TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fep15_sal selenoprotein [Salmo salar] (137 letters) Database: T.trahens/genome.fa 131 sequences; 28,680,627 total letters Searching.................................................................done Score E Sequences producing significant alignments: (bits) Value gb|GL349464.1| Thecamonas trahens ATCC 50062 unplaced genomic sc... 28 2.3 >gb|GL349464.1| Thecamonas trahens ATCC 50062 unplaced genomic scaffold supercont1.32, whole genome shotgun sequence Length = 401146 Score = 27.7 bits (60), Expect = 2.3 Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 4/48 (8%) Frame = +3 Query: 56 FLMERWALYHNLEYDSGEEKNPRLIF----YDDKDEVVQTVPVKKMKV 99 +L++ W + L S + ++ RL F DDK E+V VPV + KV Sbjct: 386847 WLIDGWEPFGELGIGSWDSESSRLSFDVTEKDDKYEIVAHVPVPRDKV 386990 Database: T.trahens/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 28,680,627 Number of sequences in database: 131 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,720,466 Number of Sequences: 131 Number of extensions: 35350 Number of successful extensions: 110 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 109 Number of HSP's gapped (non-prelim): 5 length of query: 137 length of database: 9,560,209 effective HSP length: 89 effective length of query: 48 effective length of database: 9,548,550 effective search space: 458330400 effective search space used: 458330400 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 55 (25.8 bits)