TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= TR1 #anophagefferens # scaffold_11:subseq(783554,7436).pep (488 letters) Database: C.owczarzaki/genome.fa 89 sequences; 28,043,798 total letters Searching........................................................................................done Score E Sequences producing significant alignments: (bits) Value supercontig_1.2 of Capsaspora owczarzaki ATCC 30864 300 1e-88 supercontig_1.13 of Capsaspora owczarzaki ATCC 30864 89 9e-18 supercontig_1.4 of Capsaspora owczarzaki ATCC 30864 30 2.8 >supercontig_1.2 of Capsaspora owczarzaki ATCC 30864 Length = 2668053 Score = 300 bits (768), Expect(2) = 1e-88 Identities = 181/426 (42%), Positives = 237/426 (55%), Gaps = 61/426 (14%) Frame = +3 Query: 88 HDWESMKSTVQDHIKSLNFKYRVALREAGITYLNRLGEFEDKNTISLTDKKGNKTSVTAA 147 HDW + V DHI LNF YRV+LRE +TYLN G F+D +TI TDKK T++TA Sbjct: 1734957 HDWSKLVMGVSDHIGGLNFAYRVSLRENNVTYLNAYGVFKDAHTIECTDKKRKVTTLTAE 1735136 Query: 148 RFIVAVGGRPNKLGCPGG-EHAIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYP 206 F++AVGGRP PG E I SDD+FSLP APG+ L VG Y+ALEC GF G+GY Sbjct: 1735137 HFVLAVGGRPKYPDIPGARELGITSDDMFSLPYAPGKTLVVGASYVALECAGFLTGLGYD 1735316 Query: 207 TTCMVRSILLRGFDRECVGKIEAHIKHHGVQLQVGVTPAKIEK-----DAATG--VLTVT 259 TT M RSI LRGFD++C +I A++ HG + P +E+ D+ATG + VT Sbjct: 1735317 TTVMARSIFLRGFDQQCAEQIAAYMSEHGTKFLRRCVPLSLERIEPEADSATGRHRIRVT 1735496 Query: 260 DSGGGVHE-----YDTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVDEQL--PG 312 G +E +DTVL A GR A T+ +G++ + G AN + K VD L Sbjct: 1735497 WEDGLANEPGSDVFDTVLFAIGREAVTSTIGLEAL-GLKANASGK------VDAPLEQTS 1735655 Query: 313 APHVYAIGDVVEGRPELTPVAIE------------------------------------- 335 P+V+AIGDV+ GRPELTPVAI+ Sbjct: 1735656 VPNVFAIGDVINGRPELTPVAIQVRVWSSSFAREGLLHSGLCCRGLIPSLPLPRLLPIII 1735835 Query: 336 --------AGLRLARRLFGGKTEPMDYECVATTIFTPLEYGTIGLSEDDAKAQLGECNVE 387 AG LA+RL+GG T MDY + TT+FTPLEYG IG++E+DA A E N+E Sbjct: 1735836 IFSHDDA*AGRLLAKRLYGGATTQMDYANIPTTVFTPLEYGCIGMAEEDAIALYKEENIE 1736015 Query: 388 SYISEFAPLEYALSETRSERGDGCFAKLVVDK-STGKVVGFHYLGPNAGEITQGFSIAMR 446 + F PLE+ ++ + C+ KL+ +K +V+G H LGPNAGEITQG+ +A R Sbjct: 1736016 VFHQYFQPLEWTVAHRPAST---CYCKLICNKLDNMRVIGLHILGPNAGEITQGYGVAFR 1736186 Query: 447 KGATYA 452 +A Sbjct: 1736187 *DGCFA 1736204 Score = 144 bits (363), Expect = 1e-34 Identities = 106/343 (30%), Positives = 166/343 (48%), Gaps = 34/343 (9%) Frame = -2 Query: 163 PGGEHAIDSDDLFSLPKAPGRVLCVGGGY------------------------------- 191 PG H I SD F L + P +V VG GY Sbjct: 397787 PGAHHGITSDGFFELNEQPKKVAVVGAGYVPVFFFGGGLHYLKFEQSVRTDIQMRHT*SR 397608 Query: 192 -IALECGGFTAGMGYPTTCMVRSI-LLRGFDRECVGKIEAHIKHHGVQLQVGVTPAKIEK 249 IA+E G +G + +VR +LR FD +E + GV L + + + K Sbjct: 397607 YIAVELAGIFRALGSEVSLLVRHDGVLRKFDSMLHKGVEEELTASGVNLLLNTHVSSVTK 397428 Query: 250 DAATGVLTVTDSGGGVHEYDTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVDEQ 309 D + + +G +D ++ A GR L ++ G S + + K E + Sbjct: 397427 DDSGHLSLALTNGTTQSGFDCLVWAIGRSPTIEDLDLEKT-GVSLDKDGLIKVDEFQNTT 397251 Query: 310 LPGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTE-PMDYECVATTIFTPLEYG 368 G ++A+GDV +LTPVAI AG RL+ RLF GKT+ ++YE +A+ +F+ G Sbjct: 397250 HEG---IHAVGDVAS-HWQLTPVAIAAGRRLSERLFAGKTDLKLEYENIASVVFSHPALG 397083 Query: 369 TIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTGKVVGFH 428 T+GLSE+DA A+ G+ N++ Y + F + ++++ +++ KLV KV+G H Sbjct: 397082 TVGLSEEDAIAKHGKDNLKIYSATFTNMYHSMTTRKTKTR----VKLVCLGKEEKVIGVH 396915 Query: 429 YLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEEFTSM 471 +G A E+ QGF++A++ GAT A F TV IHPT +EE ++ Sbjct: 396914 VIGIGADEMIQGFAVAVKMGATKAQFDDTVAIHPTASEELVTL 396786 Score = 90.5 bits (223), Expect = 2e-18 Identities = 41/55 (74%), Positives = 46/55 (83%) Frame = +3 Query: 17 ACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLMHTASIIGETL 71 A +K AKLG +V VLDFVKPSP GSTWGLGGTCVNVGCIPKKLMH A ++GE + Sbjct: 1734588 AMNK*AAKLGKRVCVLDFVKPSPQGSTWGLGGTCVNVGCIPKKLMHQACLLGEAI 1734752 Score = 45.8 bits (107), Expect(2) = 1e-88 Identities = 21/41 (51%), Positives = 27/41 (65%) Frame = +2 Query: 448 GATYADFISTVGIHPTVAEEFTSMTVTKSSGESAAKGGCUG 488 GAT DF TVGIHPT +E FT++ +T++SG GC G Sbjct: 1736276 GATKDDFDMTVGIHPTCSENFTTLDITRASGLDPGAKGC*G 1736398 Score = 28.9 bits (63), Expect = 8.1 Identities = 24/83 (28%), Positives = 36/83 (43%) Frame = -2 Query: 366 EYGTIGLSEDDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTGKVV 425 E TIG +E + + E S+ P + TR+E DGC ++ VDK G +V Sbjct: 1980380 ERSTIGRAEKREREKK-----EVKTSQARPSHHETRSTRAEEEDGCVIEVKVDKVLG-LV 1980219 Query: 426 GFHYLGPNAGEITQGFSIAMRKG 448 G A + G + + KG Sbjct: 1980218 GDERAERAADDAVPGRVVLLVKG 1980150 >supercontig_1.13 of Capsaspora owczarzaki ATCC 30864 Length = 955311 Score = 88.6 bits (218), Expect = 9e-18 Identities = 86/332 (25%), Positives = 146/332 (43%), Gaps = 11/332 (3%) Frame = -1 Query: 112 LREAGITYLNRLGEFEDKNTISLTDKKGNKTSVTAARFIVAVGGRPNKLGCPG----GEH 167 L++ + Y+ G+ +++ G + A R ++A G PG + Sbjct: 534672 LKKNKVDYVKGHGKITGAQEVTVDLLGGGSQVLKAKRILIATGSEVTPF--PGIAIDEQT 534499 Query: 168 AIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYPTTCM--VRSILLRGFDRECVG 225 + S +L P +++ +GGG I LE G + +G T + + I G D E Sbjct: 534498 IVSSTGALALKAVPKKMIVIGGGVIGLELGSVWSRLGAEVTVVEFLGGIGGAGIDGEVAK 534319 Query: 226 KIEAHIKHHGVQLQVGVTPAKIEKDAATGV-LTVTDSGGGVHEY---DTVLVATGRYADT 281 + + G+ ++ K A V + V + G V E D +LVA GR T Sbjct: 534318 NFQRVLTKQGLNFKLNTKVTGATKQANGSVKVNVESADGSVKETLDADVLLVAIGRRPYT 534139 Query: 282 AALGIDNVPGASANVNAKTK-KLECVDEQLPGAPHVYAIGDVVEGRPELTPVAIEAGLRL 340 LG+ A V + ++E + P +YAIGDV++G P L A + G+ Sbjct: 534138 NNLGLKE-----AGVKVDNRGRVEVNNMFTTNVPSIYAIGDVIQG-PMLAHKAEDEGILA 533977 Query: 341 ARRLFGGKTEPMDYECVATTIFTPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLEYAL 400 + GG +DY V + I+T E +G +E+ KA VE I +F + + Sbjct: 533976 VEGMCGGHPH-IDYNNVPSVIYTHPEVAWVGKTEEQLKAD----GVEYTIGKFPFVANSR 533812 Query: 401 SETRSERGDGCFAKLVVDKSTGKVVGFHYLGP 432 ++T ++ DG F K++ DK T +++G H +GP Sbjct: 533811 AKTNNDT-DG-FVKVLGDKKTDQILGAHIIGP 533722 Score = 33.9 bits (76), Expect = 0.25 Identities = 18/43 (41%), Positives = 26/43 (60%) Frame = -3 Query: 234 HGVQLQVGVTPAKIEKDAATGVLTVTDSGGGVHEYDTVLVATG 276 HG++L +GV+ ++ DA T L S G V +YD +VATG Sbjct: 505111 HGIELLLGVSVTELAADAKTATL----SDGQVLKYDFAIVATG 504995 Score = 32.7 bits (73), Expect = 0.56 Identities = 12/16 (75%), Positives = 14/16 (87%) Frame = -3 Query: 46 LGGTCVNVGCIPKKLM 61 LGGTC+NVGCIP K + Sbjct: 534988 LGGTCLNVGCIPSKAL 534941 >supercontig_1.4 of Capsaspora owczarzaki ATCC 30864 Length = 2141673 Score = 30.4 bits (67), Expect = 2.8 Identities = 15/30 (50%), Positives = 17/30 (56%) Frame = +1 Query: 308 EQLPGAPHVYAIGDVVEGRPELTPVAIEAG 337 EQ P HV + VVEGRP LT + I G Sbjct: 1820836 EQQPNREHVATMCSVVEGRPSLTILRINQG 1820925 Database: C.owczarzaki/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 28,043,798 Number of sequences in database: 89 Lambda K H 0.317 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,754,451 Number of Sequences: 89 Number of extensions: 248894 Number of successful extensions: 1201 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 1011 Number of HSP's gapped (non-prelim): 343 length of query: 488 length of database: 9,347,932 effective HSP length: 103 effective length of query: 385 effective length of database: 9,338,765 effective search space: 3595424525 effective search space used: 3595424525 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 62 (28.5 bits)