TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000035_1.0 # Protein # Thioredoxin reductase 2 (TR2) # Homo sapiens # Complete (524 letters) Database: P.ultimum/genome.fa 82 sequences; 42,801,618 total letters Searching.................................................................................done Score E Sequences producing significant alignments: (bits) Value gb|GL376634.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 471 e-133 gb|GL376632.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 241 2e-63 gb|GL376566.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 189 6e-58 gb|GL376629.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 157 3e-38 gb|GL376573.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 60 7e-09 gb|GL376611.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 45 1e-04 gb|GL376636.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 40 0.008 >gb|GL376634.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582037, whole genome shotgun sequence Length = 1414051 Score = 471 bits (1213), Expect = e-133 Identities = 246/492 (50%), Positives = 335/492 (68%), Gaps = 6/492 (1%) Frame = -3 Query: 39 DYDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMH 98 DYD AC+KEAA+ G+KV V+DYV+PS GT WGLGGTCVNVGCIPKKLMH Sbjct: 1305434 DYDLVVIGGGSGGLACSKEAAKYGKKVCVLDYVKPSLVGTSWGLGGTCVNVGCIPKKLMH 1305255 Query: 99 QAALLGGLI-QDAPNYGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNI 157 Q++L+G I +D+ +GW V + DW +M ++V +++KS+N+ ++V+L+D+KVKY N+ Sbjct: 1305254 QSSLIGETIHKDSKAFGWTVGEQT-FDWAQMLKSVNSYIKSINFKYKVELRDKKVKYENL 1305078 Query: 158 KASFVDEHTV-CGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKE 216 SF+D HT+ K G + + + D ++IA GGRP+ P EG + I+SDDIFW KE Sbjct: 1305077 LGSFIDAHTLELWHPKKGTKTVTTRD-VVIAVGGRPK-PLACEGG-DLAISSDDIFWRKE 1304907 Query: 217 SPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGT-RF 275 +PGKTLVVGASYVALECAGF+ G+G D IM+RSI LRGFDQ M+ + E M F Sbjct: 1304906 APGKTLVVGASYVALECAGFMKGMGYDVKIMVRSILLRGFDQDMADKIGEFMEVESKIEF 1304727 Query: 276 LRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTS 335 +RGC P + +L +GQL V W + G+ FDTVL A GR PD L L+KAGV + Sbjct: 1304726 IRGCVPQSIMKLENGQLLVKWT-TADGEACEEAFDTVLNATGRDPDVAKLGLDKAGVKLN 1304550 Query: 336 PDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNV 395 P T +I V++ E TS P++YAIGDV++ PELTP+AI AGRLL +RL+ + LMDYD V Sbjct: 1304549 PKTGRIWVEN-EQTSTPNVYAIGDVIDA-PELTPVAIQAGRLLARRLYNNETTLMDYDKV 1304376 Query: 396 PTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRD---ASQCYVKMVC 452 T VFTP+EYGC GLSEE ++ + G++++E+YH ++ PLE++++ AS CYVK++C Sbjct: 1304375 CTAVFTPIEYGCCGLSEEASIEKFGEDNLEIYHQNFTPLEWSLSSATRPAASSCYVKVIC 1304196 Query: 453 LREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKR 512 + + V+G H+LGPNAGEVTQ L +K G +Y Q++ VGIHPT +E L ++K Sbjct: 1304195 DKSRDKYVVGFHYLGPNAGEVTQAMGLAMKLGFTYDQMVNVVGIHPTTAEIFTTLEVTKS 1304016 Query: 513 SGLDPTVTGCUG 524 SG GC G Sbjct: 1304015 SGASAAAGGC*G 1303980 >gb|GL376632.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582035, whole genome shotgun sequence Length = 696189 Score = 241 bits (614), Expect = 2e-63 Identities = 174/481 (36%), Positives = 252/481 (52%), Gaps = 13/481 (2%) Frame = +1 Query: 40 YDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRW---GLGGTCVNVGCIPKKL 96 YD A A+ AA G KV VV+ +G ++ GLGGTCVN GC+PKK+ Sbjct: 427336 YDYIAIGGGSGGMASARRAATYGAKVLVVE------RGRKYDGAGLGGTCVNYGCVPKKV 427497 Query: 97 MHQAAL-LGGLIQDAPNYGWEVAQPVPH---DWRKMAEAVQNHVKSLNWGHRVQLQDRKV 152 M AA + L ++A +P DW+ M ++ LN + L + ++ Sbjct: 427498 MFNAASHVEHLHRNADYSITTTTKPFEFGDFDWKNMKTKRDAYIARLNGIYERNLGNAQI 427677 Query: 153 KYFNIKASFV--DEHTVCGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDD 210 A V D+ V GV GK HI++A GG P P I G +E+ I SDD Sbjct: 427678 DLVEGVAKLVAKDKVEVDGVVYTGK-------HILVAVGGVPSLPP-IPG-IEHVIDSDD 427830 Query: 211 IFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMR-SIPLRGFDQQMSSMVIEHMA 269 F L+E P K VVGA Y+A+E AG + DT++ R LR FD + ++ E Sbjct: 427831 FFKLEEQPKKVAVVGAGYIAIEIAGIFNALKSDTSLFCRFDHVLRTFDPIVRDLINEEGE 428010 Query: 270 SHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDT-GTFDTVLWAIGRVPDTRSLNLE 328 G F+R R+ + DG LQ+T + G+E G F+ +L+AIGR P T+ L LE Sbjct: 428011 KAGINFVRNSRIERIDKQADGTLQITAK--VNGEEQVIGGFNQILYAIGREPRTKDLGLE 428184 Query: 329 KAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSD 388 + GV + + I+VD++E TSV IYAIGD ELTP+AI AGR L RLFGG + Sbjct: 428185 EVGVKLN-EKGFIVVDAQENTSVSGIYAIGDATTTGWELTPVAIAAGRRLSDRLFGGEPN 428361 Query: 389 -LMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRD-ASQC 446 + Y +PT VF+ G +G +E EA+ + G E+V+ Y + + L + +A D + Sbjct: 428362 ACLHYHQIPTVVFSHPPLGTIGYTEPEAIEKFGAENVKTYSSKFVNLLYAMADPDHKGKT 428541 Query: 447 YVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVK 506 +K+V + E ++V+G+H G A E+ QGF + +K A+ A V IHPT +EE+V Sbjct: 428542 AMKLVTVGE-DEVVVGIHVAGEGADEMIQGFGVAVKMNATKADFDNIVAIHPTAAEELVT 428718 Query: 507 L 507 + Sbjct: 428719 M 428721 >gb|GL376566.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581352, whole genome shotgun sequence Length = 165876 Score = 189 bits (481), Expect(2) = 6e-58 Identities = 144/397 (36%), Positives = 203/397 (51%), Gaps = 43/397 (10%) Frame = -1 Query: 154 YFNIKASFVD--EHTVCGVAKGGKEI---LLSADHIIIATGGRPRYPTHIEGALEYGITS 208 Y N+ S VD + T A+G E+ + A +I+IA GG+P P I G E+ I S Sbjct: 133893 YRNLANSKVDLLQGTAAFNAQGNVEVDGKEVKAKNILIAVGGKPLIPD-IPGK-EHCIDS 133720 Query: 209 DDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSI-PLRGFDQQMSSMVIEH 267 D F L+ P K VVGA Y+A+E AG L G+G DTTI R LR FD S ++ H Sbjct: 133719 DGFFELEVLPKKVAVVGAGYIAVELAGVLNGLGSDTTIFCRKHGVLRTFDVSRSVLLDVH 133540 Query: 268 -------------------------MASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTG 302 MA G +RV DG + +D G Sbjct: 133539 LTLMYPALNLSIFM*DSVRDMLHDAMAKDGITLRANSNVARVELAADGTKTLALQD---G 133369 Query: 303 KEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVE 362 E TG FDT+++A GRVP T SLNL AG+ T I VD+ +ATS +++A+GDV Sbjct: 133368 SEHTG-FDTIIYAAGRVPLTSSLNLSAAGIKTD-SHGFIKVDAYQATSKSNVFAVGDVC- 133198 Query: 363 GRPELTPIAIMAGRLLVQRLFGG---SSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARH 419 G LTP+AI AGR L RLFGG + + YDN+PT VF+ G +GL+E EA ++ Sbjct: 133197 GTTALTPVAIAAGRRLSDRLFGGERHADAKISYDNIPTVVFSHPPIGTIGLTEIEARKKY 133018 Query: 420 GQEHVEVYHAHYKPLEFTV---------AGRDASQCYVKMVCLREPPQLVLGLHFLGPNA 470 G ++ Y + + + + + A + +K+VCL + + V+GLH +G A Sbjct: 133017 GDAAIKTYTSRFVNMYYGLVNEVADDGKAAKPKPMTAMKLVCLGD-DEKVVGLHIIGMAA 132841 Query: 471 GEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKL 507 E+ QGF + +K GA+ A + IHPT +EE+V + Sbjct: 132840 DEMLQGFGVAVKMGATKADFDNCIAIHPTGAEELVTM 132730 Score = 54.7 bits (130), Expect(2) = 6e-58 Identities = 38/112 (33%), Positives = 56/112 (50%), Gaps = 8/112 (7%) Frame = -2 Query: 37 QRDYDXXXXXXXXXXXACAKEAAQL-GRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKK 95 Q +YD A ++ AA +VAVV+ LGGTCVNVGC+PKK Sbjct: 134297 QDEYDYLVIGAGSGGMASSRRAASYPNTRVAVVEKAR---------LGGTCVNVGCVPKK 134145 Query: 96 LMHQAALLGGLI-QDAPNYGWE------VAQPVPHDWRKMAEAVQNHVKSLN 140 +M+ AA + ++ +DA +YG+E + + V W K+ E +V LN Sbjct: 134144 IMYLAADMNHMLHRDAFHYGFETQDGKRIGENVSFHWNKLKERRDAYVLRLN 133989 >gb|GL376629.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582032, whole genome shotgun sequence Length = 863081 Score = 157 bits (396), Expect = 3e-38 Identities = 137/472 (29%), Positives = 208/472 (44%), Gaps = 8/472 (1%) Frame = +3 Query: 39 DYDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKK-LM 97 DYD A +AAQLG K A ++ +R LGGTC+NVGCIP K L+ Sbjct: 652239 DYDVVVIGGGPGGYVAAIKAAQLGLKTACIE--------SRGTLGGTCLNVGCIPSKALL 652394 Query: 98 HQAALLGGLIQDAPNYGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNI 157 H L D YG EV+ V D+ K+ ++ VK+L G + KV Y Sbjct: 652395 HSTHLYHSAQHDFSKYGIEVSG-VSMDFGKLMKSKDKAVKTLTGGIEQLFKKNKVTYIKG 652571 Query: 158 KASFVDEHTVCGVAKGGKEILLSADHIIIATGGR--PRYPTHIEGALEYGITSDDIFWLK 215 V ++ + +SA +I+IATG P P ++ A + S LK Sbjct: 652572 HGKIVAKNEIAVTLNDSGNQTVSAKNILIATGSEVTPLPPVPVDNAAGKIVDSTGALELK 652751 Query: 216 ESPGKTLVVGASYVALECAGFLTGIGLDTTIMM---RSIPLRGFDQQMSSMVIEHMASHG 272 + P +V+GA + LE +G T++ R P G D ++ + + G Sbjct: 652752 KVPEHLVVIGAGVIGLELGSVYKRLGAKVTVVEYLDRVCP--GLDLELGKEFTKLLKKQG 652925 Query: 273 TRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGV 332 F ++ ++ +T E + G T D VL A GR P T L LE G+ Sbjct: 652926 LGF-EFSTKVTASKVDGDKVTLTTEPAAGGAASTIECDAVLVATGRRPFTGGLGLENMGI 653102 Query: 333 DTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDY 392 T +I VD T +P+I+AIGDV+ G P L A G V+ + G ++Y Sbjct: 653103 QTDK-FGRIQVDEHFRTQIPNIFAIGDVIAG-PMLAHKAEEEGIACVETI-AGKHGHVNY 653273 Query: 393 DNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQC--YVKM 450 + +P ++T E VG +EE+ +E Y+ P+ R + VK+ Sbjct: 653274 NAIPGVIYTFPEVASVGKTEEQL-----KEEGVAYNVGKFPMLANSRARAVGEADGLVKV 653438 Query: 451 VCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSE 502 + + + +LG+H + NAGE+ LG++ GA+ + RT HPT SE Sbjct: 653439 IADKATDK-ILGVHIIASNAGEMISEGVLGMEYGAASEDIARTCHAHPTLSE 653591 >gb|GL376573.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581402, whole genome shotgun sequence Length = 979788 Score = 59.7 bits (143), Expect = 7e-09 Identities = 56/183 (30%), Positives = 90/183 (49%), Gaps = 4/183 (2%) Frame = +1 Query: 178 LLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFW--LKESPGKTLVVGASYVALECAG 235 +L+AD +IA G RP I + SD + W +K P + +VVGA + +E A Sbjct: 621916 VLTADKFLIAVGTRPARRPDIPFDGQAVFDSDQLLWGGVKTVPKRLIVVGAGVIGMEYAS 622095 Query: 236 FLTGI-GLDTTIMM-RSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQ 293 ++ I G++ TI+ R L D+++S + M G RF V ++ +G++ Sbjct: 622096 MMSIIPGMEVTIIDGRKEILDMADREVSEALCYSMRQTGARFFVEETIKSVEKV-NGEVI 622272 Query: 294 VTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPH 353 V +GK G D +L+ +GR +T LNL G++ + I VD T+VPH Sbjct: 622273 V---HLNSGKTLVG--DGLLYTLGRQGNTDGLNLPAVGLE-ADKRGLIQVDENFQTAVPH 622434 Query: 354 IYA 356 +YA Sbjct: 622435 MYA 622443 >gb|GL376611.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582014, whole genome shotgun sequence Length = 494327 Score = 45.4 bits (106), Expect = 1e-04 Identities = 57/251 (22%), Positives = 93/251 (37%), Gaps = 49/251 (19%) Frame = +3 Query: 39 DYDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMH 98 DYD CA E+A+ G++VA+VD + +GG CV+ G IP K Sbjct: 329151 DYDLVVIGSGPSATQCAIESAKQGKRVAIVD--------KKTSVGGVCVHTGTIPSKTFR 329306 Query: 99 QAAL--------------------------------LGGLIQDAPNYGWEVAQ---PVPH 123 +A L + G YG + + Sbjct: 329307 EAVLHLSGYRHHGYVLLPNRRY*FWCC*LR*LLFCYIRGAFAPYSFYGKSYSMKTVTIDD 329486 Query: 124 DWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTV-------CGVAKGGKE 176 ++ V + V + + QL+ +V+Y A F ++H + G ++ E Sbjct: 329487 ILYRVKRVVNSEVDVI----KAQLKAARVEYIPGFARFENDHEISIVRDPETGESQEAGE 329654 Query: 177 IL-----LSADHIIIATGGRPRYPTHIEGALEYGITSDDIFW--LKESPGKTLVVGASYV 229 I L AD +IA G RP + ++ + SD I +++ P +VVGA + Sbjct: 329655 ITNTVSRLRADKFLIACGTRPAHNPNVPIDGKVIFDSDQILSGDIRDLPRSLIVVGAGVI 329834 Query: 230 ALECAGFLTGI 240 +E A L I Sbjct: 329835 GIEYASMLNVI 329867 Score = 38.9 bits (89), Expect = 0.013 Identities = 43/166 (25%), Positives = 71/166 (42%), Gaps = 5/166 (3%) Frame = +2 Query: 355 YAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDL----MDYDNVPTTVFTPLEYGCVGL 410 YA+GD + G P L ++ GRL ++ L ++ N P ++T E VG Sbjct: 330518 YAVGDCI-GAPSLASTSMEQGRLAACHMWDQEEQLVTSQLENGNYPYGIYTIPEISMVGK 330694 Query: 411 SEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVL-GLHFLGPN 469 +E++ + + G + E+ A Y E S + L +P L L G+H +G Sbjct: 330695 TEQQ-LTKEGV-NFEIGIAKYS--ELAKGQMSGSTAGGMLKILFDPTTLKLFGVHAIGEG 330862 Query: 470 AGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGL 515 A E+ + I G S V +PT +E R++ +GL Sbjct: 330863 ATEIIHIGQVAIAMGCSVTYFRDAVFNYPTLAE---AYRVAALNGL 330991 >gb|GL376636.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875582039, whole genome shotgun sequence Length = 1829366 Score = 39.7 bits (91), Expect = 0.008 Identities = 54/209 (25%), Positives = 85/209 (40%), Gaps = 19/209 (9%) Frame = +1 Query: 175 KEILLSADHIIIATGGRPRYP----THIEGALEYGITSD--------DIFWLKESPGKT- 221 KE+++S DH++IATG P T + G Y D + +ES G + Sbjct: 713941 KELIVSYDHLVIATGASALVPNMQGTSLNGVFVYRTIEDLNSIIAYGEAQREQESQGVSK 714120 Query: 222 ----LVVGASYVALECAGFLTGIGLDTTIMMRSIPL--RGFDQQMSSMVIEHMASHGTRF 275 LV+G + LE A + + L I+ R L R D + ++ + S +F Sbjct: 714121 KKYALVIGGGLLGLEAAKAIHDLQLSVRIVNRGSRLMSRQLDDAGAKVLHNEIVS---KF 714291 Query: 276 LRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTS 335 + + G V D T G+ F +++A G P R +G+D + Sbjct: 714292 GIEVLYHKTTKYIHGTTVVEGVDFTDGESYDDVF-MIVFATGIKP--RDDLGRDSGLDVA 714462 Query: 336 PDTQKILVDSREATSVPHIYAIGDVVEGR 364 ILV+ TS +YAIG+ V R Sbjct: 714463 QRGGGILVNDLLETSHKDVYAIGECVSHR 714549 Database: P.ultimum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 42,801,618 Number of sequences in database: 82 Lambda K H 0.320 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 28,497,763 Number of Sequences: 82 Number of extensions: 450260 Number of successful extensions: 2089 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 1963 Number of HSP's gapped (non-prelim): 423 length of query: 524 length of database: 14,267,206 effective HSP length: 107 effective length of query: 417 effective length of database: 14,258,432 effective search space: 5945766144 effective search space used: 5945766144 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 64 (29.3 bits)