TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fep15_ory selenoprotein [Oryzias latipes] (130 letters) Database: E.siliculosus/genome.fa 35 sequences; 195,934,119 total letters Searching...................................done Score E Sequences producing significant alignments: (bits) Value emb|FN649756.1| Ectocarpus siliculosus strain Ec 32, whole genom... 32 0.41 >emb|FN649756.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG31 Length = 577967 Score = 32.3 bits (72), Expect = 0.41 Identities = 15/38 (39%), Positives = 21/38 (55%) Frame = +2 Query: 54 WALYHNLEYDSSEEKNPRLILYNEKDEVVKTVPVKEMT 91 WAL +E D + + +P L +K E + TVPVK T Sbjct: 356285 WALSERVEIDCARDSDPDLRRPRQKHERIPTVPVKRQT 356398 Database: E.siliculosus/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 195,934,119 Number of sequences in database: 35 Lambda K H 0.316 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 23,483,643 Number of Sequences: 35 Number of extensions: 228063 Number of successful extensions: 932 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 919 Number of HSP's gapped (non-prelim): 38 length of query: 130 length of database: 65,311,373 effective HSP length: 99 effective length of query: 31 effective length of database: 65,307,908 effective search space: 2024545148 effective search space used: 2024545148 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 60 (27.7 bits)