TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= TR1 #anophagefferens # scaffold_11:subseq(783554,7436).pep (488 letters) Database: N.gruberi/genome.fa 784 sequences; 40,964,085 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG738881.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 326 4e-89 gb|GG738854.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 193 2e-85 gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 305 5e-83 gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 120 3e-27 gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 40 0.004 gb|GG738887.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 33 0.62 gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 31 3.1 >gb|GG738881.1| Naegleria gruberi genomic scaffold NAEGRscaffold_37, whole genome shotgun sequence Length = 379849 Score = 326 bits (835), Expect = 4e-89 Identities = 198/449 (44%), Positives = 252/449 (56%), Gaps = 13/449 (2%) Frame = -3 Query: 53 VGCIPKKLMHTASIIGE-----TLXXXXXXXXXXXXXXXXHDWESMKSTVQDHIKSLNFK 107 VGCIPKKLMHTA++IG+ T H W + VQDHI LNFK Sbjct: 49346 VGCIPKKLMHTAALIGDRSQDGTAFGWTNSVGHGTERKPHHQWGKLVEMVQDHIHGLNFK 49167 Query: 108 YRVALREAGITYLNRLGEFEDKNTIS-LTDKKGNKTSVTAARFIVAVGGRPNKLGCPGG- 165 YR LR+ GITY N F D +T+ D + TA RF +AVGGRP PG Sbjct: 49166 YRTDLRKKGITYHNLYASFIDAHTVEGKKDGQEEGKRFTARRFAIAVGGRPLYPDIPGAK 48987 Query: 166 EHAIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYPTTCMVRSILLRGFDRECVG 225 E+ I SDD+FS+ K PG+ L VG Y+ALECGGF G+G TT MVRS+ LRGFDREC Sbjct: 48986 EYCISSDDIFSMEKEPGKSLVVGASYVALECGGFLHGIGNDTTIMVRSVPLRGFDRECAD 48807 Query: 226 KIEAHIKHHGVQLQVGVTPAKIEKDAATGVLTVTDSG-----GGVHEYDTVLVATGRYAD 280 KI + GV+ P + K +G L+V E+DTVL ATGRYA Sbjct: 48806 KIVNVMSDEGVKFYQPCVPLSVNK-LESGKLSVEYENVETKEKKTEEFDTVLFATGRYAL 48630 Query: 281 TAALGIDNVPGASANVNAKTKKLECVDEQLPGAPHVYAIGDVVEGRPELTPVAIEAGLRL 340 T AL ++N+ G + K K+ + PH+YAIGDV+ G ELTPVAI+AG L Sbjct: 48629 TQALNLENI-GVKTD---KVGKIFVNKYEQSSVPHIYAIGDVIVGGLELTPVAIKAGKLL 48462 Query: 341 ARRLFGGKTEPMDYECVATTIFTPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYAL 400 A RL+ + MDY+ V TT+FTP+EYG+ GL+E++A + G N+ Y + LEY Sbjct: 48461 AARLYNSSKKMMDYDKVPTTVFTPVEYGSCGLTEEEAVKRYGAENLTIYKKSYYVLEYEP 48282 Query: 401 SETRSERGDGCFAKLVVDK-STGKVVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVG 459 + F KL+ ++ +V+GFHY+GPNAGE+TQGF++AMR AT DF TVG Sbjct: 48281 PARECQ----AFVKLICNELDNERVLGFHYVGPNAGEVTQGFAVAMRCNATKEDFDDTVG 48114 Query: 460 IHPTVAEEFTSMTVTKSSGESAAKGGCUG 488 IHPT AE T+ K GC G Sbjct: 48113 IHPTCAE-----TIVKLEKGVETDTGC*G 48042 Score = 68.2 bits (165), Expect = 2e-11 Identities = 30/52 (57%), Positives = 36/52 (69%) Frame = -3 Query: 1 GFDYDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVN 52 G+DYD +A +KE LGAKVAV D++KPSP G+TWGLGGTCVN Sbjct: 49712 GYDYDLIVIGGGSGGIALAKEANALGAKVAVFDYIKPSPQGTTWGLGGTCVN 49557 >gb|GG738854.1| Naegleria gruberi genomic scaffold NAEGRscaffold_10, whole genome shotgun sequence Length = 665159 Score = 193 bits (490), Expect(3) = 2e-85 Identities = 129/348 (37%), Positives = 178/348 (51%), Gaps = 40/348 (11%) Frame = -2 Query: 16 LACSKECAKLGAK---VAVLDFVKPSPA----------GSTWGLGGTCVNVGCIPKKLMH 62 L+ SK+ + L +K + +LD+ PS G W LGGTCVNVGCIPKKLMH Sbjct: 89416 LSLSKQLSSLSSKETRICLLDYRDPSSQWLRERNGKLNGWMWQLGGTCVNVGCIPKKLMH 89237 Query: 63 TASIIGETLXXXXXXXXXXXXXXXX-----HDWESMKSTVQDHIKSLNFKYRVALREA-- 115 +++IGE + HDW+++ + VQ +IKSLNF YR ++ + Sbjct: 89236 NSALIGEGINHDAEYFGWNDGKQEGKLSGKHDWKTLVANVQQYIKSLNFGYRTSVTQTYA 89057 Query: 116 ---------GITYLNRLGEFEDKNTISLTD-KKGNKTSVTAARFIVAVGGRPN-KLGCPG 164 + YLN G F D +T+ LTD +KG +++ I++VGG PN PG Sbjct: 89056 FDNEDPSLKKLYYLNMKGRFVDDHTLELTDPRKGITKTISGKVIILSVGGTPNIPQDVPG 88877 Query: 165 G-EHAIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYPTTCMVRSILLRGFDREC 223 E+AI SDD+FSL + PG+ L +G YIALE F G+GY ++ M+RSI LRGFD+EC Sbjct: 88876 ATEYAITSDDIFSLKEEPGKTLVIGASYIALETASFLKGLGYESSVMMRSIPLRGFDQEC 88697 Query: 224 VGKIEAHIKHHGVQLQVGVTPAKIEKDA---ATGVLTV-----TDSGGGVHEYDTVLVAT 275 K+ +K GV+ P KIEK + TG TV + Y T++ A Sbjct: 88696 AWKVVDGMKERGVKFLEQCIPVKIEKSSDHETTGEYTVHYKDLSTQQVYTENYKTIMFAV 88517 Query: 276 GRYADTAALGIDNVPGASANVNAKTKKLECVDEQLPGAPHVYAIGDVV 323 GR A T L ID S K+ D + H+YAIGD + Sbjct: 88516 GRRAVTKELNIDLDMDESG-------KIIVSDNEQTSKKHIYAIGDCI 88394 Score = 110 bits (276), Expect(3) = 2e-85 Identities = 62/123 (50%), Positives = 84/123 (68%), Gaps = 2/123 (1%) Frame = -1 Query: 326 RPELTPVAIEAGLRLARRLFGGKTEPMDYECVATTIFT-PLEYGTIGLSEDDAKAQLGEC 384 + ELTPVAI AG LA RL TE MDY+ V T+IFT P EYG GLSE++A + GE Sbjct: 88343 KTELTPVAIRAGKLLASRLTNKGTELMDYDNVPTSIFTHPYEYGCCGLSEEEAVQRYGED 88164 Query: 385 NVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTG-KVVGFHYLGPNAGEITQGFSI 443 ++E YIS ++P+E+ LS + + F K++ KS KV+GFHY+G NAGEITQG ++ Sbjct: 88163 DIEVYISLYSPIEHQLSHRDTNK---TFMKIITLKSANEKVIGFHYVGANAGEITQGIAV 87993 Query: 444 AMR 446 A++ Sbjct: 87992 AIK 87984 Score = 53.5 bits (127), Expect(3) = 2e-85 Identities = 25/43 (58%), Positives = 30/43 (69%) Frame = -3 Query: 446 RKGATYADFISTVGIHPTVAEEFTSMTVTKSSGESAAKGGCUG 488 R GAT F +T+GIHPT AEE T +++TK SG SA K GC G Sbjct: 87933 RAGATKEHFDNTIGIHPTAAEEMTLLSITKRSGNSAEKDGC*G 87805 >gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_8, whole genome shotgun sequence Length = 938756 Score = 305 bits (782), Expect = 5e-83 Identities = 205/526 (38%), Positives = 267/526 (50%), Gaps = 61/526 (11%) Frame = +1 Query: 4 YDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVN----------- 52 YD L CSK A LGAKVA+LD+VKPS GSTW +GGTCVN Sbjct: 731830 YDLIVVGGGSGGLKCSKLAADLGAKVALLDYVKPSSQGSTWKIGGTCVNGM*WID*LRWS 732009 Query: 53 ------------------VGCIPKKLMHTASI------------------IGETLXXXXX 76 VGCIPKK MH AS +GE Sbjct: 732010 TNDFNILYMHVCS*LK*SVGCIPKKQMHFASEMGNKIREAINYGWKLPLGLGEAPEEDMF 732189 Query: 77 XXXXXXXXXXXHDWESMKSTVQDHIKSLNFKYRVALREAGITYLNRLGEFEDKNTISLTD 136 DW ++ V + NF Y+ LR+ I + LG D +T+ L Sbjct: 732190 NNFPHNKRGEFFDWTTLVQGVLNTRSGSNFVYKGELRKRKIDFYEALGRLVDSHTVELV- 732366 Query: 137 KKGNKTSVTAARFIVAVGGRPN-KLGCPGG-EHAIDSDDLFSLPKAPGRVLCVGGGYIAL 194 G +TA ++A GGRPN PG E+AI SDDLFSL +APG+ L VGG YIAL Sbjct: 732367 --GRVERLTAKYILLAPGGRPNVPRDIPGAYEYAITSDDLFSLQQAPGKTLVVGGSYIAL 732540 Query: 195 ECGGFTAGMGYPTTCMVRSILLR--GFDRECVGKIEAHIK-HHGVQLQVGVTPAKIEK-- 249 EC GF +G+G+ + MVRS +LR FD E + ++ HH V+ P KIEK Sbjct: 732541 ECAGFLSGLGFDVSVMVRSRILRSEAFDAEIASILRESMELHHHVKFLEPCIPVKIEKLE 732720 Query: 250 --DAATGVLTVTDSGGGVHEYDTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVD 307 L T++ ++DTV+ ATGRYAD L + A + K K+ D Sbjct: 732721 NNQKRVTFLNATENKEYTQDFDTVMFATGRYADVQGLNL----AAIGVQHTKEGKIIVND 732888 Query: 308 EQLPGAPHVYAIGDVVEG--RPELTPVAIEAGLRLARRLFGGK--TEPMDYECVATTIFT 363 E+ +YAIGDV+E ELTPVAI+ G LA RLF + + +DY+ V TT+FT Sbjct: 732889 EERTSVESIYAIGDVIENGHNYELTPVAIQQGKYLAYRLFKPEEINKKVDYDFVPTTVFT 733068 Query: 364 PLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLV-VDKSTG 422 P EYG +G SE+ AK GE N+ Y +F LE+ ++E G+ F KL+ V Sbjct: 733069 PTEYGLVGYSEEKAKKVFGENNLVIYKKKFNILEHKIAEI----GEKGFVKLICVKNQNE 733236 Query: 423 KVVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEF 468 +VVG HYLGPNA E+TQGF++A++KG T +F +GIHP+ AE F Sbjct: 733237 RVVGLHYLGPNAAEVTQGFALALKKGCTKEEFDDVIGIHPSNAEAF 733374 >gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_9, whole genome shotgun sequence Length = 695587 Score = 120 bits (301), Expect = 3e-27 Identities = 105/400 (26%), Positives = 182/400 (45%), Gaps = 9/400 (2%) Frame = +3 Query: 89 DWESMKSTVQDHIKSLNFKYRVALREAGITYLNRLGEFEDKNTISLTDKKGNKTSVTAAR 148 D M+ +K L ++ + Y+ G KN +S+ G +++ Sbjct: 643617 DVSVMQKQKDKAVKGLTGGIEFLFKKYKVEYVKGEGTVTGKNEVSVKGLDGKNSTIATDN 643796 Query: 149 FIVAVGGRPNKLG-CPGGEHAI-DSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYP 206 ++A G L P E I S +L P R+ +G G I LE G + +G Sbjct: 643797 IVIATGSEATPLPFLPFDEKVILSSTGALALDHVPKRMTVIGAGVIGLEMGSVYSRLGSE 643976 Query: 207 TTCMVRSILLRGF-DRECVGKIEAHIKHHGVQLQVGVTPAKIEK--DAATGVLTVTDSGG 263 T + S + F D+E + ++ G++ ++GV+ K K D T L + ++ Sbjct: 643977 VTVIEYSDRISPFLDKEVSAVFQKTMEKLGMKFKLGVS-VKSGKVVDGKTCQLELVNNAT 644153 Query: 264 GV---HEYDTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVDEQL-PGAPHVYAI 319 G E D LV+ GR TA LG++NV G + + + VD+ ++YAI Sbjct: 644154 GAAETFESDVCLVSIGRRPHTANLGLNNV-GITLDERGRVP----VDDHFRTSVSNIYAI 644318 Query: 320 GDVVEGRPELTPVAIEAGLRLARRLFGGKTEPMDYECVATTIFTPLEYGTIGLSEDDAKA 379 GDVV G P L A + G+ +A +L G++ ++Y+ + I+T E ++G +E++ Sbjct: 644319 GDVVRG-PMLAHKAEDEGIAIAEQL-AGRSGHVNYDAIPNVIYTHPEIASVGKTEEE--- 644483 Query: 380 QLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTGKVVGFHYLGPNAGEITQ 439 L + ++ + +F + + T + +G F K++ D T KV+G H +G AGE+ Sbjct: 644484 -LVKAGIKYKVGKFPYSANSRARTIDDGTEG-FVKILADAQTDKVLGVHIIGIYAGELIA 644657 Query: 440 GFSIAMRKGATYADFISTVGIHPTVAEEFTSMTVTKSSGE 479 ++AM GA+ D T HPT++E V G+ Sbjct: 644658 EATLAMEYGASSEDIARTCHAHPTLSEAIKEAAVAAYDGK 644777 >gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_62, whole genome shotgun sequence Length = 286411 Score = 40.4 bits (93), Expect = 0.004 Identities = 43/147 (29%), Positives = 61/147 (41%), Gaps = 8/147 (5%) Frame = +3 Query: 184 VLCVGGGYIALECGGFTAGMGYPTTCMVRSILLRGFDRECVGKIE-AHIKHHGVQLQVGV 242 ++ +G GYIA E F A G T VRS L+ D E + + K + + G+ Sbjct: 181323 LIVIGAGYIATELAHFFASTGTKTNIFVRSGFLKHEDEEVQKEFDRVFSKKKNINVIKGM 181502 Query: 243 TPAKIEKDAATGVLTV---TDSGGGVHEY--DTVLVATGRYADTAALGIDNVPGASANVN 297 K + VLT +D G E + +LVA G +T L D A + Sbjct: 181503 KILKARYENDEFVLTYSNPSDESSGTIEVRAEGLLVAAGVVPNTDILQCD-----KAGIQ 181667 Query: 298 AKTKKLECVDEQLP--GAPHVYAIGDV 322 VD+ L AP+V+A GDV Sbjct: 181668 TSKDGFIIVDKFLKSVNAPNVWAFGDV 181748 >gb|GG738887.1| Naegleria gruberi genomic scaffold NAEGRscaffold_43, whole genome shotgun sequence Length = 508457 Score = 33.1 bits (74), Expect = 0.62 Identities = 24/92 (26%), Positives = 43/92 (46%), Gaps = 6/92 (6%) Frame = +1 Query: 322 VVEGRPELTPVAIEAGLRLARRLFGGKTEPMDYECVATTIFTPLEYGTIGLSE------D 375 VVE + E TPV +E + + K E E +P+E TI + E + Sbjct: 18259 VVEDKKEETPVVVEETQPVVETVVEKKEEETPIESTPAVTESPVE--TIIVEETQPVVVE 18432 Query: 376 DAKAQLGECNVESYISEFAPLEYALSETRSER 407 + K ++ NVE+ ++E P++ ++E E+ Sbjct: 18433 EKKEEIVSENVETVVAETTPVQQEVTEVSEEK 18528 >gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_3, whole genome shotgun sequence Length = 941567 Score = 30.8 bits (68), Expect = 3.1 Identities = 16/30 (53%), Positives = 21/30 (70%), Gaps = 1/30 (3%) Frame = -2 Query: 349 TEPMDYECVATTIFTP-LEYGTIGLSEDDA 377 T+ MDY+ V T+IF EYG GLSE++A Sbjct: 100432 TKLMDYDNVTTSIFNH*YEYGCCGLSEEEA 100343 Database: N.gruberi/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 40,964,085 Number of sequences in database: 784 Lambda K H 0.317 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,219,169 Number of Sequences: 784 Number of extensions: 209179 Number of successful extensions: 673 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 561 Number of HSP's gapped (non-prelim): 193 length of query: 488 length of database: 13,654,695 effective HSP length: 106 effective length of query: 382 effective length of database: 13,571,591 effective search space: 5184347762 effective search space used: 5184347762 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 64 (29.3 bits)