TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000035_1.0 # Protein # Thioredoxin reductase 2 (TR2) # Homo sapiens # Complete (524 letters) Database: N.gruberi/genome.fa 784 sequences; 40,964,085 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG738881.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 415 e-116 gb|GG738854.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 248 e-105 gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 366 e-101 gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 130 3e-30 gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 54 4e-07 gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 45 2e-04 >gb|GG738881.1| Naegleria gruberi genomic scaffold NAEGRscaffold_37, whole genome shotgun sequence Length = 379849 Score = 415 bits (1066), Expect = e-116 Identities = 223/443 (50%), Positives = 291/443 (65%), Gaps = 7/443 (1%) Frame = -3 Query: 89 VGCIPKKLMHQAALLGGLIQDAPNYGWE------VAQPVPHDWRKMAEAVQNHVKSLNWG 142 VGCIPKKLMH AAL+G QD +GW + H W K+ E VQ+H+ LN+ Sbjct: 49346 VGCIPKKLMHTAALIGDRSQDGTAFGWTNSVGHGTERKPHHQWGKLVEMVQDHIHGLNFK 49167 Query: 143 HRVQLQDRKVKYFNIKASFVDEHTVCGVAKGGKE-ILLSADHIIIATGGRPRYPTHIEGA 201 +R L+ + + Y N+ ASF+D HTV G G +E +A IA GGRP YP I GA Sbjct: 49166 YRTDLRKKGITYHNLYASFIDAHTVEGKKDGQEEGKRFTARRFAIAVGGRPLYPD-IPGA 48990 Query: 202 LEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMS 261 EY I+SDDIF +++ PGK+LVVGASYVALEC GFL GIG DTTIM+RS+PLRGFD++ + Sbjct: 48989 KEYCISSDDIFSMEKEPGKSLVVGASYVALECGGFLHGIGNDTTIMVRSVPLRGFDRECA 48810 Query: 262 SMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPD 321 ++ M+ G +F + C P V +L G+L V +E+ T ++ T FDTVL+A GR Sbjct: 48809 DKIVNVMSDEGVKFYQPCVPLSVNKLESGKLSVEYENVETKEKKTEEFDTVLFATGRYAL 48630 Query: 322 TRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQR 381 T++LNLE GV T KI V+ E +SVPHIYAIGDV+ G ELTP+AI AG+LL R Sbjct: 48629 TQALNLENIGVKTD-KVGKIFVNKYEQSSVPHIYAIGDVIVGGLELTPVAIKAGKLLAAR 48453 Query: 382 LFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGR 441 L+ S +MDYD VPTTVFTP+EYG GL+EEEAV R+G E++ +Y Y LE+ R Sbjct: 48452 LYNSSKKMMDYDKVPTTVFTPVEYGSCGLTEEEAVKRYGAENLTIYKKSYYVLEYEPPAR 48273 Query: 442 DASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCS 501 + Q +VK++C + VLG H++GPNAGEVTQGFA+ ++C A+ TVGIHPTC+ Sbjct: 48272 EC-QAFVKLICNELDNERVLGFHYVGPNAGEVTQGFAVAMRCNATKEDFDDTVGIHPTCA 48096 Query: 502 EEVVKLRISKRSGLDPTVTGCUG 524 E +VKL G++ T TGC G Sbjct: 48095 ETIVKL----EKGVE-TDTGC*G 48042 Score = 65.5 bits (158), Expect = 1e-10 Identities = 31/50 (62%), Positives = 33/50 (66%) Frame = -3 Query: 39 DYDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVN 88 DYD A AKEA LG KVAV DY++PSPQGT WGLGGTCVN Sbjct: 49706 DYDLIVIGGGSGGIALAKEANALGAKVAVFDYIKPSPQGTTWGLGGTCVN 49557 >gb|GG738854.1| Naegleria gruberi genomic scaffold NAEGRscaffold_10, whole genome shotgun sequence Length = 665159 Score = 248 bits (634), Expect(3) = e-105 Identities = 137/330 (41%), Positives = 196/330 (59%), Gaps = 32/330 (9%) Frame = -2 Query: 64 KVAVVDYVEPSPQ----------GTRWGLGGTCVNVGCIPKKLMHQAALLG-GLIQDAPN 112 ++ ++DY +PS Q G W LGGTCVNVGCIPKKLMH +AL+G G+ DA Sbjct: 89371 RICLLDYRDPSSQWLRERNGKLNGWMWQLGGTCVNVGCIPKKLMHNSALIGEGINHDAEY 89192 Query: 113 YGWEVAQPV-----PHDWRKMAEAVQNHVKSLNWGHRVQLQD-----------RKVKYFN 156 +GW + HDW+ + VQ ++KSLN+G+R + +K+ Y N Sbjct: 89191 FGWNDGKQEGKLSGKHDWKTLVANVQQYIKSLNFGYRTSVTQTYAFDNEDPSLKKLYYLN 89012 Query: 157 IKASFVDEHTV-CGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLK 215 +K FVD+HT+ + G +S II++ GG P P + GA EY ITSDDIF LK Sbjct: 89011 MKGRFVDDHTLELTDPRKGITKTISGKVIILSVGGTPNIPQDVPGATEYAITSDDIFSLK 88832 Query: 216 ESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRF 275 E PGKTLV+GASY+ALE A FL G+G ++++MMRSIPLRGFDQ+ + V++ M G +F Sbjct: 88831 EEPGKTLVIGASYIALETASFLKGLGYESSVMMRSIPLRGFDQECAWKVVDGMKERGVKF 88652 Query: 276 LRGCAPSRVRRLPD----GQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAG 331 L C P ++ + D G+ V ++D +T + T + T+++A+GR T+ LN++ Sbjct: 88651 LEQCIPVKIEKSSDHETTGEYTVHYKDLSTQQVYTENYKTIMFAVGRRAVTKELNIDL-- 88478 Query: 332 VDTSPDTQKILVDSREATSVPHIYAIGDVV 361 ++ KI+V E TS HIYAIGD + Sbjct: 88477 --DMDESGKIIVSDNEQTSKKHIYAIGDCI 88394 Score = 135 bits (340), Expect(3) = e-105 Identities = 62/120 (51%), Positives = 90/120 (75%), Gaps = 1/120 (0%) Frame = -1 Query: 364 RPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFT-PLEYGCVGLSEEEAVARHGQE 422 + ELTP+AI AG+LL RL ++LMDYDNVPT++FT P EYGC GLSEEEAV R+G++ Sbjct: 88343 KTELTPVAIRAGKLLASRLTNKGTELMDYDNVPTSIFTHPYEYGCCGLSEEEAVQRYGED 88164 Query: 423 HVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIK 482 +EVY + Y P+E ++ RD ++ ++K++ L+ + V+G H++G NAGE+TQG A+ IK Sbjct: 88163 DIEVYISLYSPIEHQLSHRDTNKTFMKIITLKSANEKVIGFHYVGANAGEITQGIAVAIK 87984 Score = 39.7 bits (91), Expect(3) = e-105 Identities = 19/43 (44%), Positives = 25/43 (58%) Frame = -3 Query: 482 KCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCUG 524 + GA+ T+GIHPT +EE+ L I+KRSG GC G Sbjct: 87933 RAGATKEHFDNTIGIHPTAAEEMTLLSITKRSGNSAEKDGC*G 87805 >gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_8, whole genome shotgun sequence Length = 938756 Score = 366 bits (940), Expect = e-101 Identities = 216/525 (41%), Positives = 304/525 (57%), Gaps = 55/525 (10%) Frame = +1 Query: 40 YDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVN----------- 88 YD C+K AA LG KVA++DYV+PS QG+ W +GGTCVN Sbjct: 731830 YDLIVVGGGSGGLKCSKLAADLGAKVALLDYVKPSSQGSTWKIGGTCVNGM*WID*LRWS 732009 Query: 89 ------------------VGCIPKKLMHQAALLGGLIQDAPNYGWEVAQPV--------- 121 VGCIPKK MH A+ +G I++A NYGW++ + Sbjct: 732010 TNDFNILYMHVCS*LK*SVGCIPKKQMHFASEMGNKIREAINYGWKLPLGLGEAPEEDMF 732189 Query: 122 ---PH-------DWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVA 171 PH DW + + V N N+ ++ +L+ RK+ ++ VD HTV V Sbjct: 732190 NNFPHNKRGEFFDWTTLVQGVLNTRSGSNFVYKGELRKRKIDFYEALGRLVDSHTVELV- 732366 Query: 172 KGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVAL 231 G+ L+A +I++A GGRP P I GA EY ITSDD+F L+++PGKTLVVG SY+AL Sbjct: 732367 --GRVERLTAKYILLAPGGRPNVPRDIPGAYEYAITSDDLFSLQQAPGKTLVVGGSYIAL 732540 Query: 232 ECAGFLTGIGLDTTIMMRSIPLR--GFDQQMSSMVIEHM-ASHGTRFLRGCAPSRVRRLP 288 ECAGFL+G+G D ++M+RS LR FD +++S++ E M H +FL C P ++ +L Sbjct: 732541 ECAGFLSGLGFDVSVMVRSRILRSEAFDAEIASILRESMELHHHVKFLEPCIPVKIEKLE 732720 Query: 289 DGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREA 348 + Q +VT+ ++T KE T FDTV++A GR D + LNL GV + + KI+V+ E Sbjct: 732721 NNQKRVTFLNATENKEYTQDFDTVMFATGRYADVQGLNLAAIGVQHTKE-GKIIVNDEER 732897 Query: 349 TSVPHIYAIGDVVEG--RPELTPIAIMAGRLLVQRLFGGS--SDLMDYDNVPTTVFTPLE 404 TSV IYAIGDV+E ELTP+AI G+ L RLF + +DYD VPTTVFTP E Sbjct: 732898 TSVESIYAIGDVIENGHNYELTPVAIQQGKYLAYRLFKPEEINKKVDYDFVPTTVFTPTE 733077 Query: 405 YGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLH 464 YG VG SEE+A G+ ++ +Y + LE +A + +VK++C++ + V+GLH Sbjct: 733078 YGLVGYSEEKAKKVFGENNLVIYKKKFNILEHKIA-EIGEKGFVKLICVKNQNERVVGLH 733254 Query: 465 FLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRI 509 +LGPNA EVTQGFAL +K G + + +GIHP+ +E + L + Sbjct: 733255 YLGPNAAEVTQGFALALKKGCTKEEFDDVIGIHPSNAEAFMYLEL 733389 >gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_9, whole genome shotgun sequence Length = 695587 Score = 130 bits (327), Expect = 3e-30 Identities = 106/380 (27%), Positives = 179/380 (47%), Gaps = 1/380 (0%) Frame = +3 Query: 136 VKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVAKGGKEILLSADHIIIATGGRPRYP 195 VK L G + KV+Y + + ++ V GK ++ D+I+IATG Sbjct: 643653 VKGLTGGIEFLFKKYKVEYVKGEGTVTGKNEVSVKGLDGKNSTIATDNIVIATGSEATPL 643832 Query: 196 THIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRG 255 + + ++S L P + V+GA + LE + +G + T++ S + Sbjct: 643833 PFLPFDEKVILSSTGALALDHVPKRMTVIGAGVIGLEMGSVYSRLGSEVTVIEYSDRISP 644012 Query: 256 F-DQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLW 314 F D+++S++ + M G +F G + + + Q+ ++ TG +T D L Sbjct: 644013 FLDKEVSAVFQKTMEKLGMKFKLGVSVKSGKVVDGKTCQLELVNNATGAAETFESDVCLV 644192 Query: 315 AIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMA 374 +IGR P T +L L G+ T + ++ VD TSV +IYAIGDVV G P L A Sbjct: 644193 SIGRRPHTANLGLNNVGI-TLDERGRVPVDDHFRTSVSNIYAIGDVVRG-PMLAHKAEDE 644366 Query: 375 GRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPL 434 G + ++L G S + +YD +P ++T E VG +EEE V + V + Sbjct: 644367 GIAIAEQLAGRSGHV-NYDAIPNVIYTHPEIASVGKTEEELVKAGIKYKVGKFPYSANSR 644543 Query: 435 EFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTV 494 T+ D ++ +VK++ + + VLG+H +G AGE+ L ++ GAS + RT Sbjct: 644544 ARTID--DGTEGFVKILADAQTDK-VLGVHIIGIYAGELIAEATLAMEYGASSEDIARTC 644714 Query: 495 GIHPTCSEEVVKLRISKRSG 514 HPT SE + + ++ G Sbjct: 644715 HAHPTLSEAIKEAAVAAYDG 644774 Score = 32.7 bits (73), Expect = 0.88 Identities = 18/37 (48%), Positives = 22/37 (59%) Frame = +2 Query: 55 AKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGC 91 A +A+QLG K A V+ R LGGTC+NVGC Sbjct: 643274 AIKASQLGLKTACVE--------KRGSLGGTCLNVGC 643360 >gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_62, whole genome shotgun sequence Length = 286411 Score = 53.9 bits (128), Expect = 4e-07 Identities = 42/144 (29%), Positives = 70/144 (48%), Gaps = 5/144 (3%) Frame = +3 Query: 222 LVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASH-GTRFLRGCA 280 +V+GA Y+A E A F G T I +RS L+ D+++ + ++G Sbjct: 181326 IVIGAGYIATELAHFFASTGTKTNIFVRSGFLKHEDEEVQKEFDRVFSKKKNINVIKGMK 181505 Query: 281 PSRVRRLPDGQLQVTWEDSTTGKEDTGTFDT----VLWAIGRVPDTRSLNLEKAGVDTSP 336 + R D + +T+ S E +GT + +L A G VP+T L +KAG+ TS Sbjct: 181506 ILKARYEND-EFVLTY--SNPSDESSGTIEVRAEGLLVAAGVVPNTDILQCDKAGIQTSK 181676 Query: 337 DTQKILVDSREATSVPHIYAIGDV 360 D I+ ++ + P+++A GDV Sbjct: 181677 DGFIIVDKFLKSVNAPNVWAFGDV 181748 >gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_3, whole genome shotgun sequence Length = 941567 Score = 44.7 bits (104), Expect = 2e-04 Identities = 22/31 (70%), Positives = 24/31 (77%), Gaps = 1/31 (3%) Frame = -2 Query: 389 LMDYDNVPTTVFTP-LEYGCVGLSEEEAVAR 418 LMDYDNV T++F EYGC GLSEEEAV R Sbjct: 100426 LMDYDNVTTSIFNH*YEYGCCGLSEEEAVQR 100334 Database: N.gruberi/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 40,964,085 Number of sequences in database: 784 Lambda K H 0.320 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,583,806 Number of Sequences: 784 Number of extensions: 270968 Number of successful extensions: 956 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 839 Number of HSP's gapped (non-prelim): 227 length of query: 524 length of database: 13,654,695 effective HSP length: 107 effective length of query: 417 effective length of database: 13,570,807 effective search space: 5659026519 effective search space used: 5659026519 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 64 (29.3 bits)