TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= TR1 #anophagefferens # scaffold_11:subseq(783554,7436).pep (488 letters) Database: P.ultimum/genome.fa 82 sequences; 42,801,618 total letters Searching.................................................................................done Score E Sequences producing significant alignments: (bits) Value gb|GL376634.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 441 e-124 gb|GL376632.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 208 9e-54 gb|GL376566.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 173 3e-43 gb|GL376629.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 148 1e-35 gb|GL376611.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 43 6e-04 gb|GL376573.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 35 0.22 gb|GL376626.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 30 5.5 >gb|GL376634.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582037, whole genome shotgun sequence Length = 1414051 Score = 441 bits (1134), Expect = e-124 Identities = 246/493 (49%), Positives = 305/493 (61%), Gaps = 6/493 (1%) Frame = -3 Query: 2 FDYDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLM 61 +DYD LACSKE AK G KV VLD+VKPS G++WGLGGTCVNVGCIPKKLM Sbjct: 1305437 YDYDLVVIGGGSGGLACSKEAAKYGKKVCVLDYVKPSLVGTSWGLGGTCVNVGCIPKKLM 1305258 Query: 62 HTASIIGETLXXXXXXXXXXXXXXXXHDWESMKSTVQDHIKSLNFKYRVALREAGITYLN 121 H +S+IGET+ DW M +V +IKS+NFKY+V LR+ + Y N Sbjct: 1305257 HQSSLIGETIHKDSKAFGWTVGEQTF-DWAQMLKSVNSYIKSINFKYKVELRDKKVKYEN 1305081 Query: 122 RLGEFEDKNTISLTD-KKGNKTSVTAARFIVAVGGRPNKLGCPGGEHAIDSDDLFSLPKA 180 LG F D +T+ L KKG KT VT ++AVGGRP L C GG+ AI SDD+F +A Sbjct: 1305080 LLGSFIDAHTLELWHPKKGTKT-VTTRDVVIAVGGRPKPLACEGGDLAISSDDIFWRKEA 1304904 Query: 181 PGRVLCVGGGYIALECGGFTAGMGYPTTCMVRSILLRGFDRECVGKI----EAHIKHHGV 236 PG+ L VG Y+ALEC GF GMGY MVRSILLRGFD++ KI E K + Sbjct: 1304903 PGKTLVVGASYVALECAGFMKGMGYDVKIMVRSILLRGFDQDMADKIGEFMEVESKIEFI 1304724 Query: 237 QLQVGVTPAKIEKDAATGVLTVTDSGGGVHEYDTVLVATGRYADTAALGIDNVPGASANV 296 + V + K+E T D +DTVL ATGR D A LG+D A + Sbjct: 1304723 RGCVPQSIMKLENGQLLVKWTTADGEACEEAFDTVLNATGRDPDVAKLGLDK---AGVKL 1304553 Query: 297 NAKTKKLECVDEQLPGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTEPMDYEC 356 N KT ++ +EQ P+VYAIGDV++ PELTPVAI+AG LARRL+ +T MDY+ Sbjct: 1304552 NPKTGRIWVENEQ-TSTPNVYAIGDVIDA-PELTPVAIQAGRLLARRLYNNETTLMDYDK 1304379 Query: 357 VATTIFTPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLV 416 V T +FTP+EYG GLSE+ + + GE N+E Y F PLE++LS C+ K++ Sbjct: 1304378 VCTAVFTPIEYGCCGLSEEASIEKFGEDNLEIYHQNFTPLEWSLSSATRPAASSCYVKVI 1304199 Query: 417 VDKSTGK-VVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEFTSMTVTK 475 DKS K VVGFHYLGPNAGE+TQ +AM+ G TY ++ VGIHPT AE FT++ VTK Sbjct: 1304198 CDKSRDKYVVGFHYLGPNAGEVTQAMGLAMKLGFTYDQMVNVVGIHPTTAEIFTTLEVTK 1304019 Query: 476 SSGESAAKGGCUG 488 SSG SAA GGC G Sbjct: 1304018 SSGASAAAGGC*G 1303980 >gb|GL376632.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582035, whole genome shotgun sequence Length = 696189 Score = 208 bits (530), Expect = 9e-54 Identities = 161/478 (33%), Positives = 225/478 (47%), Gaps = 10/478 (2%) Frame = +1 Query: 4 YDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLMHT 63 YD +A ++ A GAKV V++ + GLGGTCVN GC+PKK+M Sbjct: 427336 YDYIAIGGGSGGMASARRAATYGAKVLVVERGRKYDGA---GLGGTCVNYGCVPKKVMFN 427506 Query: 64 ASIIGETLXXXXXXXXXXXXXXXXH---DWESMKSTVQDHIKSLNFKYRVALREAGITYL 120 A+ E L DW++MK+ +I LN Y L A I + Sbjct: 427507 AASHVEHLHRNADYSITTTTKPFEFGDFDWKNMKTKRDAYIARLNGIYERNLGNAQIDLV 427686 Query: 121 NRLGEFEDKNTISLTDKKGNKTSVTAARFIVAVGGRPNKLGCPGGEHAIDSDDLFSLPKA 180 + + K+ + + + T +VAVGG P+ PG EH IDSDD F L + Sbjct: 427687 EGVAKLVAKDKVEV-----DGVVYTGKHILVAVGGVPSLPPIPGIEHVIDSDDFFKLEEQ 427851 Query: 181 PGRVLCVGGGYIALECGGFTAGMGYPTTCMVR-SILLRGFDRECVGKIEAHIKHHGVQLQ 239 P +V VG GYIA+E G + T+ R +LR FD I + G+ Sbjct: 427852 PKKVAVVGAGYIAIEIAGIFNALKSDTSLFCRFDHVLRTFDPIVRDLINEEGEKAGINFV 428031 Query: 240 VGVTPAKIEKDAATGVLTVTDSGGGVHE----YDTVLVATGRYADTAALGIDNVPGASAN 295 +I+K A G L +T G + ++ +L A GR T LG++ V Sbjct: 428032 RNSRIERIDKQ-ADGTLQITAKVNGEEQVIGGFNQILYAIGREPRTKDLGLEEV-----G 428193 Query: 296 VNAKTKKLECVD-EQLPGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTEP-MD 353 V K VD ++ +YAIGD ELTPVAI AG RL+ RLFGG+ + Sbjct: 428194 VKLNEKGFIVVDAQENTSVSGIYAIGDATTTGWELTPVAIAAGRRLSDRLFGGEPNACLH 428373 Query: 354 YECVATTIFTPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFA 413 Y + T +F+ GTIG +E +A + G NV++Y S+F L YA+++ +G Sbjct: 428374 YHQIPTVVFSHPPLGTIGYTEPEAIEKFGAENVKTYSSKFVNLLYAMADP-DHKGKTAM- 428547 Query: 414 KLVVDKSTGKVVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEFTSM 471 KLV VVG H G A E+ QGF +A++ AT ADF + V IHPT AEE +M Sbjct: 428548 KLVTVGEDEVVVGIHVAGEGADEMIQGFGVAVKMNATKADFDNIVAIHPTAAEELVTM 428721 >gb|GL376566.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581352, whole genome shotgun sequence Length = 165876 Score = 173 bits (439), Expect = 3e-43 Identities = 125/365 (34%), Positives = 179/365 (49%), Gaps = 37/365 (10%) Frame = -1 Query: 144 VTAARFIVAVGGRPNKLGCPGGEHAIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGM 203 V A ++AVGG+P PG EH IDSD F L P +V VG GYIA+E G G+ Sbjct: 133803 VKAKNILIAVGGKPLIPDIPGKEHCIDSDGFFELEVLPKKVAVVGAGYIAVELAGVLNGL 133624 Query: 204 GYPTTCMVRSI-LLRGFDRECVGKIEAHI-------------------------KHHGVQ 237 G TT R +LR FD ++ H+ G+ Sbjct: 133623 GSDTTIFCRKHGVLRTFDVSRSVLLDVHLTLMYPALNLSIFM*DSVRDMLHDAMAKDGIT 133444 Query: 238 LQVGVTPAKIEKDAATGVLTVTDSGGGVHE-YDTVLVATGRYADTAALGIDNVPGASANV 296 L+ A++E AA G T+ G H +DT++ A GR T++L + ++A + Sbjct: 133443 LRANSNVARVEL-AADGTKTLALQDGSEHTGFDTIIYAAGRVPLTSSLNL-----SAAGI 133282 Query: 297 NAKTKKLECVDE-QLPGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTE---PM 352 + VD Q +V+A+GDV G LTPVAI AG RL+ RLFGG+ + Sbjct: 133281 KTDSHGFIKVDAYQATSKSNVFAVGDVC-GTTALTPVAIAAGRRLSDRLFGGERHADAKI 133105 Query: 353 DYECVATTIFTPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGC- 411 Y+ + T +F+ GTIGL+E +A+ + G+ +++Y S F + Y L ++ G Sbjct: 133104 SYDNIPTVVFSHPPIGTIGLTEIEARKKYGDAAIKTYTSRFVNMYYGLVNEVADDGKAAK 132925 Query: 412 -----FAKLVVDKSTGKVVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAE 466 KLV KVVG H +G A E+ QGF +A++ GAT ADF + + IHPT AE Sbjct: 132924 PKPMTAMKLVCLGDDEKVVGLHIIGMAADEMLQGFGVAVKMGATKADFDNCIAIHPTGAE 132745 Query: 467 EFTSM 471 E +M Sbjct: 132744 ELVTM 132730 Score = 41.6 bits (96), Expect = 0.002 Identities = 24/70 (34%), Positives = 36/70 (51%), Gaps = 1/70 (1%) Frame = -2 Query: 3 DYDXXXXXXXXXXLACSKECAKL-GAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLM 61 +YD +A S+ A +VAV++ + LGGTCVNVGC+PKK+M Sbjct: 134291 EYDYLVIGAGSGGMASSRRAASYPNTRVAVVEKAR---------LGGTCVNVGCVPKKIM 134139 Query: 62 HTASIIGETL 71 + A+ + L Sbjct: 134138 YLAADMNHML 134109 >gb|GL376629.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582032, whole genome shotgun sequence Length = 863081 Score = 148 bits (374), Expect = 1e-35 Identities = 132/477 (27%), Positives = 209/477 (43%), Gaps = 11/477 (2%) Frame = +3 Query: 3 DYDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKK-LM 61 DYD + + A+LG K A ++ S LGGTC+NVGCIP K L+ Sbjct: 652239 DYDVVVIGGGPGGYVAAIKAAQLGLKTACIE--------SRGTLGGTCLNVGCIPSKALL 652394 Query: 62 HTASIIGETLXXXXXXXXXXXXXXXXHDWESMKSTVQDHIKSLNFKYRVALREAGITYLN 121 H+ + D+ + + +K+L ++ +TY+ Sbjct: 652395 HSTHLYHSAQHDFSKYGIEVSGVSM--DFGKLMKSKDKAVKTLTGGIEQLFKKNKVTYIK 652568 Query: 122 RLGEFEDKNTISLTDKKGNKTSVTAARFIVAVGGRPNKLGCPGGEHA----IDSDDLFSL 177 G+ KN I++T +V+A ++A G L ++A +DS L Sbjct: 652569 GHGKIVAKNEIAVTLNDSGNQTVSAKNILIATGSEVTPLPPVPVDNAAGKIVDSTGALEL 652748 Query: 178 PKAPGRVLCVGGGYIALECGGFTAGMGYPTTCM-VRSILLRGFDRECVGKIEAHIKHHGV 236 K P ++ +G G I LE G +G T + + G D E + +K G+ Sbjct: 652749 KKVPEHLVVIGAGVIGLELGSVYKRLGAKVTVVEYLDRVCPGLDLELGKEFTKLLKKQGL 652928 Query: 237 --QLQVGVTPAKIEKDAATGVLTVTDSGGGVH---EYDTVLVATGRYADTAALGIDNVPG 291 + VT +K++ D T LT + GG E D VLVATGR T LG++N+ G Sbjct: 652929 GFEFSTKVTASKVDGDKVT--LTTEPAAGGAASTIECDAVLVATGRRPFTGGLGLENM-G 653099 Query: 292 ASANVNAKTKKLECVDEQLPGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTEP 351 + + + E Q+P +++AIGDV+ G P L A E G+ + GK Sbjct: 653100 IQTDKFGRIQVDEHFRTQIP---NIFAIGDVIAG-PMLAHKAEEEGIACVETI-AGKHGH 653264 Query: 352 MDYECVATTIFTPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGC 411 ++Y + I+T E ++G +E+ QL E V + +F L + + E DG Sbjct: 653265 VNYNAIPGVIYTFPEVASVGKTEE----QLKEEGVAYNVGKFPMLANSRARAVGE-ADG- 653426 Query: 412 FAKLVVDKSTGKVVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEF 468 K++ DK+T K++G H + NAGE+ + M GA D T HPT++E F Sbjct: 653427 LVKVIADKATDKILGVHIIASNAGEMISEGVLGMEYGAASEDIARTCHAHPTLSEAF 653597 >gb|GL376611.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582014, whole genome shotgun sequence Length = 494327 Score = 43.1 bits (100), Expect = 6e-04 Identities = 40/156 (25%), Positives = 69/156 (44%), Gaps = 4/156 (2%) Frame = +2 Query: 317 YAIGDVVEGRPELTPVAIEAGLRLARRLFGGK----TEPMDYECVATTIFTPLEYGTIGL 372 YA+GD + G P L ++E G A ++ + T ++ I+T E +G Sbjct: 330518 YAVGDCI-GAPSLASTSMEQGRLAACHMWDQEEQLVTSQLENGNYPYGIYTIPEISMVGK 330694 Query: 373 SEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTGKVVGFHYLGP 432 +E + N E I++++ E A + G K++ D +T K+ G H +G Sbjct: 330695 TEQQLTKE--GVNFEIGIAKYS--ELAKGQMSGSTAGGML-KILFDPTTLKLFGVHAIGE 330859 Query: 433 NAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEF 468 A EI +A+ G + F V +PT+AE + Sbjct: 330860 GATEIIHIGQVAIAMGCSVTYFRDAVFNYPTLAEAY 330967 Score = 34.7 bits (78), Expect = 0.22 Identities = 20/62 (32%), Positives = 26/62 (41%) Frame = +3 Query: 3 DYDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLMH 62 DYD C+ E AK G +VA++D +GG CV+ G IP K Sbjct: 329151 DYDLVVIGSGPSATQCAIESAKQGKRVAIVD--------KKTSVGGVCVHTGTIPSKTFR 329306 Query: 63 TA 64 A Sbjct: 329307 EA 329312 >gb|GL376573.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581402, whole genome shotgun sequence Length = 979788 Score = 34.7 bits (78), Expect = 0.22 Identities = 48/170 (28%), Positives = 72/170 (42%), Gaps = 18/170 (10%) Frame = +3 Query: 317 YAIGDVVEGRPELTPVAIEAGLRLARRLFG--------GKTEPMDYECVATT-------- 360 YA GD + G P L ++E G RLA G +E D + + T Sbjct: 622509 YAAGDCI-GFPALASTSMEQG-RLASVHMRTEKPTNKTGDSESTDPQFMRTRMRSGDVFP 622682 Query: 361 --IFTPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVD 418 I+T E +G +E QL + NV Y A E L++ + G F K++ Sbjct: 622683 FGIYTVPEISMVGKNEQ----QLTKENVP-YEVGLARYE-ELAKGQMLGGVPGFLKIIFC 622844 Query: 419 KSTGKVVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEF 468 T +++G H +G A EI + M G T F + V +PT+AE + Sbjct: 622845 PKTLQLLGVHAIGEGATEIIHIGQVVMSTGGTIEYFRNAVFNYPTLAEAY 622994 Score = 29.6 bits (65), Expect = 7.2 Identities = 34/130 (26%), Positives = 48/130 (36%), Gaps = 20/130 (15%) Frame = +1 Query: 207 TTCMVRSILLRGFDRECVGKIEAHIKHHGVQLQVGVTPAKIEK------------DAATG 254 T C+ I E + I IKHH L V + I++ +A Sbjct: 764236 TACLWSFICDAAIHHERINPIPVRIKHHEYTLWVSASSISIKRHFRAPSM*VSHSGSAAD 764415 Query: 255 VLTVTDSGGGVHEYDTVLVATGRYADTAALGIDNVPGASANV------NAKTKKLECVDE 308 L+ D+ + +T LV R AL +VP N NA TK L+ + Sbjct: 764416 PLSTFDTYQEIINVNTGLVVASRQHFQQALA-SHVPALGRNARPRRLSNAGTKSLQLDPK 764592 Query: 309 QLP--GAPHV 316 LP APH+ Sbjct: 764593 TLPCSRAPHL 764622 >gb|GL376626.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582029, whole genome shotgun sequence Length = 1550222 Score = 30.0 bits (66), Expect = 5.5 Identities = 12/36 (33%), Positives = 22/36 (61%) Frame = +3 Query: 213 SILLRGFDRECVGKIEAHIKHHGVQLQVGVTPAKIE 248 ++LLR +D ECV E ++H+G + +G K++ Sbjct: 918759 ALLLRHYDSECVFV*EYLLRHYGDEQNIGTVSVKVD 918866 Database: P.ultimum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 42,801,618 Number of sequences in database: 82 Lambda K H 0.317 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 23,125,739 Number of Sequences: 82 Number of extensions: 353344 Number of successful extensions: 1509 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 1409 Number of HSP's gapped (non-prelim): 263 length of query: 488 length of database: 14,267,206 effective HSP length: 106 effective length of query: 382 effective length of database: 14,258,514 effective search space: 5446752348 effective search space used: 5446752348 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 64 (29.3 bits)