TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fep15_sal selenoprotein [Salmo salar]
         (137 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738905.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   5.4  
gb|GG738926.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    26   9.1  

>gb|GG738905.1| Naegleria gruberi genomic scaffold NAEGRscaffold_61, whole genome shotgun
              sequence
          Length = 290827

 Score = 26.9 bits (58), Expect = 5.4
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = -2

Query: 68     EYDSGEEKNPRLIFYDDKDE 87
              EYD  EE++  ++ YDDK++
Sbjct: 287613 EYDGEEEEDEDIVIYDDKED 287554


>gb|GG738926.1| Naegleria gruberi genomic scaffold NAEGRscaffold_82, whole genome
             shotgun sequence
          Length = 174974

 Score = 26.2 bits (56), Expect = 9.1
 Identities = 9/32 (28%), Positives = 17/32 (53%)
 Frame = -2

Query: 63    LYHNLEYDSGEEKNPRLIFYDDKDEVVQTVPV 94
             +YH + +    +   ++I YDDK  V+   P+
Sbjct: 26881 IYHEMSFGDETQAIAKMIVYDDKSNVMLMQPI 26786


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.318    0.136    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,287,236
Number of Sequences: 784
Number of extensions: 59228
Number of successful extensions: 300
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 241
Number of HSP's gapped (non-prelim): 116
length of query: 137
length of database: 13,654,695
effective HSP length: 91
effective length of query: 46
effective length of database: 13,583,351
effective search space: 624834146
effective search space used: 624834146
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)