TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000035_1.0 # Protein # Thioredoxin reductase 2 (TR2) # Homo sapiens # Complete (524 letters) Database: H.arabidopsidis/genome.fa 5422 sequences; 67,459,135 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ABWE01000075.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 456 e-128 gb|ABWE01000835.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 242 1e-63 gb|ABWE01001242.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 142 2e-40 gb|ABWE01000290.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 77 5e-14 gb|ABWE01003496.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 71 5e-12 gb|ABWE01000605.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 54 5e-07 gb|ABWE01000612.1| Hyaloperonospora parasitica strain Emoy2 v-7.... 49 2e-05 >gb|ABWE01000075.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont51.5, whole genome shotgun sequence Length = 90922 Score = 456 bits (1172), Expect = e-128 Identities = 248/500 (49%), Positives = 330/500 (66%), Gaps = 14/500 (2%) Frame = +3 Query: 39 DYDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMH 98 DYD AC+KEAA G+KV V+DYV+PSP GT WGLGGTCVNVGCIPKKLMH Sbjct: 26040 DYDLVVIGGGSGGLACSKEAASFGKKVCVLDYVKPSPHGTSWGLGGTCVNVGCIPKKLMH 26219 Query: 99 QAALLGGLI-QDAPNYGWEV----AQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVK 153 Q++L+G ++ D ++GW V Q DW+++ V +++KS+++ ++V+L+ ++VK Sbjct: 26220 QSSLIGEILHHDCASFGWNVGSVNGQVPTFDWKQLVSNVSSYIKSISFKYKVELRSKQVK 26399 Query: 154 YFNIKASFVDEHT--VCGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDI 211 Y N+ SFVD HT + KG KEI + H++IA GGRPR + G E ++SDDI Sbjct: 26400 YENVLGSFVDPHTLKLWHPKKGTKEI--TTRHVVIAVGGRPRQLACLGG--ELAVSSDDI 26567 Query: 212 FWLKE-SPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHM-A 269 FW+K+ PGKTLVVGASYVALECAGFL G+G +M+RSI LRGFDQ M+S + E+M Sbjct: 26568 FWMKQKEPGKTLVVGASYVALECAGFLKGMGYAVKVMVRSILLRGFDQDMASKIGEYMEM 26747 Query: 270 SHGTRFLRGCAPSRVRRLPDGQLQVTW--EDSTTGKEDTGTFDTVLWAIGRVPDTRSLNL 327 G F+R P + +L GQL V W ED +E TFDTVL A GR PD L L Sbjct: 26748 QSGIEFIRKTVPQSITKLDSGQLLVKWITEDGDCFEE---TFDTVLNATGRDPDVAKLGL 26918 Query: 328 EKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSS 387 ++AGV + T +I V++ E T+ P++YAIGDV++ PELTP+AI AGRLL +RL+ + Sbjct: 26919 DEAGVKLNEKTGRIWVNN-EQTTTPNVYAIGDVIDA-PELTPVAIQAGRLLSRRLYNEGT 27092 Query: 388 DLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRD---AS 444 MDYD + T VFTP+EYGC GLSEE++ +G + VEVYH ++ PLE+++A + A Sbjct: 27093 VQMDYDKICTAVFTPIEYGCCGLSEEDSKEEYGDDSVEVYHKNFVPLEWSLAEQTRIAAE 27272 Query: 445 QCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEV 504 CYVK+VC + VLG H+LGPNAGEVTQ L +K G +Y Q++ TVGIHPT +E Sbjct: 27273 SCYVKVVCDKTRNNFVLGFHYLGPNAGEVTQAMGLAMKLGFTYDQLVDTVGIHPTTAEAF 27452 Query: 505 VKLRISKRSGLDPTVTGCUG 524 L +K SG T GC G Sbjct: 27453 TTLETTKSSGDSTTGGGC*G 27512 >gb|ABWE01000835.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont130.5, whole genome shotgun sequence Length = 26476 Score = 242 bits (617), Expect = 1e-63 Identities = 168/473 (35%), Positives = 248/473 (52%), Gaps = 5/473 (1%) Frame = -2 Query: 40 YDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQ 99 YD A A+ AA G KV V+ E Q GLGGTCVN GC+PKK+M Sbjct: 7131 YDYIVIGGGSGGMASARRAATYGAKVLVI---ERGRQYDGMGLGGTCVNFGCVPKKIMFN 6961 Query: 100 AALLGGLIQDAPNYGWEVAQPV--PHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNI 157 A + A +Y A+ DW KM ++ L ++ L + +V + Sbjct: 6960 TAAHVEHLNRAKDYSIHTAEFAFGDFDWSKMKTKRDAYINRLTGIYQRNLGNARVDHVEG 6781 Query: 158 KASFVDEHTVCGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKES 217 A FV ++ V + + + H++IA GG P P + G + + I SDD F L++ Sbjct: 6780 IAKFVAKNKVQVADR-----VFTGKHVLIAPGGVPLKPD-LPG-INHVIDSDDFFRLEQQ 6622 Query: 218 PGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIP-LRGFDQQMSSMVIEHMASHGTRFL 276 P K VVGA Y+A+E AG + DTT+ R LR F+ + +V E M G F+ Sbjct: 6621 PQKVAVVGAGYIAVEMAGIFHALKSDTTLFCRHDQVLRRFEPIVRDLVNEEMEKAGVTFV 6442 Query: 277 RGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSP 336 R + V + DG+L + ++ T FDT+L+A+GR P T L LE GV + Sbjct: 6441 RNMSAVGVEKQLDGKLTLVVNVHNEEQKFTD-FDTILFAVGRKPRTEDLGLEDIGVKLA- 6268 Query: 337 DTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSD-LMDYDNV 395 + I VD++E TSVP +YAIGD ELTP+AI AGR L RLFGG D ++Y + Sbjct: 6267 EGGFIQVDAQENTSVPGVYAIGDATVTGWELTPVAIAAGRRLADRLFGGEKDACLNYHQI 6088 Query: 396 PTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRD-ASQCYVKMVCLR 454 P+ +F+ G +G +E EAVA++G+E+V+VY + + L ++A + + +K+V + Sbjct: 6087 PSVIFSHPPIGTIGYTEPEAVAKYGEENVKVYTSKFVNLLHSMADPEHKGKTAMKLVTVG 5908 Query: 455 EPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKL 507 E + V+G+H G A E+ QGF + +K A+ A V IHPT SEE+V + Sbjct: 5907 E-QETVVGVHVAGEGADEMIQGFGVAVKMNATKADFDNIVAIHPTASEELVTM 5752 >gb|ABWE01001242.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont26.2, whole genome shotgun sequence Length = 16867 Score = 142 bits (359), Expect(2) = 2e-40 Identities = 95/267 (35%), Positives = 143/267 (53%), Gaps = 10/267 (3%) Frame = -2 Query: 268 MASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNL 327 MA G +RV + DG + D G G FD V++A GRVP T L+L Sbjct: 6570 MAIDGLHLRPNSNVARVEQAADGTNTLVLTD---GSAHCG-FDIVIYAAGRVPLTDKLDL 6403 Query: 328 EKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSS 387 + AGV T+ I+V+ + TS ++A+GDV G P LTP+A+ AGR L RLFG Sbjct: 6402 DNAGVATNKHGY-IVVNEFQETSNSKVFAVGDVC-GTPALTPVAVAAGRKLSDRLFGNMK 6229 Query: 388 DL-MDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPL---------EFT 437 D + Y+N+PT VF+ G +GL+E++A ++G + ++VY +H+ + E T Sbjct: 6228 DARVSYENIPTVVFSHPPIGTIGLTEKQAQGKYGDDDIKVYTSHFVNMYYGLINEVDEAT 6049 Query: 438 VAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIH 497 ++ + +K+VC + ++V GLH +G A E+ QGF + +K GA+ A + IH Sbjct: 6048 GELKEKPKTAIKLVCAGKKEKVV-GLHMIGMAADEILQGFGVAVKMGATKADFDNCIAIH 5872 Query: 498 PTCSEEVVKLRISKRSGLDPTVTGCUG 524 PT SEE+V L SG + C G Sbjct: 5871 PTASEELVTLAPWGLSGYE*AKWICNG 5791 Score = 53.9 bits (128), Expect = 6e-07 Identities = 38/112 (33%), Positives = 54/112 (48%), Gaps = 9/112 (8%) Frame = -3 Query: 38 RDYDXXXXXXXXXXXACAKEAAQL-GRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKL 96 ++YD A A+ AA +VAVV++ LGGTCVNVGC+PKKL Sbjct: 7517 KEYDYLVIGGGSGGIASARRAASYPNTRVAVVEHAR---------LGGTCVNVGCVPKKL 7365 Query: 97 MHQAALLGGLIQ-DAPNYGWE-------VAQPVPHDWRKMAEAVQNHVKSLN 140 M AA + +Q D +YG+E + + DW K+ +V+ LN Sbjct: 7364 MFLAADMSHKLQHDVVHYGFEKEGTGGSLGAQMHFDWTKLKTRRDAYVRRLN 7209 Score = 43.5 bits (101), Expect(2) = 2e-40 Identities = 26/62 (41%), Positives = 34/62 (54%), Gaps = 1/62 (1%) Frame = -3 Query: 203 EYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSI-PLRGFDQQMS 261 E I SD F L P K V+GA Y+A+E AG L +G +T+I R LR FD + Sbjct: 6836 ELCIDSDGFFELDTLPKKVAVIGAGYIAVELAGVLNALGSETSIFCRKEGVLRTFDVRSR 6657 Query: 262 SM 263 S+ Sbjct: 6656 SL 6651 >gb|ABWE01000290.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont22.4, whole genome shotgun sequence Length = 52974 Score = 77.4 bits (189), Expect = 5e-14 Identities = 62/184 (33%), Positives = 96/184 (52%), Gaps = 5/184 (2%) Frame = +1 Query: 178 LLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFW--LKESPGKTLVVGASYVALECAG 235 +L+AD+ ++ G RP I E SD + W +K P + +VVGA V +E A Sbjct: 51463 VLTADNFLVCVGTRPARRADIPFDGETIFDSDQLLWGKVKAVPRRLIVVGAGVVGMEYAS 51642 Query: 236 FLTGI-GLDTTIMM-RSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQ 293 ++ I G D T++ R L D+++S + MA GTRFL V + G++ Sbjct: 51643 MMSIIPGTDVTVIDGRKEILEMADKEVSEALCYSMAQTGTRFLVDEVIKSVEKSAKGEVG 51822 Query: 294 VTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQ-KILVDSREATSVP 352 V E +GK G D +L+ +GR + LNLE G+ +PD + +I+VD+ T+VP Sbjct: 51823 VHLE---SGKTVVG--DGLLYTVGRQGNVEGLNLEAVGL--APDKRGRIIVDNNFQTAVP 51981 Query: 353 HIYA 356 H+YA Sbjct: 51982 HMYA 51993 Score = 31.6 bits (70), Expect = 3.2 Identities = 17/46 (36%), Positives = 26/46 (56%) Frame = +1 Query: 54 CAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQ 99 CA ++A+ G VA+VD R LGG CV+ G +P K+ ++ Sbjct: 50749 CAIDSAKHGYSVAIVD--------KRDMLGGVCVHTGTVPSKVPYR 50862 >gb|ABWE01003496.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont52.4, whole genome shotgun sequence Length = 1688 Score = 70.9 bits (172), Expect = 5e-12 Identities = 75/272 (27%), Positives = 110/272 (40%), Gaps = 4/272 (1%) Frame = -1 Query: 39 DYDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKK-LM 97 DYD A +AAQLG K A V+ +R LGGTC+NVGCIP K L+ Sbjct: 773 DYDLVVIGGGPGGYVAAIKAAQLGLKTACVE--------SRGKLGGTCLNVGCIPSKALL 618 Query: 98 HQAALLGGLIQDAPNYGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNI 157 H LL + +YG + A V ++ +M +A + V++L G KV Y Sbjct: 617 HSTHLLHTAQHEFKSYGID-APNVTANFPQMMKAKEKAVRTLTAGIESLFSKNKVTYIKG 441 Query: 158 KASFVDEHTV-CGVAKGGKEILLSADHIIIATGGR--PRYPTHIEGALEYGITSDDIFWL 214 + V +A L+A +II+ATG P P ++ + S L Sbjct: 440 VGKLAAKGQVSVTLADRQGNGTLTAKNIILATGSEVTPLPPVPVDNDAGKIVDSTGALAL 261 Query: 215 KESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTR 274 P +VVGA + LE +G T+ + D S M + S + Sbjct: 260 TRVPEHLVVVGAGVIGLELGSVYKRLGAKVTV------VEYLDSACSGMDKGAVKSF*SC 99 Query: 275 FLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDT 306 + S + R G L+V W T ++T Sbjct: 98 *RNRASSSHLARKVTGFLEVNWRTWLTLHDET 3 >gb|ABWE01000605.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont52.3, whole genome shotgun sequence Length = 34187 Score = 54.3 bits (129), Expect = 5e-07 Identities = 37/118 (31%), Positives = 59/118 (50%) Frame = -2 Query: 385 GSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDAS 444 G ++Y +P ++T E+ VG +EEE A + +V + TVA D Sbjct: 34024 GKHGHVNYGAIPNVIYTFPEFASVGKTEEELKAEGVEYNVGKFPMMANSRARTVAEADGM 33845 Query: 445 QCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSE 502 VK++ ++ +L LG+H + NAGE+ +GI+ GA+ + RT HPT SE Sbjct: 33844 ---VKVLADKKTDKL-LGVHIIASNAGEMISEGVIGIEYGAASEDLARTCHAHPTLSE 33683 >gb|ABWE01000612.1| Hyaloperonospora parasitica strain Emoy2 v-7.0.1_Cont275.1, whole genome shotgun sequence Length = 33811 Score = 48.9 bits (115), Expect = 2e-05 Identities = 55/215 (25%), Positives = 85/215 (39%), Gaps = 17/215 (7%) Frame = -3 Query: 40 YDXXXXXXXXXXXACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQ 99 YD CA ++A+ G++VA+VD + TR LGG CV+ G IP K + Sbjct: 13823 YDLVVIGSGPSATQCALDSAKQGKRVAIVD------KKTR--LGGVCVHTGTIPSKTFRE 13668 Query: 100 AALLGGLIQDAPNYGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKA 159 A L + YG + + VQ V S R QL+ +V A Sbjct: 13667 AVLHLSGYRHHGFYGKSYSMKTV-TIEDILYRVQRVVSSEEDVVRAQLKAARVDVIPGFA 13491 Query: 160 SFVDEHTVCGVAKGGKE---------------ILLSADHIIIATGGRPRYPTHIEGALEY 204 F H + V +G + + AD ++A G RP + + + Sbjct: 13490 RFESAHEISIVKEGAAKDKAETGQCSNNMDARTRVRADKFLVACGTRPAHNPLVPIDGKI 13311 Query: 205 GITSDDIF--WLKESPGKTLVVGASYVALECAGFL 237 I SD I +++ P +VVGA + +E A + Sbjct: 13310 VIDSDQILKRGMQQLPRSLIVVGAGVIGMEYASMI 13206 Score = 36.2 bits (82), Expect = 0.13 Identities = 50/191 (26%), Positives = 80/191 (41%), Gaps = 10/191 (5%) Frame = -1 Query: 335 SPDTQKILVD---SREATSVPHIYAIGDVVEGRPELTPIAIMAGRLL------VQRLFGG 385 SP+T +L D S ++ + YA GD + G P L ++ GRL V G Sbjct: 12706 SPNTCVLLSDVYRSHDSAA*LSSYAAGDCI-GAPALASTSMEQGRLASCHMWEVDEEHAG 12530 Query: 386 SSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQ 445 +S L + N P ++T E VG +E++ + E+ A Y E + Sbjct: 12529 TSQL-ENGNYPYGIYTIPEISMVGQTEQQLTK--DCVNYEIGIAKYS--ELAKGQMMGAM 12365 Query: 446 CYVKMVCLREPPQL-VLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEV 504 + L +P L VLG+H +G A E+ + + G S V +PT +E Sbjct: 12364 AGGTLKILFDPDTLKVLGVHAIGEGATEIIHIGQVAMAMGCSLTYFRDAVFNYPTLAE-- 12191 Query: 505 VKLRISKRSGL 515 R++ +GL Sbjct: 12190 -AYRVAALNGL 12161 Database: H.arabidopsidis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 67,459,135 Number of sequences in database: 5422 Lambda K H 0.320 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 41,483,934 Number of Sequences: 5422 Number of extensions: 621590 Number of successful extensions: 2589 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 277 Number of HSP's successfully gapped in prelim test: 93 Number of HSP's that attempted gapping in prelim test: 2199 Number of HSP's gapped (non-prelim): 678 length of query: 524 length of database: 22,486,378 effective HSP length: 110 effective length of query: 414 effective length of database: 21,889,958 effective search space: 9062442612 effective search space used: 9062442612 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 66 (30.0 bits)