TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000034_1.0 # Protein # Thioredoxin reductase 1 (TR1) # Homo sapiens # Complete #U:498 (499 letters) Database: C.owczarzaki/genome.fa 89 sequences; 28,043,798 total letters Searching........................................................................................done Score E Sequences producing significant alignments: (bits) Value supercontig_1.2 of Capsaspora owczarzaki ATCC 30864 450 e-134 supercontig_1.13 of Capsaspora owczarzaki ATCC 30864 123 3e-28 supercontig_1.12 of Capsaspora owczarzaki ATCC 30864 29 6.4 >supercontig_1.2 of Capsaspora owczarzaki ATCC 30864 Length = 2668053 Score = 450 bits (1157), Expect(2) = e-134 Identities = 243/523 (46%), Positives = 310/523 (59%), Gaps = 103/523 (19%) Frame = +3 Query: 38 VMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALQ---------------- 81 V VLDFV P+P G+ WGLGGTCVNVGCIPKKLMHQA LLG+A++ Sbjct: 1734624 VCVLDFVKPSPQGSTWGLGGTCVNVGCIPKKLMHQACLLGEAIEVTKKARKGQPREAQAR 1734803 Query: 82 ------------------------------------DSRNYGWKVEET-VKHDWDRMIEA 104 D+ +GW+VE+ +KHDW +++ Sbjct: 1734804 AHQTSIEVF*LEHAKTTQTP*LRKPLF*TLV*LAL*DAEKFGWEVEKANIKHDWSKLVMG 1734983 Query: 105 VQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFLIATGER 164 V +HIG LN+ YRV+LRE V Y NAYG F H I+ T+ K K +AE F++A G R Sbjct: 1734984 VSDHIGGLNFAYRVSLRENNVTYLNAYGVFKDAHTIECTDKKRKVTTLTAEHFVLAVGGR 1735163 Query: 165 PRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSIL 224 P+Y IPG +E I+SDD+FSLPY PGKTLVVGASYVALECAGFL G+G D TVM RSI Sbjct: 1735164 PKYPDIPGARELGITSDDMFSLPYAPGKTLVVGASYVALECAGFLTGLGYDTTVMARSIF 1735343 Query: 225 LRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIE-----AGTPGRLRVVAQSTNSEEI 279 LRGFDQ A +I +M EHG KF+R+ VP+ +E+IE A R+RV + + E Sbjct: 1735344 LRGFDQQCAEQIAAYMSEHGTKFLRRCVPLSLERIEPEADSATGRHRIRVTWEDGLANEP 1735523 Query: 280 IEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDK 339 ++TV+ AIGR+A T IGLE +G+K N +GK+ EQT+VP ++AIGD++ + Sbjct: 1735524 GSDVFDTVLFAIGREAVTSTIGLEALGLKAN-ASGKVD-APLEQTSVPNVFAIGDVINGR 1735697 Query: 340 VELTPVAIQ---------------------------------------------AGRLLA 354 ELTPVAIQ AGRLLA Sbjct: 1735698 PELTPVAIQVRVWSSSFAREGLLHSGLCCRGLIPSLPLPRLLPIIIIFSHDDA*AGRLLA 1735877 Query: 355 QRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIP 414 +RLY G+T + DY N+PTTVFTPLEYG G++EE A+ + EENIEV+H YF PLEWT+ Sbjct: 1735878 KRLYGGATTQMDYANIPTTVFTPLEYGCIGMAEEDAIALYKEENIEVFHQYFQPLEWTVA 1736057 Query: 415 SRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALK 457 R + CY K+ICN DN RV+G H+LGPNAGE+TQG+ A + Sbjct: 1736058 HRPASTCYCKLICNKLDNMRVIGLHILGPNAGEITQGYGVAFR 1736186 Score = 168 bits (426), Expect = 7e-42 Identities = 131/417 (31%), Positives = 199/417 (47%), Gaps = 67/417 (16%) Frame = -2 Query: 133 QFIGPHRIKATNNKGKEKIYSAERFLIATGER----------------------PRY--- 167 +F+GP ++ KIYSA+ LIATG PRY Sbjct: 397985 RFVGPKQVSVDG-----KIYSAKHILIATGSYAKVCVQVYAQPFFYRCLFC*LVPRYSSW 397821 Query: 168 LG--------IPGDKEYCISSDDLFSLPYCPGKTLVVGASYV------------------ 201 LG +PG + I+SD F L P K VVGA YV Sbjct: 397820 LGLRCAQLPDVPG-AHHGITSDGFFELNEQPKKVAVVGAGYVPVFFFGGGLHYLKFEQSV 397644 Query: 202 --------------ALECAGFLAGIGLDVTVMVRSI-LLRGFDQDMANKIGEHMEEHGIK 246 A+E AG +G +V+++VR +LR FD + + E + G+ Sbjct: 397643 RTDIQMRHT*SRYIAVELAGIFRALGSEVSLLVRHDGVLRKFDSMLHKGVEEELTASGVN 397464 Query: 247 FIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVG 306 + + V + G L + + ++ ++ ++ AIGR + LE G Sbjct: 397463 LL---LNTHVSSVTKDDSGHLSLALTNGTTQS----GFDCLVWAIGRSPTIEDLDLEKTG 397305 Query: 307 VKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGST-VKC 365 V + +K G I V + + T I+A+GD+ +LTPVAI AGR L++RL+AG T +K Sbjct: 397304 VSL-DKDGLIKVDEFQNTTHEGIHAVGDVASHW-QLTPVAIAAGRRLSERLFAGKTDLKL 397131 Query: 366 DYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKCYAKI 425 +YEN+ + VF+ G GLSEE A+ K G++N+++Y + F + ++ +R K K+ Sbjct: 397130 EYENIASVVFSHPALGTVGLSEEDAIAKHGKDNLKIYSATFTNMYHSMTTR-KTKTRVKL 396954 Query: 426 ICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTL 482 +C K+ E+V+G HV+G A E+ QGFA A+K G TK Q D T+ IHP +E TL Sbjct: 396953 VCLGKE-EKVIGVHVIGIGADEMIQGFAVAVKMGATKAQFDDTVAIHPTASEELVTL 396786 Score = 47.0 bits (110), Expect(2) = e-134 Identities = 21/41 (51%), Positives = 25/41 (60%) Frame = +2 Query: 459 GLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQAGCUG 499 G TK D T+GIHP C+E FTTL +T+ SG GC G Sbjct: 1736276 GATKDDFDMTVGIHPTCSENFTTLDITRASGLDPGAKGC*G 1736398 Score = 39.7 bits (91), Expect = 0.005 Identities = 19/36 (52%), Positives = 26/36 (72%) Frame = -2 Query: 60 VNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVK 95 VNVGC+PKK+M A + +AL D+ +YG+ V TVK Sbjct: 398453 VNVGCVPKKVMWNTASMAEALHDAPDYGFDV--TVK 398352 >supercontig_1.13 of Capsaspora owczarzaki ATCC 30864 Length = 955311 Score = 123 bits (308), Expect = 3e-28 Identities = 98/357 (27%), Positives = 169/357 (47%), Gaps = 5/357 (1%) Frame = -1 Query: 92 ETVKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKI 151 E VK D +++ A + L G + L++ KV Y +G+ G + G ++ Sbjct: 534756 ENVKLDLPKLMAAKDKAVTGLTGGVEMLLKKNKVDYVKGHGKITGAQEVTVDLLGGGSQV 534577 Query: 152 YSAERFLIATG-ERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLA 210 A+R LIATG E + GI D++ +SS +L P K +V+G + LE + Sbjct: 534576 LKAKRILIATGSEVTPFPGIAIDEQTIVSSTGALALKAVPKKMIVIGGGVIGLELGSVWS 534397 Query: 211 GIGLDVTVM--VRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLR 268 +G +VTV+ + I G D ++A + + G+ F + KV G ++ Sbjct: 534396 RLGAEVTVVEFLGGIGGAGIDGEVAKNFQRVLTKQGLNF---KLNTKVTGATKQANGSVK 534226 Query: 269 VVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPY 328 V +S + + + +++AIGR T +GL+ GVK++ + G++ V + TNVP Sbjct: 534225 VNVESADGSVKETLDADVLLVAIGRRPYTNNLGLKEAGVKVDNR-GRVEVNNMFTTNVPS 534049 Query: 329 IYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEE 388 IYAIGD+++ + L A G +LA G DY NVP+ ++T E G +EE Sbjct: 534048 IYAIGDVIQGPM-LAHKAEDEG-ILAVEGMCGGHPHIDYNNVPSVIYTHPEVAWVGKTEE 533875 Query: 389 KAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKC--YAKIICNTKDNERVVGFHVLGP 443 + E I + P ++ NN + K++ K ++++G H++GP Sbjct: 533874 QLKADGVEYTIGKF-----PFVANSRAKTNNDTDGFVKVL-GDKKTDQILGAHIIGP 533722 Score = 35.4 bits (80), Expect = 0.089 Identities = 18/37 (48%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = -3 Query: 52 RWGLGGTCVNVGCIPKK-LMHQAALLGQALQDSRNYG 87 R LGGTC+NVGCIP K L++ + A D N G Sbjct: 534997 RGALGGTCLNVGCIPSKALLNNSHYYHMAQHDFANRG 534887 Score = 30.8 bits (68), Expect = 2.2 Identities = 46/220 (20%), Positives = 89/220 (40%), Gaps = 18/220 (8%) Frame = -2 Query: 165 PRYLGIPGDKEYCI----SSDD--LFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTV 218 PR L +PG + S +D F+ ++VG+S++ +E A +A VTV Sbjct: 504881 PRILNVPGSTSKGVHVLRSPEDSAAFAADAENKNVVIVGSSFIGMEAAAAIAKKAASVTV 504702 Query: 219 M-VRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSE 277 + + + R F + + + + ++ IK + +K I G G + V + + Sbjct: 504701 LGLEAPFSRVFGEKVGAAVAKLHTDNNIKIVTSAAGVK--SINTGADGGITGVELADGT- 504531 Query: 278 EIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPV-----------TDEEQTNV 326 ++ G+ V+ +G T+ + GV +N G I V + Sbjct: 504530 -VLPGQ--VVLFGVGVQLNTQFV-KAGAGVTVN-PDGSISVDKFGHAGNGLYVAGDVARF 504366 Query: 327 PYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVKCD 366 PY Y+ ++E VA + G ++ + + AG + D Sbjct: 504365 PYKYSASGSAAVRIEHFQVAERLGSIVGKNI-AGKATEID 504249 >supercontig_1.12 of Capsaspora owczarzaki ATCC 30864 Length = 1052152 Score = 29.3 bits (64), Expect = 6.4 Identities = 16/63 (25%), Positives = 25/63 (39%) Frame = -1 Query: 53 WGLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWDRMIEAVQNHIGSL 112 W C + C + Q LL + + R W+ K+ R I A H+ S+ Sbjct: 471046 WLYSRCCKRLCCSEAPEILQTNLLSRRILVKRKCLWRTPCECKYSIPRAISAAIEHLKSM 470867 Query: 113 NWG 115 +WG Sbjct: 470866 SWG 470858 Database: C.owczarzaki/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 28,043,798 Number of sequences in database: 89 Lambda K H 0.319 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,226,111 Number of Sequences: 89 Number of extensions: 240720 Number of successful extensions: 1083 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 978 Number of HSP's gapped (non-prelim): 215 length of query: 499 length of database: 9,347,932 effective HSP length: 104 effective length of query: 395 effective length of database: 9,338,676 effective search space: 3688777020 effective search space used: 3688777020 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 62 (28.5 bits)