TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fep15_sal selenoprotein [Salmo salar] (137 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 28 1.5 >gb|CM000611.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 8, whole genome shotgun sequence Length = 1007773 Score = 28.1 bits (61), Expect = 1.5 Identities = 23/87 (26%), Positives = 40/87 (45%), Gaps = 6/87 (6%) Frame = +3 Query: 44 GUAIKKMPALYMFLME---RWALYHNLEYDSGEEKNPRLIFYDD---KDEVVQTVPVKKM 97 G + +MP L FL + W + N+ Y +G + + Y++ K EV+ K Sbjct: 7548 G*MLNRMPELKSFLKDGEAEWYVGVNVTYVTG--RRAVMHIYNEGGSKVEVIDLTVSKLQ 7721 Query: 98 KVDEISSLLDSLGFYKRSQKGEEVPEE 124 + +L+ F KRS++ E+P E Sbjct: 7722 TKKALHALMAEEEFVKRSEEAVEMPHE 7802 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.318 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,113,581 Number of Sequences: 31 Number of extensions: 34090 Number of successful extensions: 142 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 0 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 142 Number of HSP's gapped (non-prelim): 1 length of query: 137 length of database: 7,911,228 effective HSP length: 87 effective length of query: 50 effective length of database: 7,908,531 effective search space: 395426550 effective search space used: 395426550 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)