TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000036_1.0 # Protein # Thioredoxin reductase 3 (TR3) # Homo sapiens # Complete (754 letters) Database: C.parvum/genome.fa 8 sequences; 9,102,324 total letters Searching........done Score E Sequences producing significant alignments: (bits) Value gb|CM000430.1| Cryptosporidium parvum Iowa II chromosome 2, whol... 394 e-110 gb|CM000436.1| Cryptosporidium parvum Iowa II chromosome 8, whol... 28 5.9 gb|CM000434.1| Cryptosporidium parvum Iowa II chromosome 6, whol... 28 7.6 gb|CM000433.1| Cryptosporidium parvum Iowa II chromosome 5, whol... 28 10.0 >gb|CM000430.1| Cryptosporidium parvum Iowa II chromosome 2, whole genome shotgun sequence Length = 985969 Score = 394 bits (1013), Expect = e-110 Identities = 226/514 (43%), Positives = 313/514 (60%), Gaps = 17/514 (3%) Frame = -2 Query: 258 KLLQEDLAYDYDXXXXXXXXXXXXCAKEAAILGKKVMVLDFVVPSPQGTSWGLGGTCVNV 317 K++ DL + YD AKEAA GKKV + DFV PS QGT WGLGGTCVNV Sbjct: 966315 KIVMADLTFMYDLVVIGGGSGGMAAAKEAAKYGKKVALFDFVKPSTQGTKWGLGGTCVNV 966136 Query: 318 GCIPKKLMHQAALLGQALC-DSRKFGWEYNQQVRHNWETMTKAIQNHISSLNWGYRLSLR 376 GC+PKKLMH +AL+ ++ D++ FG + + W + + ++NHI LN+ YR LR Sbjct: 966135 GCVPKKLMHYSALIASSIHHDAQMFGHKTSSSFE--WGKLVETLRNHIRMLNFSYRTGLR 965962 Query: 377 EKAVAYVNSYGEFVEHHKIKATNKKGQETYYTAAQFVIATGERPRYLG-IQGDKEYCITS 435 V Y+N+ + ++ H ++ + GQ+ T+ ++ATG RP + G +Y ITS Sbjct: 965961 VGNVEYINALAKLIDPHSVEYEDN-GQKKTITSRYILLATGGRPSIPETVPGAIQYSITS 965785 Query: 436 DDLFSLPYCPGKTLVVGASYVALECAGFLAGFGLDVTVMVRSILLRGFDQEMAEKVGSYM 495 DD+F L PGKTLV+GASY+ LE AGFL G D TV +RSI LRGFD++ +EK+ YM Sbjct: 965784 DDIFFLSKSPGKTLVIGASYIGLETAGFLNELGFDTTVAMRSIPLRGFDRQCSEKIVEYM 965605 Query: 496 EQHGVKFLRKFIPVMVQQLEKGSPGKLKVLAKSTEGTETIEGVYNTVLLAIGRDSCTRKI 555 + G KFL +P+ ++++ + +KV E + TVL A GR+ + + Sbjct: 965604 KATGTKFLVGVVPINIEKVNEN----IKVSFSDGSVEE-----FETVLYATGRNPDVKGL 965452 Query: 556 GLEKIGVKINEKSGKIPVNDVEQTNVPYVYAVGDILEDKPELTPVAIQSGKLLAQRLFGA 615 L IGV++++ SGKI + + T+VP ++AVGDI+E +PELTPVA+++G LLA+RLF Sbjct: 965451 NLNAIGVEVSD-SGKI-IAPKDATSVPSIFAVGDIVEGRPELTPVAVKAGILLARRLFAG 965278 Query: 616 SLEKCDYINVPTTVFTPLEYGCCGLSEEKAIEVYKKENLEIYHTLFWPLEWTVAGRENN- 674 S E DY VPTTVFTP+EYG GLS E AI Y ++++E Y + F LE A RE Sbjct: 965277 SNEFIDYDFVPTTVFTPIEYGHVGLSSEAAIAKYGEDDIEEYLSEFSTLEIAAAHREKPE 965098 Query: 675 -------------TCYAKIICNKFDHDRVIGFHILGPNAGEVTQGFAAAMKCGLTKQLLD 721 C AK++ K ++V+GFH +GPNAGE+TQGF+ A+K G TK+ D Sbjct: 965097 HLRENEMDFALPLNCLAKLVVVKSQGEKVVGFHFVGPNAGEITQGFSLAVKLGATKKDFD 964918 Query: 722 DTIGIHPTCGEVFTTLEITKSSGLD-ITQKGCUG 754 D IGIHPT EVF LE+TK SG ++ GC G Sbjct: 964917 DMIGIHPTDAEVFGILEVTKRSGESFVSSGGCGG 964816 Score = 58.2 bits (139), Expect = 7e-09 Identities = 27/88 (30%), Positives = 43/88 (48%) Frame = +3 Query: 174 IERSRVVIFSKSYCPHSTRVKELFSSLGVECNVLELDQVDDGARVQEVLSEITNQKTVPN 233 I + + SKSYCP+ + S G V+++D D +Q+ E+T TVP Sbjct: 508605 ISSGDICVISKSYCPYCIKAINSLKSAGYSPLVMQIDGRVDTKEIQDYCRELTGSGTVPR 508784 Query: 234 IFVNKVHVGGCDQTFQAYQSGLLQKLLQ 261 +FV +GGCD T + + G L ++ Sbjct: 508785 VFVKGRFIGGCDDTLKLLEDGSLSSFVE 508868 Score = 27.7 bits (60), Expect = 10.0 Identities = 10/30 (33%), Positives = 18/30 (60%) Frame = -1 Query: 371 YRLSLREKAVAYVNSYGEFVEHHKIKATNK 400 YRL + E +++ YG+ + H +IK +K Sbjct: 224998 YRLGVLEIPECFLSKYGDMIRHSEIKTASK 224909 >gb|CM000436.1| Cryptosporidium parvum Iowa II chromosome 8, whole genome shotgun sequence Length = 1344712 Score = 28.5 bits (62), Expect = 5.9 Identities = 17/42 (40%), Positives = 18/42 (42%) Frame = -1 Query: 115 SPPQSPGPGKAGDAPNRRSGHVRGARVLSPPGRRARLSSPGP 156 SPP SP P P S V + LSP LSSP P Sbjct: 1309330 SPPPSPPPSPPTSPPKPPSPEVDSSSSLSPLFSPLPLSSPSP 1309205 Score = 27.7 bits (60), Expect = 10.0 Identities = 14/37 (37%), Positives = 17/37 (45%) Frame = +3 Query: 8 VSGKRRMHLTSRPVASTHSPLILDMCPHLNNGPMTGC 44 V + M SRP+ ST + MCPHL M C Sbjct: 39876 VCTQNSMSKRSRPLISTPPDMNQKMCPHLTESIMCEC 39986 >gb|CM000434.1| Cryptosporidium parvum Iowa II chromosome 6, whole genome shotgun sequence Length = 1332857 Score = 28.1 bits (61), Expect = 7.6 Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 4/46 (8%) Frame = +2 Query: 620 CDYINVPTTVFTPLEYGCCGLSEEKAIEVYKKEN----LEIYHTLF 661 C + +TVFT L+ GC K +E+Y K++ LE+ H + Sbjct: 536858 CKQVRFLSTVFTKLKIGC------KVLELYGKQSLQKRLEVVHNFY 536977 >gb|CM000433.1| Cryptosporidium parvum Iowa II chromosome 5, whole genome shotgun sequence Length = 1080900 Score = 27.7 bits (60), Expect = 10.0 Identities = 29/121 (23%), Positives = 51/121 (42%), Gaps = 12/121 (9%) Frame = +3 Query: 473 VMVRSILLRGF--------DQEMAEKVGSYMEQHGVKFLRKFIPVMVQQLEKGSPGKLKV 524 ++ RS+L+RGF D E EKV E +FLRK+I V K+K Sbjct: 1013967 ILQRSVLVRGFMQVWASGTDHEQVEKVLKEDEYVKKEFLRKYIHDDVD-----FSWKVKS 1014131 Query: 525 LAKSTEGTETIEGVYNTVLLAIGRDSCTRKIGLEKIGVKINEKS----GKIPVNDVEQTN 580 + K E + + + L G ++ K +G+ + +S K+P+ + + Sbjct: 1014132 IGKKLSRPEQVSRMNHYSFLFKGTETVNLKDPSLSLGIFEDWRSIHNQEKLPIEGISKEQ 1014311 Query: 581 V 581 + Sbjct: 1014312 I 1014314 Database: C.parvum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 9,102,324 Number of sequences in database: 8 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,185,584 Number of Sequences: 8 Number of extensions: 82260 Number of successful extensions: 335 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 276 Number of HSP's gapped (non-prelim): 96 length of query: 754 length of database: 3,034,108 effective HSP length: 99 effective length of query: 655 effective length of database: 3,033,316 effective search space: 1986821980 effective search space used: 1986821980 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 60 (27.7 bits)