TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000034_1.0 # Protein # Thioredoxin reductase 1 (TR1) # Homo sapiens # Complete #U:498 (499 letters) Database: N.gruberi/genome.fa 784 sequences; 40,964,085 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG738881.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 435 e-122 gb|GG738854.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 235 e-106 gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 358 5e-99 gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 136 5e-32 gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 39 0.015 gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 37 0.034 gb|GG738887.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 29 9.2 gb|GG738851.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 29 9.2 gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 29 9.2 >gb|GG738881.1| Naegleria gruberi genomic scaffold NAEGRscaffold_37, whole genome shotgun sequence Length = 379849 Score = 435 bits (1119), Expect = e-122 Identities = 221/428 (51%), Positives = 295/428 (68%), Gaps = 7/428 (1%) Frame = -3 Query: 62 VGCIPKKLMHQAALLGQALQDSRNYGWK------VEETVKHDWDRMIEAVQNHIGSLNWG 115 VGCIPKKLMH AAL+G QD +GW E H W +++E VQ+HI LN+ Sbjct: 49346 VGCIPKKLMHTAALIGDRSQDGTAFGWTNSVGHGTERKPHHQWGKLVEMVQDHIHGLNFK 49167 Query: 116 YRVALREKKVVYENAYGQFIGPHRIKATNNKGKE-KIYSAERFLIATGERPRYLGIPGDK 174 YR LR+K + Y N Y FI H ++ + +E K ++A RF IA G RP Y IPG K Sbjct: 49166 YRTDLRKKGITYHNLYASFIDAHTVEGKKDGQEEGKRFTARRFAIAVGGRPLYPDIPGAK 48987 Query: 175 EYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMAN 234 EYCISSDD+FS+ PGK+LVVGASYVALEC GFL GIG D T+MVRS+ LRGFD++ A+ Sbjct: 48986 EYCISSDDIFSMEKEPGKSLVVGASYVALECGGFLHGIGNDTTIMVRSVPLRGFDRECAD 48807 Query: 235 KIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRD 294 KI M + G+KF + VP+ V ++E+ G+L V ++ ++E E++TV+ A GR Sbjct: 48806 KIVNVMSDEGVKFYQPCVPLSVNKLES---GKLSVEYENVETKEKKTEEFDTVLFATGRY 48636 Query: 295 ACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLA 354 A T+ + LE +GVK +K GKI V EQ++VP+IYAIGD++ +ELTPVAI+AG+LLA Sbjct: 48635 ALTQALNLENIGVK-TDKVGKIFVNKYEQSSVPHIYAIGDVIVGGLELTPVAIKAGKLLA 48459 Query: 355 QRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIP 414 RLY S DY+ VPTTVFTP+EYG+CGL+EE+AV+++G EN+ +Y ++ LE+ P Sbjct: 48458 ARLYNSSKKMMDYDKVPTTVFTPVEYGSCGLTEEEAVKRYGAENLTIYKKSYYVLEYEPP 48279 Query: 415 SRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPV 474 +R+ + + K+ICN DNERV+GFH +GPNAGEVTQGFA A++C TK+ D T+GIHP Sbjct: 48278 ARE-CQAFVKLICNELDNERVLGFHYVGPNAGEVTQGFAVAMRCNATKEDFDDTVGIHPT 48102 Query: 475 CAEVFTTL 482 CAE L Sbjct: 48101 CAETIVKL 48078 Score = 50.8 bits (120), Expect = 3e-06 Identities = 23/51 (45%), Positives = 27/51 (52%) Frame = -3 Query: 11 YDYDLIIIXXXXXXXXXXXXXXXXXXXVMVLDFVTPTPLGTRWGLGGTCVN 61 YDYDLI+I V V D++ P+P GT WGLGGTCVN Sbjct: 49709 YDYDLIVIGGGSGGIALAKEANALGAKVAVFDYIKPSPQGTTWGLGGTCVN 49557 >gb|GG738854.1| Naegleria gruberi genomic scaffold NAEGRscaffold_10, whole genome shotgun sequence Length = 665159 Score = 235 bits (599), Expect(3) = e-106 Identities = 130/307 (42%), Positives = 194/307 (63%), Gaps = 20/307 (6%) Frame = -2 Query: 50 GTRWGLGGTCVNVGCIPKKLMHQAALLGQALQ-DSRNYGW---KVEETV--KHDWDRMIE 103 G W LGGTCVNVGCIPKKLMH +AL+G+ + D+ +GW K E + KHDW ++ Sbjct: 89302 GWMWQLGGTCVNVGCIPKKLMHNSALIGEGINHDAEYFGWNDGKQEGKLSGKHDWKTLVA 89123 Query: 104 AVQNHIGSLNWGYRVALRE-----------KKVVYENAYGQFIGPHRIKATN-NKGKEKI 151 VQ +I SLN+GYR ++ + KK+ Y N G+F+ H ++ T+ KG K Sbjct: 89122 NVQQYIKSLNFGYRTSVTQTYAFDNEDPSLKKLYYLNMKGRFVDDHTLELTDPRKGITKT 88943 Query: 152 YSAERFLIATGERPRY-LGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLA 210 S + +++ G P +PG EY I+SDD+FSL PGKTLV+GASY+ALE A FL Sbjct: 88942 ISGKVIILSVGGTPNIPQDVPGATEYAITSDDIFSLKEEPGKTLVIGASYIALETASFLK 88763 Query: 211 GIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQ-IEAGTPGRLRV 269 G+G + +VM+RSI LRGFDQ+ A K+ + M+E G+KF+ Q +P+K+E+ + T G V Sbjct: 88762 GLGYESSVMMRSIPLRGFDQECAWKVVDGMKERGVKFLEQCIPVKIEKSSDHETTGEYTV 88583 Query: 270 VAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYI 329 + +++++ Y T+M A+GR A T+++ ++ +++GKI V+D EQT+ +I Sbjct: 88582 HYKDLSTQQVYTENYKTIMFAVGRRAVTKELNIDL----DMDESGKIIVSDNEQTSKKHI 88415 Query: 330 YAIGDIL 336 YAIGD + Sbjct: 88414 YAIGDCI 88394 Score = 140 bits (353), Expect(3) = e-106 Identities = 69/120 (57%), Positives = 89/120 (74%), Gaps = 1/120 (0%) Frame = -1 Query: 339 KVELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFT-PLEYGACGLSEEKAVEKFGEE 397 K ELTPVAI+AG+LLA RL T DY+NVPT++FT P EYG CGLSEE+AV+++GE+ Sbjct: 88343 KTELTPVAIRAGKLLASRLTNKGTELMDYDNVPTSIFTHPYEYGCCGLSEEEAVQRYGED 88164 Query: 398 NIEVYHSYFWPLEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALK 457 +IEVY S + P+E + RD NK + KII NE+V+GFH +G NAGE+TQG A A+K Sbjct: 88163 DIEVYISLYSPIEHQLSHRDTNKTFMKIITLKSANEKVIGFHYVGANAGEITQGIAVAIK 87984 Score = 50.8 bits (120), Expect(3) = e-106 Identities = 24/43 (55%), Positives = 29/43 (67%) Frame = -3 Query: 457 KCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQAGCUG 499 + G TK+ D+TIGIHP AE T LS+TKRSG S + GC G Sbjct: 87933 RAGATKEHFDNTIGIHPTAAEEMTLLSITKRSGNSAEKDGC*G 87805 >gb|GG738852.1| Naegleria gruberi genomic scaffold NAEGRscaffold_8, whole genome shotgun sequence Length = 938756 Score = 358 bits (920), Expect = 5e-99 Identities = 220/534 (41%), Positives = 301/534 (56%), Gaps = 56/534 (10%) Frame = +1 Query: 7 LPKSYDYDLIIIXXXXXXXXXXXXXXXXXXXVMVLDFVTPTPLGTRWGLGGTCVN----- 61 + Y YDLI++ V +LD+V P+ G+ W +GGTCVN Sbjct: 731812 MSSQYMYDLIVVGGGSGGLKCSKLAADLGAKVALLDYVKPSSQGSTWKIGGTCVNGM*WI 731991 Query: 62 ------------------------VGCIPKKLMHQAALLGQALQDSRNYGWKV------- 90 VGCIPKK MH A+ +G ++++ NYGWK+ Sbjct: 731992 D*LRWSTNDFNILYMHVCS*LK*SVGCIPKKQMHFASEMGNKIREAINYGWKLPLGLGEA 732171 Query: 91 -EETVKH-----------DWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPH 138 EE + + DW +++ V N N+ Y+ LR++K+ + A G+ + H Sbjct: 732172 PEEDMFNNFPHNKRGEFFDWTTLVQGVLNTRSGSNFVYKGELRKRKIDFYEALGRLVDSH 732351 Query: 139 RIKATNNKGKEKIYSAERFLIATGERPRY-LGIPGDKEYCISSDDLFSLPYCPGKTLVVG 197 ++ G+ + +A+ L+A G RP IPG EY I+SDDLFSL PGKTLVVG Sbjct: 732352 TVELV---GRVERLTAKYILLAPGGRPNVPRDIPGAYEYAITSDDLFSLQQAPGKTLVVG 732522 Query: 198 ASYVALECAGFLAGIGLDVTVMVRSILLR--GFDQDMANKIGEHME-EHGIKFIRQFVPI 254 SY+ALECAGFL+G+G DV+VMVRS +LR FD ++A+ + E ME H +KF+ +P+ Sbjct: 732523 GSYIALECAGFLSGLGFDVSVMVRSRILRSEAFDAEIASILRESMELHHHVKFLEPCIPV 732702 Query: 255 KVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTG 314 K+E++E + RV + + +++TVM A GR A + + L +GV+ + K G Sbjct: 732703 KIEKLE---NNQKRVTFLNATENKEYTQDFDTVMFATGRYADVQGLNLAAIGVQ-HTKEG 732870 Query: 315 KIPVTDEEQTNVPYIYAIGDILED--KVELTPVAIQAGRLLAQRLYAGSTV--KCDYENV 370 KI V DEE+T+V IYAIGD++E+ ELTPVAIQ G+ LA RL+ + K DY+ V Sbjct: 732871 KIIVNDEERTSVESIYAIGDVIENGHNYELTPVAIQQGKYLAYRLFKPEEINKKVDYDFV 733050 Query: 371 PTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKCYAKIICNTK 430 PTTVFTP EYG G SEEKA + FGE N+ +Y F LE I + K + K+IC Sbjct: 733051 PTTVFTPTEYGLVGYSEEKAKKVFGENNLVIYKKKFNILEHKI-AEIGEKGFVKLICVKN 733227 Query: 431 DNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSV 484 NERVVG H LGPNA EVTQGFA ALK G TK++ D IGIHP AE F L + Sbjct: 733228 QNERVVGLHYLGPNAAEVTQGFALALKKGCTKEEFDDVIGIHPSNAEAFMYLEL 733389 >gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_9, whole genome shotgun sequence Length = 695587 Score = 136 bits (342), Expect = 5e-32 Identities = 109/431 (25%), Positives = 204/431 (47%), Gaps = 3/431 (0%) Frame = +3 Query: 65 IPKKLMHQAALLGQALQDSRNYGWKVE-ETVKHDWDRMIEAVQNHIGSLNWGYRVALREK 123 +P K + A+ L + W + + + D M + + L G ++ Sbjct: 643518 VPSKALLHASHLYHEAHGTTFAKWGITMKDLNMDVSVMQKQKDKAVKGLTGGIEFLFKKY 643697 Query: 124 KVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFLIATGERPRYLG-IPGDKEYCISSDD 182 KV Y G G + + GK + + +IATG L +P D++ +SS Sbjct: 643698 KVEYVKGEGTVTGKNEVSVKGLDGKNSTIATDNIVIATGSEATPLPFLPFDEKVILSSTG 643877 Query: 183 LFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGF-DQDMANKIGEHME 241 +L + P + V+GA + LE + +G +VTV+ S + F D++++ + ME Sbjct: 643878 ALALDHVPKRMTVIGAGVIGLEMGSVYSRLGSEVTVIEYSDRISPFLDKEVSAVFQKTME 644057 Query: 242 EHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIG 301 + G+KF + V +K ++ G +L +V +T + E E + +++IGR T +G Sbjct: 644058 KLGMKF-KLGVSVKSGKVVDGKTCQLELVNNATGAAETFESD--VCLVSIGRRPHTANLG 644228 Query: 302 LETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGS 361 L VG+ ++E+ G++PV D +T+V IYAIGD++ + L A G +A++L AG Sbjct: 644229 LNNVGITLDER-GRVPVDDHFRTSVSNIYAIGDVVRGPM-LAHKAEDEGIAIAEQL-AGR 644399 Query: 362 TVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKC 421 + +Y+ +P ++T E + G +EE+ V+ + + + TI D + Sbjct: 644400 SGHVNYDAIPNVIYTHPEIASVGKTEEELVKAGIKYKVGKFPYSANSRARTID--DGTEG 644573 Query: 422 YAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTT 481 + KI+ + + ++V+G H++G AGE+ A++ G + + + T HP +E Sbjct: 644574 FVKILADAQ-TDKVLGVHIIGIYAGELIAEATLAMEYGASSEDIARTCHAHPTLSEAIKE 644750 Query: 482 LSVTKRSGASI 492 +V G I Sbjct: 644751 AAVAAYDGKPI 644783 >gb|GG738847.1| Naegleria gruberi genomic scaffold NAEGRscaffold_3, whole genome shotgun sequence Length = 941567 Score = 38.5 bits (88), Expect = 0.015 Identities = 17/29 (58%), Positives = 23/29 (79%), Gaps = 1/29 (3%) Frame = -2 Query: 366 DYENVPTTVFTP-LEYGACGLSEEKAVEK 393 DY+NV T++F EYG CGLSEE+AV++ Sbjct: 100420 DYDNVTTSIFNH*YEYGCCGLSEEEAVQR 100334 >gb|GG738906.1| Naegleria gruberi genomic scaffold NAEGRscaffold_62, whole genome shotgun sequence Length = 286411 Score = 37.4 bits (85), Expect = 0.034 Identities = 35/145 (24%), Positives = 64/145 (44%), Gaps = 3/145 (2%) Frame = +3 Query: 194 LVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVP 253 +V+GA Y+A E A F A G + VRS L+ D+++ + + K I Sbjct: 181326 IVIGAGYIATELAHFFASTGTKTNIFVRSGFLKHEDEEVQKEFDRVFSKK--KNINVIKG 181499 Query: 254 IKVEQIEAGTPGRLRVVAQSTNSEE---IIEGEYNTVMLAIGRDACTRKIGLETVGVKIN 310 +K+ ++A V+ S S+E IE +++A G T + + G++ + Sbjct: 181500 MKI--LKARYENDEFVLTYSNPSDESSGTIEVRAEGLLVAAGVVPNTDILQCDKAGIQTS 181673 Query: 311 EKTGKIPVTDEEQTNVPYIYAIGDI 335 + I + N P ++A GD+ Sbjct: 181674 KDGFIIVDKFLKSVNAPNVWAFGDV 181748 >gb|GG738887.1| Naegleria gruberi genomic scaffold NAEGRscaffold_43, whole genome shotgun sequence Length = 508457 Score = 29.3 bits (64), Expect = 9.2 Identities = 24/122 (19%), Positives = 54/122 (44%), Gaps = 4/122 (3%) Frame = +1 Query: 303 ETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGST 362 E+V + E+T + P+ ++++ + ++EDK E TPV ++ + + + + Sbjct: 18175 ESVVSEKKEETQETPIVEDKKE----VVIETPVVEDKKEETPVVVEETQPVVETVVEKKE 18342 Query: 363 VKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGE----ENIEVYHSYFWPLEWTIPSRDN 418 + E+ P +P+E ++ VE+ E EN+E + P++ + Sbjct: 18343 EETPIESTPAVTESPVETIIVEETQPVVVEEKKEEIVSENVETVVAETTPVQQEVTEVSE 18522 Query: 419 NK 420 K Sbjct: 18523 EK 18528 >gb|GG738851.1| Naegleria gruberi genomic scaffold NAEGRscaffold_7, whole genome shotgun sequence Length = 693645 Score = 29.3 bits (64), Expect = 9.2 Identities = 15/42 (35%), Positives = 25/42 (59%) Frame = +1 Query: 58 TCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWD 99 TC N+G +PK ++ +L ++L DS +V+E V DW+ Sbjct: 491602 TCFNIGALPKDILRDTEIL-RSLSDSILLNSEVQEYV--DWE 491718 >gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_1, whole genome shotgun sequence Length = 1555553 Score = 29.3 bits (64), Expect = 9.2 Identities = 24/100 (24%), Positives = 44/100 (44%), Gaps = 8/100 (8%) Frame = +1 Query: 92 ETVKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKI 151 E +K+D D ++EAV N S+ + + ++KKV+ G I + + +E++ Sbjct: 1252624 EALKNDKDVVMEAVSNSGQSIRYASKELRQDKKVIEAALKNDPYGVSYILSKLTE-EERL 1252800 Query: 152 YSAERFLIATGERPRY--------LGIPGDKEYCISSDDL 183 RF GE +Y L + D Y + + D+ Sbjct: 1252801 IGTWRFQFKVGEHSQYNHDLMIFDLVLNADYSYLLFNQDI 1252920 Database: N.gruberi/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 40,964,085 Number of sequences in database: 784 Lambda K H 0.319 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,598,432 Number of Sequences: 784 Number of extensions: 289675 Number of successful extensions: 1137 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 34 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 977 Number of HSP's gapped (non-prelim): 297 length of query: 499 length of database: 13,654,695 effective HSP length: 106 effective length of query: 393 effective length of database: 13,571,591 effective search space: 5333635263 effective search space used: 5333635263 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 64 (29.3 bits)