TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fep15_onc selenoprotein [Oncorhynchus mykiss] (137 letters) Database: S.parasitica/genome.fa 1445 sequences; 53,132,636 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG744089.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 28 3.0 gb|GG743962.1| Saprolegnia parasitica CBS 223.65 genomic scaffol... 27 6.8 >gb|GG744089.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.207, whole genome shotgun sequence Length = 65165 Score = 28.1 bits (61), Expect = 3.0 Identities = 11/20 (55%), Positives = 17/20 (85%) Frame = -2 Query: 85 KDEVVQTVPVKKMKVDEISS 104 +DE+VQT+PVK +++D SS Sbjct: 38719 EDEIVQTLPVKSLEIDCASS 38660 >gb|GG743962.1| Saprolegnia parasitica CBS 223.65 genomic scaffold supercont1.80, whole genome shotgun sequence Length = 147863 Score = 26.9 bits (58), Expect = 6.8 Identities = 21/80 (26%), Positives = 36/80 (45%), Gaps = 2/80 (2%) Frame = +2 Query: 17 TEENVTDTAIEERPVIAKGILKAPSVVGUAIKKMPELYTFLMERWALYHNLEYD--SGEE 74 T++ T T V A ++++ S ++ K+ +Y RW H L YD +G + Sbjct: 73502 TQKKRTSTQKNMAAVRAMPLVRSQSCSVASV*KLE*IYEHGRRRWHQLHQLAYDAEAGRD 73681 Query: 75 KNPRLIFYDDKDEVVQTVPV 94 + R KDE ++T V Sbjct: 73682 FHAR-----QKDEPIETAVV 73726 Database: S.parasitica/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 53,132,636 Number of sequences in database: 1445 Lambda K H 0.318 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,837,345 Number of Sequences: 1445 Number of extensions: 68741 Number of successful extensions: 274 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 35 Number of HSP's successfully gapped in prelim test: 7 Number of HSP's that attempted gapping in prelim test: 230 Number of HSP's gapped (non-prelim): 69 length of query: 137 length of database: 17,710,878 effective HSP length: 92 effective length of query: 45 effective length of database: 17,577,938 effective search space: 791007210 effective search space used: 791007210 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 57 (26.6 bits)