TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fep15_ory selenoprotein [Oryzias latipes] (130 letters) Database: S.parasitica/genome.fa 1445 sequences; 53,132,636 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG743888.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 28 2.0 gb|GG743932.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 28 3.4 gb|GG743885.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 27 7.7 >gb|GG743888.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.6, whole genome shotgun sequence Length = 961754 Score = 28.5 bits (62), Expect = 2.0 Identities = 27/108 (25%), Positives = 46/108 (42%), Gaps = 5/108 (4%) Frame = -1 Query: 26 ARG---KLMAPSVVGUAIKKLPELNRFLMERWALYHNLEYDSSEEKNPRLI--LYNEKDE 80 ARG +L+ S+VG +++ L L W++Y N + + + +E Sbjct: 797621 ARGVKHELLQESIVGLRPRRVFPLRHRLENNWSIYINKKMGRGSNLLDVFVARAVEDPEE 797442 Query: 81 VVKTVPVKEMTADEISNLLDSLGFYKRSKKGEEVPEEFQHFPLRTPRD 128 P +E A+E LL+ + K+ E PE H P++ RD Sbjct: 797441 EDDAAPEREEEAEE---LLEHAVACREPKRHVETPE---HHPVQNERD 797316 >gb|GG743932.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.50, whole genome shotgun sequence Length = 253698 Score = 27.7 bits (60), Expect = 3.4 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -3 Query: 50 LMERWALYHNLEYDSSEEKNPRLILYNEKDEVVKTVP 86 L E+W LYH+L + S E + PRL + D + P Sbjct: 105904 LFEQWTLYHSL-FHSFEMRCPRLEVIKLADAAIVRAP 105797 >gb|GG743885.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.3, whole genome shotgun sequence Length = 1275006 Score = 26.6 bits (57), Expect = 7.7 Identities = 11/35 (31%), Positives = 20/35 (57%) Frame = +1 Query: 40 IKKLPELNRFLMERWALYHNLEYDSSEEKNPRLIL 74 +K++P +R L W+ + YD++ + RLIL Sbjct: 492319 LKQVPSTSRVLKRGWSTRTS*RYDAASRRKRRLIL 492423 Database: S.parasitica/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 53,132,636 Number of sequences in database: 1445 Lambda K H 0.316 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,222,221 Number of Sequences: 1445 Number of extensions: 57489 Number of successful extensions: 264 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 32 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 217 Number of HSP's gapped (non-prelim): 88 length of query: 130 length of database: 17,710,878 effective HSP length: 91 effective length of query: 39 effective length of database: 17,579,383 effective search space: 685595937 effective search space used: 685595937 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 56 (26.2 bits)