TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000034_1.0 # Protein # Thioredoxin reductase 1 (TR1) # Homo sapiens # Complete #U:498 (499 letters) Database: E.siliculosus/genome.fa 35 sequences; 195,934,119 total letters Searching...................................done Score E Sequences producing significant alignments: (bits) Value emb|FN649749.1| Ectocarpus siliculosus strain Ec 32, whole genom... 80 3e-14 emb|FN649740.1| Ectocarpus siliculosus strain Ec 32, whole genom... 76 4e-13 emb|FN649760.1| Ectocarpus siliculosus strain Ec 32, whole genom... 55 6e-07 emb|FN649750.1| Ectocarpus siliculosus strain Ec 32, whole genom... 44 0.001 emb|FN649726.1| Ectocarpus siliculosus strain Ec 32, whole genom... 44 0.002 emb|FN649748.1| Ectocarpus siliculosus strain Ec 32, whole genom... 35 1.0 emb|FN649742.1| Ectocarpus siliculosus strain Ec 32, whole genom... 32 8.7 >emb|FN649749.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG24 Length = 3847105 Score = 79.7 bits (195), Expect = 3e-14 Identities = 41/66 (62%), Positives = 50/66 (75%), Gaps = 1/66 (1%) Frame = -2 Query: 156 RFLIATGERPRYLG-IPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGL 214 R +IA G RP +PG +E+ ++SDDLFSL PGKTLVVGASY+ALECAGF+ +GL Sbjct: 2941779 RVIIAVGGRPVIPDDVPGAREHALTSDDLFSLRTSPGKTLVVGASYIALECAGFMRELGL 2941600 Query: 215 DVTVMV 220 DVTV V Sbjct: 2941599 DVTVRV 2941582 Score = 75.5 bits (184), Expect = 5e-13 Identities = 32/50 (64%), Positives = 38/50 (76%) Frame = -2 Query: 38 VMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYG 87 V++ DFV P+P GT+WGLGGTCVNVGC+PKKLMH A LLG+ N G Sbjct: 2944056 VVLFDFVKPSPQGTKWGLGGTCVNVGCVPKKLMHYAGLLGEGTYSMYNNG 2943907 Score = 55.8 bits (133), Expect = 4e-07 Identities = 26/68 (38%), Positives = 41/68 (60%) Frame = -1 Query: 58 TCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWDRMIEAVQNHIGSLNWGYR 117 T ++ GC+ L + G + D++ +GWKV +HDW+ M+E VQNH+ LN+ YR Sbjct: 2943646 TRLSWGCVGDSL---GVITGAGMHDAKAFGWKVGNP-EHDWEAMVETVQNHVKMLNFRYR 2943479 Query: 118 VALREKKV 125 V L+ +V Sbjct: 2943478 VGLKSAQV 2943455 Score = 55.8 bits (133), Expect = 4e-07 Identities = 25/44 (56%), Positives = 35/44 (79%) Frame = -3 Query: 218 VMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEA 261 V VRSILLRGFDQ +A K+G+ M++ G+KF+R VP K+E++ A Sbjct: 2941058 VAVRSILLRGFDQQVAEKLGDVMKDLGVKFVRPAVPSKIEKVRA 2940927 Score = 49.7 bits (117), Expect = 3e-05 Identities = 19/51 (37%), Positives = 33/51 (64%) Frame = -2 Query: 434 RVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSV 484 +V+GFH +GPNAGE+TQGF A++ G K + + +H + F+ +++ Sbjct: 2937261 KVIGFHFVGPNAGEITQGFGLAVRLGAKKSDFNKLVSMHSLPRASFSVVTL 2937109 Score = 42.7 bits (99), Expect = 0.004 Identities = 19/42 (45%), Positives = 27/42 (64%) Frame = -2 Query: 372 TTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTI 413 TT +EYG CGLSEE A+ +GE+++E Y F LE ++ Sbjct: 2939385 TTACWQVEYGCCGLSEEDAIRLYGEDDVETYLFGFGTLEQSV 2939260 Score = 42.0 bits (97), Expect = 0.006 Identities = 22/47 (46%), Positives = 31/47 (65%), Gaps = 1/47 (2%) Frame = -3 Query: 265 GRLRV-VAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKIN 310 G+L V + S + EE+ +G Y++V A GR A T IGLET GVK++ Sbjct: 2940668 GKLEVTLVDSKSGEEVSKGTYDSVFYATGRKADTSGIGLETAGVKVS 2940528 Score = 39.7 bits (91), Expect = 0.032 Identities = 19/32 (59%), Positives = 23/32 (71%) Frame = -2 Query: 348 QAGRLLAQRLYAGSTVKCDYENVPTTVFTPLE 379 QAG LLA RLYA +T + DY V T VFTP++ Sbjct: 2939787 QAGELLASRLYARATEQMDYSLVATAVFTPVK 2939692 Score = 38.9 bits (89), Expect = 0.054 Identities = 33/83 (39%), Positives = 42/83 (50%), Gaps = 14/83 (16%) Frame = -3 Query: 307 VKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKV----------ELTPVAIQAGR----L 352 V++N GKIPV + EQTNV +I+AIGD V ELTPVA+Q L Sbjct: 2940134 VQVNPN-GKIPV-ENEQTNVEHIFAIGDCTSVDVMFHESHWANPELTPVAVQVRSMLLLL 2939961 Query: 353 LAQRLYAGSTVKCDYENVPTTVF 375 L L+ G VP+T+F Sbjct: 2939960 LLLLLFCGV*SPYFSPPVPSTIF 2939892 Score = 32.7 bits (73), Expect = 3.9 Identities = 17/41 (41%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = -3 Query: 460 LTKKQLDSTIGIHPVCAEVFTTLSVTKRSGAS-ILQAGCUG 499 L K Q +GIHP AE F + +T+ SG S + + GC G Sbjct: 2936777 LGKPQQRL*VGIHPSDAESFCAMGITRSSGVSFVAEGGCGG 2936655 >emb|FN649740.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG15 Length = 5788546 Score = 75.9 bits (185), Expect = 4e-13 Identities = 34/65 (52%), Positives = 40/65 (61%) Frame = +3 Query: 8 PKSYDYDLIIIXXXXXXXXXXXXXXXXXXXVMVLDFVTPTPLGTRWGLGGTCVNVGCIPK 67 P YDYDL++I V LDFV P+ +G++WGLGGTCVNVGCIPK Sbjct: 911841 PSEYDYDLVVIGGGSGGLACAKEAARLGKKVACLDFVKPSVMGSKWGLGGTCVNVGCIPK 912020 Query: 68 KLMHQ 72 KLMHQ Sbjct: 912021 KLMHQ 912035 Score = 66.2 bits (160), Expect = 3e-10 Identities = 31/48 (64%), Positives = 37/48 (77%) Frame = +1 Query: 206 AGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVP 253 AGFL G+G+D TVMVRSILLRGFDQ +A +IG ME G +F+R VP Sbjct: 914500 AGFLTGLGVDTTVMVRSILLRGFDQGIAERIGSFMEAEGTRFLRPAVP 914643 Score = 63.2 bits (152), Expect = 3e-09 Identities = 35/66 (53%), Positives = 45/66 (68%) Frame = +3 Query: 148 KEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAG 207 +E++ S ++A G RP L G E ++SDD+F+L PGKTLVVGASYVALECAG Sbjct: 913863 QERVLSCGSVVVAVGGRPNQLPCEGG-ELAMTSDDIFALRTPPGKTLVVGASYVALECAG 914039 Query: 208 FLAGIG 213 AG+G Sbjct: 914040 -AAGVG 914054 Score = 49.7 bits (117), Expect = 3e-05 Identities = 24/47 (51%), Positives = 31/47 (65%) Frame = +3 Query: 318 VTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVK 364 V E+T V ++ IGD +E ELTP A+QAGRLLA RL+ G + K Sbjct: 916554 VCRNEETTVAGVFGIGDAVEGVPELTPSAVQAGRLLAMRLFGGESAK 916694 Score = 45.1 bits (105), Expect = 8e-04 Identities = 20/33 (60%), Positives = 24/33 (72%), Gaps = 1/33 (3%) Frame = +1 Query: 395 GEENIEVYHSYFWPLEWTIPS-RDNNKCYAKII 426 GEEN+EV+HS F PLEW + S R N CY K+I Sbjct: 917800 GEENVEVFHSAFTPLEWQMNSERPQNACYVKVI 917898 Score = 43.9 bits (102), Expect = 0.002 Identities = 27/76 (35%), Positives = 39/76 (51%), Gaps = 21/76 (27%) Frame = +2 Query: 71 HQAALLGQALQDSRNYGW----------KVEE-----------TVKHDWDRMIEAVQNHI 109 +QAALL +D+ +GW ++EE +V HDW+ M++ VQN+I Sbjct: 912284 NQAALLAGHAKDAPFFGWPAGPEGEDGSRLEEAGGVAGGEGKRSVTHDWEVMVQGVQNYI 912463 Query: 110 GSLNWGYRVALREKKV 125 LN+ YR LR K V Sbjct: 912464 KGLNFKYRTDLRSKGV 912511 Score = 40.4 bits (93), Expect = 0.019 Identities = 18/41 (43%), Positives = 23/41 (56%) Frame = +3 Query: 447 EVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKR 487 +V QGF ++ G+T L +GIHP AE T L TKR Sbjct: 920826 KVMQGFGVGIRLGMTMDDLRQLVGIHPTVAEELTLLQTTKR 920948 Score = 38.9 bits (89), Expect = 0.054 Identities = 15/24 (62%), Positives = 20/24 (83%) Frame = +2 Query: 426 ICNTKDNERVVGFHVLGPNAGEVT 449 +C+ D++RVVG H LGPNAGEV+ Sbjct: 920429 VCDLTDSQRVVGLHFLGPNAGEVS 920500 Score = 37.4 bits (85), Expect = 0.16 Identities = 19/36 (52%), Positives = 21/36 (58%) Frame = +2 Query: 360 GSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFG 395 GS DY NV TTVFTPL G G SE A++ G Sbjct: 917495 GSASAMDYSNVCTTVFTPLG*GGWGFSEGDAIKTMG 917602 >emb|FN649760.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, unassigned sequences LGUn Length = 58155991 Score = 55.5 bits (132), Expect = 6e-07 Identities = 24/49 (48%), Positives = 31/49 (63%) Frame = -1 Query: 434 RVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTL 482 +V+G HV+G A E+ QGF A+K G TK DS + IHP +E F TL Sbjct: 55096306 QVIGLHVIGEGADEMMQGFGVAMKLGATKADFDSCVAIHPTASEEFVTL 55096160 Score = 42.4 bits (98), Expect = 0.005 Identities = 26/72 (36%), Positives = 37/72 (51%), Gaps = 1/72 (1%) Frame = -2 Query: 190 PGKTLVVGASYVALECAGFLAGIGLDVTVMV-RSILLRGFDQDMANKIGEHMEEHGIKFI 248 P K V+GA Y+A+E AG +G D T++V R LR FD M + + M + G+ + Sbjct: 55098753 PKKVAVIGAGYIAVELAGVFQSLGTDTTLLVRRDRPLRSFDSLMVDTLMSEMGKSGLT-V 55098577 Query: 249 RQFVPIKVEQIE 260 R KV E Sbjct: 55098576 RPHTTTKVNSGE 55098541 Score = 38.9 bits (89), Expect = 0.054 Identities = 19/36 (52%), Positives = 25/36 (69%), Gaps = 1/36 (2%) Frame = -3 Query: 339 KVELTPVAIQAGRLLAQRLYAG-STVKCDYENVPTT 373 +V+LTP+AI AGR L+ RL+ G K DY NVP + Sbjct: 55097351 QVDLTPMAIAAGRRLSDRLFGGVDGAKADYNNVPVS 55097244 Score = 33.1 bits (74), Expect = 3.0 Identities = 14/31 (45%), Positives = 20/31 (64%) Frame = +3 Query: 55 LGGTCVNVGCIPKKLMHQAALLGQALQDSRN 85 +GGTCVN GC+P K + AA + +QD + Sbjct: 33015804 VGGTCVNRGCVPSKALLAAAGRVREMQDDHH 33015896 Score = 32.7 bits (73), Expect = 3.9 Identities = 14/48 (29%), Positives = 27/48 (56%) Frame = +3 Query: 422 YAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTI 469 YA + K+ E +VG H++G +A ++ Q + A+ G T ++L + Sbjct: 33021669 YAVQVLFNKETEEIVGVHIIGLHAADLIQECSNAVAAGTTVRELSMMV 33021812 Score = 31.6 bits (70), Expect = 8.7 Identities = 22/61 (36%), Positives = 31/61 (50%) Frame = +3 Query: 236 IGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDA 295 IGEH EH I+ +RQ V E +EAGT R V + + I + ++ +GRD Sbjct: 44788371 IGEH-REHCIQLLRQRV-FPSEVLEAGTKNREPVDVEVVHRVSISKLRMTPYVIHLGRDF 44788544 Query: 296 C 296 C Sbjct: 44788545 C 44788547 >emb|FN649750.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG25 Length = 4542878 Score = 44.3 bits (103), Expect = 0.001 Identities = 23/55 (41%), Positives = 30/55 (54%), Gaps = 3/55 (5%) Frame = -2 Query: 364 KCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPL---EWTIPS 415 K DY NVPT VF+ CGL+E A +GE+ ++VY S F L W + S Sbjct: 4067350 KADYTNVPTVVFSHPPMATCGLTEPDARAAYGEDAVKVYQSKFTNLFFGHWQVRS 4067186 Score = 42.0 bits (97), Expect = 0.006 Identities = 20/39 (51%), Positives = 29/39 (74%) Frame = -2 Query: 322 EQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAG 360 + T+ +YA+GD+ +VELTP+AI AGR L+ RL+AG Sbjct: 4067905 QNTSAEGVYALGDVT-GRVELTPMAIAAGRTLSDRLFAG 4067792 Score = 40.8 bits (94), Expect = 0.014 Identities = 19/33 (57%), Positives = 24/33 (72%), Gaps = 3/33 (9%) Frame = -3 Query: 56 GGTCVNVGCIPKKLMHQAALL---GQALQDSRN 85 GGTCVNVGC+PKK+M AA + G LQ+ R+ Sbjct: 4073367 GGTCVNVGCVPKKVMFNAAHINEVGAVLQERRH 4073269 Score = 40.4 bits (93), Expect = 0.019 Identities = 19/45 (42%), Positives = 28/45 (62%) Frame = -3 Query: 413 IPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALK 457 +P D K K+I T ++E+VVG H++G A E+ QGF A+K Sbjct: 4066302 MPPEDKEKTAMKVIV-TGESEKVVGIHIIGMGADEMMQGFGVAMK 4066171 Score = 35.4 bits (80), Expect = 0.60 Identities = 16/42 (38%), Positives = 23/42 (54%) Frame = -3 Query: 180 SDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVR 221 SD F + P K VVGA Y+A+E AG +G + + +R Sbjct: 4070184 SDGFFDVTEQPKKVAVVGAGYIAVELAGIFDALGTETHLFIR 4070059 >emb|FN649726.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG01 Length = 3745584 Score = 43.5 bits (101), Expect = 0.002 Identities = 18/52 (34%), Positives = 31/52 (59%) Frame = -1 Query: 426 ICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAE 477 I + K+ +R++G H+LGPNAGE+ A++ G + + + T HP +E Sbjct: 2341221 ILSDKETDRILGAHILGPNAGEMIAEVVLAMEYGASSEDIARTCHAHPTLSE 2341066 Score = 33.9 bits (76), Expect = 1.7 Identities = 16/33 (48%), Positives = 20/33 (60%) Frame = -1 Query: 51 TRWGLGGTCVNVGCIPKKLMHQAALLGQALQDS 83 +R LGGTC+NVGCIP K+ G + Q S Sbjct: 2345952 SRGKLGGTCLNVGCIPSKVWKVTGWHGVSCQFS 2345854 >emb|FN649748.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG23 Length = 3983565 Score = 34.7 bits (78), Expect = 1.0 Identities = 12/15 (80%), Positives = 15/15 (100%) Frame = -3 Query: 55 LGGTCVNVGCIPKKL 69 +GGTCVNVGC+PKK+ Sbjct: 2475604 MGGTCVNVGCVPKKV 2475560 >emb|FN649742.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG17 Length = 5807719 Score = 31.6 bits (70), Expect = 8.7 Identities = 16/38 (42%), Positives = 24/38 (63%) Frame = +1 Query: 266 RLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLE 303 RLR VA+S N E ++E NT+ A+ D+ RK+ L+ Sbjct: 102403 RLREVAES-NGERLLESSGNTISFAVTLDSLVRKVRLQ 102513 Database: E.siliculosus/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 195,934,119 Number of sequences in database: 35 Lambda K H 0.319 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 121,862,364 Number of Sequences: 35 Number of extensions: 1923290 Number of successful extensions: 8238 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 13 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 7394 Number of HSP's gapped (non-prelim): 3073 length of query: 499 length of database: 65,311,373 effective HSP length: 117 effective length of query: 382 effective length of database: 65,307,278 effective search space: 24947380196 effective search space used: 24947380196 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 70 (31.6 bits)