TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fep15_ory selenoprotein [Oryzias latipes] (130 letters) Database: P.ultimum/genome.fa 82 sequences; 42,801,618 total letters Searching.................................................................................done Score E Sequences producing significant alignments: (bits) Value gb|GL376564.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 27 3.7 gb|GL376560.1| Pythium ultimum DAOM BR144 unplaced genomic scaff... 27 6.4 >gb|GL376564.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581313, whole genome shotgun sequence Length = 1115623 Score = 27.3 bits (59), Expect = 3.7 Identities = 20/64 (31%), Positives = 27/64 (42%) Frame = +3 Query: 56 LYHNLEYDSSEEKNPRLILYNEKDEVVKTVPVKEMTADEISNLLDSLGFYKRSKKGEEVP 115 LY E +N + EK EVV + + + +L LGF+KR GE P Sbjct: 600972 LYQRCERAVDNGENHFTLRRFEKREVVLRAFNERGEHESLVRVLVDLGFWKRQTPGEGSP 601151 Query: 116 EEFQ 119 FQ Sbjct: 601152 IGFQ 601163 >gb|GL376560.1| Pythium ultimum DAOM BR144 unplaced genomic scaffold scf_1117875581242, whole genome shotgun sequence Length = 870537 Score = 26.6 bits (57), Expect = 6.4 Identities = 10/24 (41%), Positives = 17/24 (70%) Frame = +2 Query: 106 KRSKKGEEVPEEFQHFPLRTPRDE 129 +R GEE+ ++F+ P++TPR E Sbjct: 402929 ERKDAGEEMKKKFRVMPVQTPRSE 403000 Database: P.ultimum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 42,801,618 Number of sequences in database: 82 Lambda K H 0.316 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,993,498 Number of Sequences: 82 Number of extensions: 47173 Number of successful extensions: 252 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 249 Number of HSP's gapped (non-prelim): 11 length of query: 130 length of database: 14,267,206 effective HSP length: 90 effective length of query: 40 effective length of database: 14,259,826 effective search space: 570393040 effective search space used: 570393040 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)