, Singapore isolate B (sub-t... 32 0.32 emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 1.2 emb|FN668642.1| Blastocystis hominis, Singapore isolate B (sub-t... 30 1.6 >emb|FN668641.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_11 Length = 591523 Score = 49.3 bits (116), Expect(2) = 1e-09 Identities = 37/127 (29%), Positives = 55/127 (43%), Gaps = 18/127 (14%) Frame = -1 Query: 23 FATMGETVMSVKIIRNRLTGIPAGYCFVE-------FADLATAEKCLHKING---KPLPG 72 +A E + S+K++R+R T G F F D AT E +H +NG +P+ Sbjct: 355501 YAKESEGLSSIKLMRDRTTNESLGLHFSTCLRLGFGFIDFATEEDAIHALNGYNGRPIRF 355322 Query: 73 --------ATPAKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCR 124 A F+LN+ + + Y L++GDL V D LY F Y S Sbjct: 355321 LVWFL*LLAGTGYTFRLNFGGNSRNLNLGDNYCLYIGDLESSVTDTQLYTIFRDKYLSFC 355142 Query: 125 GGKVVLD 131 G KV+ + Sbjct: 355141 GAKVLFN 355121 Score = 30.4 bits (67), Expect(2) = 1e-09 Identities = 14/27 (51%), Positives = 17/27 (62%) Frame = -3 Query: 133 TGVSKGYGFVKFTDELEQKRALTECQG 159 T VSKGYGF++F E + AL E G Sbjct: 355082 TSVSKGYGFIQFRARDEAETALKEMNG 355002 >emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_0 Length = 1966360 Score = 49.3 bits (116), Expect = 2e-06 Identities = 30/80 (37%), Positives = 46/80 (57%), Gaps = 1/80 (1%) Frame = -3 Query: 92 NSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGV-SKGYGFVKFTDELEQ 150 N ++ LFVG+L +V +L F YPS G+VV + S GYGFV F++ LE Sbjct: 688652 NEKDFRLFVGNLGREVTTEILSNLFRMKYPSFTMGRVVESKVHKGSAGYGFVAFSNALEG 688473 Query: 151 KRALTECQGAVGLGSKPVRL 170 +A+ E G + GS+P+++ Sbjct: 688472 VKAMKEMNGKL-CGSRPMKI 688416 Score = 31.2 bits (69), Expect = 0.54 Identities = 19/73 (26%), Positives = 37/73 (50%) Frame = -3 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 L++G+L + +S F + +++ +++ AGY FV F++ K + + Sbjct: 688634 LFVGNLGREVTTEILSNLFRMKYPSFTMGRVVESKVHKGSAGYGFVAFSNALEGVKAMKE 688455 Query: 65 INGKPLPGATPAK 77 +NGK L G+ P K Sbjct: 688454 MNGK-LCGSRPMK 688419 Score = 27.7 bits (60), Expect = 6.0 Identities = 32/101 (31%), Positives = 47/101 (46%), Gaps = 2/101 (1%) Frame = +1 Query: 58 AEKCLHKINGKPLPGATP-AKRFKLNYATYGKQPDNSPEYSLFVGDLTPDVDDGMLYEFF 116 AEK L N L G R +L + KQ N P+ +LFV +L + + L + F Sbjct: 1484599 AEKALEGANFNILKGKEMRVMRQQLRF----KQTCN-PKGNLFVKNLPDLMTNKELLDKF 1484763 Query: 117 VKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALTE 156 Y + KV D +TG S YG+V F+D ++ L + Sbjct: 1484764 -SAYGNILSCKVAFDKETGKSLHYGYVHFSDPNVTQKVLAD 1484883 >emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_1 Length = 1866585 Score = 37.7 bits (86), Expect = 0.006 Identities = 43/149 (28%), Positives = 61/149 (40%), Gaps = 27/149 (18%) Frame = -2 Query: 55 LATAEKCLHKINGKPLPGATPAKRFKLNYATYGKQPDNSP-----------------EYS 97 + T+E L G+P+ GA +AT G + P + + Sbjct: 1614800 MMTSEGVLVPATGQPIEGAAA-------FATAGFGREEGPPRRESEGGGRA*RVGPADAN 1614642 Query: 98 LFVGDLTPDVDDGMLYEFF--------VKVYPSCRGGK-VVLD-QTGVSKGYGFVKFTDE 147 LFV ++ + D L + F KV G V+LD QT VSKGYGFV F + Sbjct: 1614641 LFVFNIPSEFKDADLEDLFREFGDLINAKVGERKEGEA*VILDRQTSVSKGYGFVNFATK 1614462 Query: 148 LEQKRALTECQGAVGLGSKPVRLSVAIPK 176 RA++ G V G K +SV + K Sbjct: 1614461 EAADRAISAMNGKVIRGKK---ISVQVKK 1614384 >emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_3 Length = 1354787 Score = 35.0 bits (79), Expect = 0.038 Identities = 29/87 (33%), Positives = 42/87 (48%) Frame = +2 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 L++G + DE+ + F G V SV+IIRNR TGI G+ FV F A + H Sbjct: 591170 LFVGGIPNEADESVLRAYFQYFGP-VRSVEIIRNRETGISRGFGFVVFYSRIVAVR--HA 591340 Query: 65 INGKPLPGATPAKRFKLNYATYGKQPD 91 +G L A R + +G++ D Sbjct: 591341 DDG*SLEDALQQTRIEF----FGRKVD 591409 Score = 33.1 bits (74), Expect = 0.14 Identities = 17/55 (30%), Positives = 33/55 (60%), Gaps = 1/55 (1%) Frame = +2 Query: 91 DNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKF 144 D + LFVG + + D+ +L +F + + R +++ + +TG+S+G+GFV F Sbjct: 591149 DPIDDCKLFVGGIPNEADESVLRAYF-QYFGPVRSVEIIRNRETGISRGFGFVVF 591310 Score = 31.2 bits (69), Expect = 0.54 Identities = 21/70 (30%), Positives = 30/70 (42%), Gaps = 8/70 (11%) Frame = -3 Query: 5 LWMGDLEPYMDENFISRAFATMGE--------TVMSVKIIRNRLTGIPAGYCFVEFADLA 56 L++ L P ++ + F G T +KIIR+ TG GY F+EF Sbjct: 1171527 LFVCQLNPITEDEDLKTIFYRFGVKSCEVVE*TGRELKIIRDHETGNSLGYAFIEFESNE 1171348 Query: 57 TAEKCLHKIN 66 AE K+N Sbjct: 1171347 AAEDAYVKMN 1171318 Score = 28.9 bits (63), Expect = 2.7 Identities = 28/73 (38%), Positives = 34/73 (46%), Gaps = 15/73 (20%) Frame = -3 Query: 87 GKQPD---NSPEYSLFVGDLTPDVDDGML----YEFFVKVYPSC-------RGGKVVLD- 131 G PD PE LFV L P +D L Y F VK SC R K++ D Sbjct: 1171569 GDLPDADVKPPENVLFVCQLNPITEDEDLKTIFYRFGVK---SCEVVE*TGRELKIIRDH 1171399 Query: 132 QTGVSKGYGFVKF 144 +TG S GY F++F Sbjct: 1171398 ETGNSLGYAFIEF 1171360 >emb|FN668672.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_4 Length = 1031885 Score = 34.7 bits (78), Expect = 0.049 Identities = 27/77 (35%), Positives = 39/77 (50%), Gaps = 1/77 (1%) Frame = +1 Query: 97 SLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALT 155 +LFV L ++ D L FV Y + KV +D QTG SKG+GFV F + A+ Sbjct: 886006 NLFVYHLPHEMADSDLTTLFVP-YGTILSAKVYVDKQTGESKGFGFVSFNSFEAAQEAIR 886182 Query: 156 ECQGAVGLGSKPVRLSV 172 G + SK +++ V Sbjct: 886183 HMNG-FQIDSKRLKVQV 886230 >emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_2 Length = 1458350 Score = 32.3 bits (72), Expect = 0.24 Identities = 20/60 (33%), Positives = 29/60 (48%) Frame = -3 Query: 7 MGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHKIN 66 +G L+ +DE + F GE V V I + TG+ G+ FVEF D A + +N Sbjct: 965703 IGGLDDQVDEQVLLGVFVPFGE-VKEVTIPLDAGTGLKRGFGFVEFEDAEDAAEARDNMN 965527 >emb|FN668688.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_6 Length = 900598 Score = 32.0 bits (71), Expect = 0.32 Identities = 19/68 (27%), Positives = 34/68 (50%) Frame = +3 Query: 5 LWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCLHK 64 +++G + +E + + F GE V +++ RN+ TG Y F+EF AE ++ Sbjct: 584670 IYLGHIPTEFEEPQMRKFFEQFGE-VKRLRLSRNKKTGASKHYAFIEFETPQIAEIVMNT 584846 Query: 65 INGKPLPG 72 +N L G Sbjct: 584847 MNNYILFG 584870 >emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_5 Length = 953317 Score = 30.0 bits (66), Expect = 1.2 Identities = 30/134 (22%), Positives = 57/134 (42%), Gaps = 20/134 (14%) Frame = -2 Query: 3 ASLWMGDLEPYMDENFISRAFATMG---------ETVMSV-----KIIRNRLTGIPA--- 45 A+L++G L+ +DE + F +G V+SV K++ + G+ + Sbjct: 741870 ATLYVGGLDERVDEELLWELFLQVGIDWCCAF*FGPVVSVSMPKDKVLNKHMVGMGS*ND 741691 Query: 46 ---GYCFVEFADLATAEKCLHKINGKPLPGATPAKRFKLNYATYGKQPDNSPEYSLFVGD 102 + F+E+ AE H + L G ++ ++N + + P LF+ + Sbjct: 741690 SL*EFAFIEYQSEIDAEYASHVCDNITLYG----RKIRVNKSNKDR-PTLDVGADLFISN 741526 Query: 103 LTPDVDDGMLYEFF 116 L PDV + M+ F Sbjct: 741525 LAPDVTEDMIKTTF 741484 >emb|FN668642.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_12 Length = 491035 Score = 29.6 bits (65), Expect = 1.6 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = +1 Query: 132 QTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSK 166 QTG S+G G VKF+ E A++ G+V G + Sbjct: 158548 QTGRSRGMGIVKFSTREEMNNAISTMNGSVRNGEE 158652 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.317 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,653,512 Number of Sequences: 54 Number of extensions: 52867 Number of successful extensions: 232 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 192 Number of HSP's gapped (non-prelim): 121 length of query: 287 length of database: 6,272,415 effective HSP length: 96 effective length of query: 191 effective length of database: 6,267,231 effective search space: 1197041121 effective search space used: 1197041121 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.9 bits)