TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000035_1.0 # Protein # Thioredoxin reductase 2 (TR2) # Homo sapiens # Complete (524 letters) Database: P.pallidum/genome.fa 42 sequences; 32,942,533 total letters Searching..........................................done Score E Sequences producing significant alignments: (bits) Value gb|GL291012.1| Polysphondylium pallidum PN500 unplaced genomic s... 220 3e-60 gb|GL291013.1| Polysphondylium pallidum PN500 unplaced genomic s... 137 2e-32 gb|GL290994.1| Polysphondylium pallidum PN500 unplaced genomic s... 33 0.42 gb|GL290990.1| Polysphondylium pallidum PN500 unplaced genomic s... 32 1.2 gb|GL291005.1| Polysphondylium pallidum PN500 unplaced genomic s... 31 2.1 >gb|GL291012.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig15, whole genome shotgun sequence Length = 727357 Score = 220 bits (560), Expect(2) = 3e-60 Identities = 142/385 (36%), Positives = 212/385 (55%), Gaps = 2/385 (0%) Frame = -3 Query: 53 ACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAPN 112 A A+ AA+ G KV +++ +GGTCVNVGC+PKK+M AA + + AP Sbjct: 504038 ATARRAAKYGVKVGLIEKTR---------MGGTCVNVGCVPKKVMWNAATIKEALHAAPF 503886 Query: 113 YGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVAK 172 YG+E A V + W + + ++ LN + L VK N F + Sbjct: 503885 YGFEGADKVTYHWPTIKKNRDAYIARLNGIYNNMLAGSNVKSINGWGKFTGPRQ---LEV 503715 Query: 173 GGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALE 232 G+ + +AD+I+IATGG P+ P + GA E GITSD F L E P V+GA Y+A+E Sbjct: 503714 DGQ--VYTADNILIATGGYPQIP-QVPGA-ELGITSDGFFDLAELPKSVAVIGAGYIAVE 503547 Query: 233 CAGFLTGIGLDTTIMMRSIP-LRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQ 291 G L +G +++++R LR FD ++ + E M + G + S +R+ +GQ Sbjct: 503546 LVGILNTLGSKSSLVIRHDHFLRTFDDIVAHKLQEQMVTDGINIVTNSTISALRKTENGQ 503367 Query: 292 LQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSV 351 + V T + DTV+WAIGR P T+ + LE AGV + + I VD + T Sbjct: 503366 IVV-----VTNHTELPPVDTVIWAIGRDPSTKGIGLEAAGVSVN-ERGYIKVDEFQYTGA 503205 Query: 352 PHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDL-MDYDNVPTTVFTPLEYGCVGL 410 ++A+GDV G ELTP+AI AGR L +RLF L DY+N+P+ VF+ G VGL Sbjct: 503204 QGVFAVGDVT-GNKELTPVAIAAGRRLAERLFNKQEGLKFDYENIPSVVFSHPPIGTVGL 503028 Query: 411 SEEEAVARHGQEHVEVYHAHYKPLE 435 +E+EA+A++G+E+V+VY++ Y L+ Sbjct: 503027 TEKEAIAKYGKENVKVYNSRYVNLQ 502953 Score = 31.6 bits (70), Expect(2) = 3e-60 Identities = 15/58 (25%), Positives = 32/58 (55%) Frame = -1 Query: 427 YHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCG 484 Y+ + + + V + ++K++ + ++V G+H +G + E+ QGFA+ IK G Sbjct: 502888 YYCSFTNMYYAVVDDYKPKTFMKLIVTGKEEKVV-GIHSIGIGSDEMIQGFAVAIKMG 502718 >gb|GL291013.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig16, whole genome shotgun sequence Length = 228641 Score = 137 bits (346), Expect = 2e-32 Identities = 131/460 (28%), Positives = 208/460 (45%), Gaps = 6/460 (1%) Frame = -2 Query: 57 EAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAP----N 112 +A QLG KV VV+ R LGGTC+NVGCIP K + A+ +DA N Sbjct: 168982 KAGQLGMKVTVVE--------KRGKLGGTCLNVGCIPSKALLNAS---HKYEDAKTKFAN 168836 Query: 113 YGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVAK 172 YG +V V D M + + V L G + KV Y +TV + Sbjct: 168835 YGVKVGS-VELDLAAMMKYKEKSVNGLTSGIEGLFKKNKVTYAKGHGKITGPNTVEVTGE 168659 Query: 173 GGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALE 232 GK ++ +I+IATG ++ + ++S LK P + +V+G + LE Sbjct: 168658 DGKVQTITTKNIVIATGSEVASLPNVNIDEQTIVSSTGALALKAVPKRMVVIGGGVIGLE 168479 Query: 233 CAGFLTGIGLDTTIMMRSIPLR-GFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQ 291 + +G +TT++ + + G D +++ + + +F + V + +G Sbjct: 168478 LGSVWSRLGAETTVVEFTNRIAAGADGEVAKKFQKVLEKQHMKFHLETKVTSVVKNANGT 168299 Query: 292 LQVTWED-STTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATS 350 + VT E G D VL ++GR P+T +L L+K GV ++ V T+ Sbjct: 168298 VTVTVESVGAGGFSGQIEADVVLVSVGRRPNTTNLGLDKVGVPMDK-AGRVEVGDHFKTN 168122 Query: 351 VPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGL 410 V IYAIGD ++G P L A G +++ L GS + +Y +P+ ++T E VG Sbjct: 168121 VKSIYAIGDAIKG-PMLAHKAEEEGIAVMEYLHNGSGHV-NYAAIPSVIYTHPEVAWVGK 167948 Query: 411 SEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNA 470 +EEE + +V + T D S+ +VK + + +LG+H +G A Sbjct: 167947 TEEELTKEGIKFNVGKFPYAANSRART---NDESEGFVKFLSDSSTDR-ILGVHIMGDCA 167780 Query: 471 GEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRIS 510 GE+ L ++ GAS V RT HPT SE V + +S Sbjct: 167779 GEMIAESVLAMEYGASSEDVARTCHAHPTLSEAVKEAAMS 167660 >gb|GL290994.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold12, whole genome shotgun sequence Length = 1032189 Score = 33.5 bits (75), Expect = 0.42 Identities = 45/200 (22%), Positives = 77/200 (38%), Gaps = 18/200 (9%) Frame = +1 Query: 179 LSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTL--------------VV 224 + + II+ATG A + G+ ++ +W K + VV Sbjct: 986686 IQSKSIILATGAT---------AKKMGVPGEETYWSKGISACAVCDGALPIYRNKHLAVV 986838 Query: 225 GASYVALECAGFLTGIGLDTTIMMRSIPLRGF----DQQMSSMVIEHMASHGTRFLRGCA 280 G A E A FLT T+++R +R + +++ IE + +H +G Sbjct: 986839 GGGDTACEEATFLTHFASKVTMLVRRDEMRASKAMQQRVLTNPKIEIIWNHTLLSAQG-- 987012 Query: 281 PSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQK 340 D +T ++ T +E + +AIG VP+T LN ++T Sbjct: 987013 -------GDKLTDLTIQNVLTKEEKKLEVGGLFYAIGHVPNTAFLN---GQIETDETGYI 987162 Query: 341 ILVDSREATSVPHIYAIGDV 360 I T+V ++A GDV Sbjct: 987163 ITKGHSTETNVKGVFACGDV 987222 >gb|GL290990.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold8, whole genome shotgun sequence Length = 2270872 Score = 32.0 bits (71), Expect = 1.2 Identities = 13/28 (46%), Positives = 17/28 (60%) Frame = -1 Query: 273 TRFLRGCAPSRVRRLPDGQLQVTWEDST 300 +RF +GC P + R PDGQ + W ST Sbjct: 964240 SRFPKGCQPRK*SRFPDGQKHLRWTIST 964157 >gb|GL291005.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig8, whole genome shotgun sequence Length = 1319729 Score = 31.2 bits (69), Expect = 2.1 Identities = 34/156 (21%), Positives = 60/156 (38%), Gaps = 13/156 (8%) Frame = +3 Query: 207 TSDDIFWLKESPGK--TLVVGASYVALECAGFLTGIGLDTTIMMRS----IPLRGFDQQM 260 T + ++WLK GK T + S +C L ++ + + FD+ + Sbjct: 747012 TDNCVYWLKTQDGKNWTYPIKISNETTDCGPTLCNNSNTLVCFIKGQGGILKMSTFDRSL 747191 Query: 261 SSMVIEHMASHGTRFLRGCAPSRVRR------LPDGQLQVTWEDSTTGKEDT-GTFDTVL 313 +S ++ + G P + LP+G++ V W+D + T TFD Sbjct: 747192 NSWSEFTTPTYYWAYKGGYVPINLDTAPTSVYLPNGKVMVLWQDPSDDNYITYSTFDGKS 747371 Query: 314 WAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREAT 349 WA+ V T + V S ++I V + T Sbjct: 747372 WALPVVVGTGTYEKTPYPVGVSRWNKQIAVGYNDTT 747479 Database: P.pallidum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 32,942,533 Number of sequences in database: 42 Lambda K H 0.320 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,558,832 Number of Sequences: 42 Number of extensions: 201719 Number of successful extensions: 672 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 639 Number of HSP's gapped (non-prelim): 83 length of query: 524 length of database: 10,980,844 effective HSP length: 105 effective length of query: 419 effective length of database: 10,976,434 effective search space: 4599125846 effective search space used: 4599125846 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 63 (28.9 bits)