TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= TR1 #anophagefferens # scaffold_11:subseq(783554,7436).pep (488 letters) Database: P.pallidum/genome.fa 42 sequences; 32,942,533 total letters Searching..........................................done Score E Sequences producing significant alignments: (bits) Value gb|GL291012.1| Polysphondylium pallidum PN500 unplaced genomic s... 170 3e-47 gb|GL291013.1| Polysphondylium pallidum PN500 unplaced genomic s... 139 5e-33 gb|GL290984.1| Polysphondylium pallidum PN500 unplaced genomic s... 29 7.3 >gb|GL291012.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig15, whole genome shotgun sequence Length = 727357 Score = 170 bits (430), Expect(2) = 3e-47 Identities = 127/386 (32%), Positives = 187/386 (48%), Gaps = 4/386 (1%) Frame = -3 Query: 16 LACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLMHTASIIGETLXXXX 75 +A ++ AK G KV +++ + +GGTCVNVGC+PKK+M A+ I E L Sbjct: 504041 IATARRAAKYGVKVGLIEKTR---------MGGTCVNVGCVPKKVMWNAATIKEALHAAP 503889 Query: 76 XXXXXXXXXXXXHDWESMKSTVQDHIKSLNFKYRVALREAGITYLNRLGEFEDKNTISLT 135 H W ++K +I LN Y L + + +N G+F + + Sbjct: 503888 FYGFEGADKVTYH-WPTIKKNRDAYIARLNGIYNNMLAGSNVKSINGWGKFTGPRQLEVD 503712 Query: 136 DKKGNKTSVTAARFIVAVGGRPNKLGCPGGEHAIDSDDLFSLPKAPGRVLCVGGGYIALE 195 + TA ++A GG P PG E I SD F L + P V +G GYIA+E Sbjct: 503711 GQV-----YTADNILIATGGYPQIPQVPGAELGITSDGFFDLAELPKSVAVIGAGYIAVE 503547 Query: 196 CGGFTAGMGYPTTCMVR-SILLRGFDRECVGKIEAHIKHHGVQLQVGVTPAKIEKDAATG 254 G +G ++ ++R LR FD K++ + G+ + T + + K G Sbjct: 503546 LVGILNTLGSKSSLVIRHDHFLRTFDDIVAHKLQEQMVTDGINIVTNSTISALRK-TENG 503370 Query: 255 VLTVTDSGGGVHEYDTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVDE-QLPGA 313 + V + + DTV+ A GR T +G++ G S N K VDE Q GA Sbjct: 503369 QIVVVTNHTELPPVDTVIWAIGRDPSTKGIGLE-AAGVSVNERGYIK----VDEFQYTGA 503205 Query: 314 PHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTEPM--DYECVATTIFTPLEYGTIG 371 V+A+GDV G ELTPVAI AG RLA RLF K E + DYE + + +F+ GT+G Sbjct: 503204 QGVFAVGDVT-GNKELTPVAIAAGRRLAERLF-NKQEGLKFDYENIPSVVFSHPPIGTVG 503031 Query: 372 LSEDDAKAQLGECNVESYISEFAPLE 397 L+E +A A+ G+ NV+ Y S + L+ Sbjct: 503030 LTEKEAIAKYGKENVKVYNSRYVNLQ 502953 Score = 38.1 bits (87), Expect(2) = 3e-47 Identities = 20/60 (33%), Positives = 32/60 (53%) Frame = -1 Query: 389 YISEFAPLEYALSETRSERGDGCFAKLVVDKSTGKVVGFHYLGPNAGEITQGFSIAMRKG 448 Y F + YA+ + + F KL+V KVVG H +G + E+ QGF++A++ G Sbjct: 502888 YYCSFTNMYYAVVDDYKPK---TFMKLIVTGKEEKVVGIHSIGIGSDEMIQGFAVAIKMG 502718 >gb|GL291013.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig16, whole genome shotgun sequence Length = 228641 Score = 139 bits (350), Expect = 5e-33 Identities = 123/455 (27%), Positives = 199/455 (43%), Gaps = 9/455 (1%) Frame = -2 Query: 21 ECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLMHTASIIGETLXXXXXXXXX 80 + +LG KV V++ LGGTC+NVGCIP K + AS E Sbjct: 168982 KAGQLGMKVTVVE--------KRGKLGGTCLNVGCIPSKALLNASHKYEDAKTKFANYGV 168827 Query: 81 XXXXXXXHDWESMKSTVQDHIKSLNFKYRVALREAGITYLNRLGEFEDKNTISLTDKKGN 140 D +M + + L ++ +TY G+ NT+ +T + G Sbjct: 168826 KVGSVEL-DLAAMMKYKEKSVNGLTSGIEGLFKKNKVTYAKGHGKITGPNTVEVTGEDGK 168650 Query: 141 KTSVTAARFIVAVGGRPNKLGCPGGEHA--IDSDDLFSLPKAPGRVLCVGGGYIALECGG 198 ++T ++A G L + + S +L P R++ +GGG I LE G Sbjct: 168649 VQTITTKNIVIATGSEVASLPNVNIDEQTIVSSTGALALKAVPKRMVVIGGGVIGLELGS 168470 Query: 199 FTAGMGYPTTCM-VRSILLRGFDRECVGKIEAHIKHHGVQLQVGVTPAKIEKDAATGVLT 257 + +G TT + + + G D E K + ++ ++ + + K+A G +T Sbjct: 168469 VWSRLGAETTVVEFTNRIAAGADGEVAKKFQKVLEKQHMKFHLETKVTSVVKNA-NGTVT 168293 Query: 258 VT----DSGG--GVHEYDTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVDEQLP 311 VT +GG G E D VLV+ GR +T LG+D V G + K ++E D Sbjct: 168292 VTVESVGAGGFSGQIEADVVLVSVGRRPNTTNLGLDKV-GVPMD---KAGRVEVGDHFKT 168125 Query: 312 GAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTEPMDYECVATTIFTPLEYGTIG 371 +YAIGD ++G P L A E G+ + L G ++Y + + I+T E +G Sbjct: 168124 NVKSIYAIGDAIKG-PMLAHKAEEEGIAVMEYLHNGSGH-VNYAAIPSVIYTHPEVAWVG 167951 Query: 372 LSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTGKVVGFHYLG 431 +E++ + + NV + +A A + SE F K + D ST +++G H +G Sbjct: 167950 KTEEELTKEGIKFNVGKF--PYAANSRARTNDESEG----FVKFLSDSSTDRILGVHIMG 167789 Query: 432 PNAGEITQGFSIAMRKGATYADFISTVGIHPTVAE 466 AGE+ +AM GA+ D T HPT++E Sbjct: 167788 DCAGEMIAESVLAMEYGASSEDVARTCHAHPTLSE 167684 >gb|GL290984.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold2, whole genome shotgun sequence Length = 3242543 Score = 29.3 bits (64), Expect = 7.3 Identities = 15/56 (26%), Positives = 30/56 (53%), Gaps = 2/56 (3%) Frame = -1 Query: 427 FHYLGPNAGEITQGFSIAMRKGATYAD--FISTVGIHPTVAEEFTSMTVTKSSGES 480 +H L P +++G SI + G + F+ ++ ++PT + + ++ T S GES Sbjct: 3105269 YHNLAPT---LSRGESITLNNGQSMTQQIFLKSIELYPTNSNSYHNLATTLSRGES 3105111 Database: P.pallidum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 32,942,533 Number of sequences in database: 42 Lambda K H 0.317 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,017,395 Number of Sequences: 42 Number of extensions: 153682 Number of successful extensions: 518 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 495 Number of HSP's gapped (non-prelim): 59 length of query: 488 length of database: 10,980,844 effective HSP length: 104 effective length of query: 384 effective length of database: 10,976,476 effective search space: 4214966784 effective search space used: 4214966784 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 63 (28.9 bits)