TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= TR1 #anophagefferens # scaffold_11:subseq(783554,7436).pep (488 letters) Database: C.muris/genome.fa 84 sequences; 9,245,251 total letters Searching...................................................................................done Score E Sequences producing significant alignments: (bits) Value gb|DS989740.1| Cryptosporidium muris RN66 scf_1106632353993 geno... 406 e-114 gb|DS989727.1| Cryptosporidium muris RN66 scf_1106632353999 geno... 70 1e-12 >gb|DS989740.1| Cryptosporidium muris RN66 scf_1106632353993 genomic scaffold, whole genome shotgun sequence Length = 196818 Score = 406 bits (1043), Expect = e-114 Identities = 229/502 (45%), Positives = 302/502 (60%), Gaps = 15/502 (2%) Frame = +1 Query: 2 FDYDXXXXXXXXXXLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLM 61 F YD LA +KE A+LG KVA+ D+VKPS G+ WGLGGTCVNVGC+PKKL+ Sbjct: 13348 FMYDLIVIGGGSGGLAAAKEAARLGKKVALFDYVKPSTQGTKWGLGGTCVNVGCVPKKLI 13527 Query: 62 HTASIIGETLXXXXXXXXXXXXXXXXHDWESMKSTVQDHIKSLNFKYRVALREAGITYLN 121 H A+ IG L W ++ TVQ+H+KSLNF YR L +G+ Y+N Sbjct: 13528 HYAANIGTILHHDTSNYGFEVTSTFR--WSNLIQTVQNHVKSLNFSYRSGLFSSGVEYIN 13701 Query: 122 RLGEFEDKNTISLTDKKGNKTSVTAARFIVAVGGRPN-KLGCPGG-EHAIDSDDLFSLPK 179 L ED++T+ L + T ++AVGGRPN PG E+AI SDD+FSL K Sbjct: 13702 SLANLEDRHTV-LFKRNNEIMKYTTEHILIAVGGRPNISESVPGALEYAITSDDIFSLSK 13878 Query: 180 APGRVLCVGGGYIALECGGFTAGMGYPTTCMVRSILLRGFDRECVGKIEAHIKHHGVQLQ 239 PG+ L VG YI LE GF +G+ VRSI LRGFDR+C K+ + +K +G + Sbjct: 13879 PPGKTLIVGASYIGLETAGFLTELGFEAIVAVRSIPLRGFDRQCSEKVVSMMKEYGTRFL 14058 Query: 240 VGVTPAKIEKDAATGVLTVTDSGGGVHEYDTVLVATGRYADTAALGIDNVPGASANVNAK 299 GV P IEK L V S G ++DTV+ ATGRY DT L ++ + V+ Sbjct: 14059 EGVVPINIEK--IDDELKVVFSNGYTEQFDTVIYATGRYPDTKYLKLNEI-----GVHLT 14217 Query: 300 TKKLECVDEQLPGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTEPMDYECVAT 359 + P+++AIGDVV+GRPELTPVAI+AG+ LARRLFG E +DY+ + T Sbjct: 14218 SNNKIIAPNDTTNIPNIHAIGDVVDGRPELTPVAIKAGILLARRLFGNSKEFIDYKYIPT 14397 Query: 360 TIFTPLEYGTIGLSEDDAKAQLGECNVESYISEFAPLE------YALSETRSERGD---- 409 TIFTP+EYG+IG S + A A+ GE ++E Y+ EF LE Y + +R + GD Sbjct: 14398 TIFTPIEYGSIGYSSEAAIAEYGEDDIEEYLFEFTTLEIAASHRYKVESSRMDSGDFYMS 14577 Query: 410 -GCFAKLVVDKS-TGKVVGFHYLGPNAGEITQGFSIAMRKGATYADFISTVGIHPTVAEE 467 FAKL+ KS KV+GFH++G NAGEITQGFS+A++ G +DF S +GIHPT AE Sbjct: 14578 PNSFAKLITVKSLDNKVIGFHFVGLNAGEITQGFSLAIKLGTKKSDFDSMIGIHPTDAEV 14757 Query: 468 FTSMTVTKSSGES-AAKGGCUG 488 F+ +++T+ SGES A GGC G Sbjct: 14758 FSDLSITRRSGESFIATGGCGG 14823 >gb|DS989727.1| Cryptosporidium muris RN66 scf_1106632353999 genomic scaffold, whole genome shotgun sequence Length = 1306717 Score = 70.1 bits (170), Expect = 1e-12 Identities = 61/216 (28%), Positives = 98/216 (45%), Gaps = 4/216 (1%) Frame = +2 Query: 269 DTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVDEQ----LPGAPHVYAIGDVVE 324 D VL++ GR +T LG++N+ VN + +D + +P + ++YAIGDV+ Sbjct: 199091 DKVLLSVGRKPNTKNLGLENI-----GVNVDRRGYIPIDHETYRVIPYS-NIYAIGDVIG 199252 Query: 325 GRPELTPVAIEAGLRLARRLFGGKTEPMDYECVATTIFTPLEYGTIGLSEDDAKAQLGEC 384 G+ L A E G+ +A + GK P+ Y+ + T I+T E +G SE D L + Sbjct: 199253 GQM-LAHKAEEEGIFVAEFIANGKQSPIHYDAIPTVIYTHPEIACVGFSEKDLI--LKQI 199423 Query: 385 NVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTGKVVGFHYLGPNAGEITQGFSIA 444 + I A S R F K+ T +++G + NAGEI ++A Sbjct: 199424 PFKKGIFRMN----ANSRARVSGDVEGFVKVFEHSETKEILGSCIISANAGEIIHELALA 199591 Query: 445 MRKGATYADFISTVGIHPTVAEEFTSMTVTKSSGES 480 R A+ D HPT++E + S G + Sbjct: 199592 YRYRASCEDIARLCHAHPTLSEAIKEACWSVSFGSA 199699 Score = 32.3 bits (72), Expect = 0.25 Identities = 12/21 (57%), Positives = 16/21 (76%) Frame = +1 Query: 46 LGGTCVNVGCIPKKLMHTASI 66 +GGTC+N GCIP K + AS+ Sbjct: 197671 IGGTCLNEGCIPSKTLLNASL 197733 Score = 28.9 bits (63), Expect = 2.7 Identities = 18/68 (26%), Positives = 36/68 (52%), Gaps = 1/68 (1%) Frame = +1 Query: 256 LTVTDSGGGVHEYDTVLVATGRYADTAALGIDNVPGASANVNAKTKKLECVDEQL-PGAP 314 L + D ++ Y +LV++ Y++ + G+DN+ ++N K EC+DE + G Sbjct: 1170466 LNIMDIEPSLNSYSRILVSS--YSEYSN-GLDNLLTLGISINGKNFTRECLDEWINDGIC 1170636 Query: 315 HVYAIGDV 322 ++ I D+ Sbjct: 1170637 DIFCIKDI 1170660 Database: C.muris/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 9,245,251 Number of sequences in database: 84 Lambda K H 0.317 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,315,175 Number of Sequences: 84 Number of extensions: 36149 Number of successful extensions: 110 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 96 Number of HSP's gapped (non-prelim): 11 length of query: 488 length of database: 3,081,750 effective HSP length: 96 effective length of query: 392 effective length of database: 3,073,686 effective search space: 1204884912 effective search space used: 1204884912 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 58 (26.9 bits)