TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fep15_onc selenoprotein [Oncorhynchus mykiss]
         (137 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    29   1.1  
gb|GG738926.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   5.4  
gb|GG738905.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   5.4  
gb|GG738864.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   7.0  
gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    26   9.1  

>gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_1, whole genome shotgun
              sequence
          Length = 1555553

 Score = 29.3 bits (64), Expect = 1.1
 Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 1/75 (1%)
 Frame = +3

Query: 51     PELYTFLMERWALYHNLEYDSGEEKNPRLIFYDDKDEVVQTVPVKK-MKVDEISSLLDSL 109
              PEL   ++E+      L Y+S  + N  L  +DD D      PV + +    +  + D +
Sbjct: 698862 PELKLKILEKKKKQEQLFYNSLRKVNAFLFNFDDLDRETGCSPVHQILDYSSLRLIFDKM 699041

Query: 110    GFYKRSQKGEEVPKE 124
              G Y  S   E +  E
Sbjct: 699042 GIYPISASTESLTDE 699086


>gb|GG738926.1| Naegleria gruberi genomic scaffold NAEGRscaffold_82, whole genome
             shotgun sequence
          Length = 174974

 Score = 26.9 bits (58), Expect = 5.4
 Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = -2

Query: 52    ELYTFL-MERWALYHNLEYDSGEEKNPRLIFYDDKDEVVQTVPV 94
             ELY  + ++   +YH + +    +   ++I YDDK  V+   P+
Sbjct: 26917 ELYRSISVQNRIIYHEMSFGDETQAIAKMIVYDDKSNVMLMQPI 26786



 Score = 26.6 bits (57), Expect = 7.0
 Identities = 14/55 (25%), Positives = 29/55 (52%)
 Frame = +3

Query: 46    AIKKMPELYTFLMERWALYHNLEYDSGEEKNPRLIFYDDKDEVVQTVPVKKMKVD 100
             ++K+M E ++ L++          DS  +K  R   +DD ++ + T+ +KK  +D
Sbjct: 37683 SMKEMEEFFSALLKT--------LDSTNDKGDRATMFDDIEQNLNTMKIKKKILD 37823


>gb|GG738905.1| Naegleria gruberi genomic scaffold NAEGRscaffold_61, whole genome shotgun
              sequence
          Length = 290827

 Score = 26.9 bits (58), Expect = 5.4
 Identities = 9/20 (45%), Positives = 15/20 (75%)
 Frame = -2

Query: 68     EYDSGEEKNPRLIFYDDKDE 87
              EYD  EE++  ++ YDDK++
Sbjct: 287613 EYDGEEEEDEDIVIYDDKED 287554


>gb|GG738864.1| Naegleria gruberi genomic scaffold NAEGRscaffold_20, whole genome shotgun
              sequence
          Length = 441990

 Score = 26.6 bits (57), Expect = 7.0
 Identities = 15/46 (32%), Positives = 25/46 (54%)
 Frame = -2

Query: 36     ILKAPSVVGUAIKKMPELYTFLMERWALYHNLEYDSGEEKNPRLIF 81
              +LK  + +   I++ P+LYT+L E+     N +      KNPR+ F
Sbjct: 407066 VLKMATDLKNLIERYPQLYTYLEEKEL---NSQSPLALPKNPRMYF 406938


>gb|GG738860.1| Naegleria gruberi genomic scaffold NAEGRscaffold_16, whole genome shotgun
              sequence
          Length = 536960

 Score = 26.2 bits (56), Expect = 9.1
 Identities = 17/70 (24%), Positives = 36/70 (51%), Gaps = 1/70 (1%)
 Frame = +3

Query: 41     SVVGUAIKKMPELYTFLMERWALYHNLEYDSGEEKNPRLIFYDDKDEV-VQTVPVKKMKV 99
              ++V   +K+MP +YT L++  ++  N           RLIF  +  EV V++     + +
Sbjct: 302472 TIVEIILKEMPHMYTLLLQILSIMKN-----------RLIFDSEATEVHVRSKQQLSLWL 302618

Query: 100    DEISSLLDSL 109
              +E++ L+  +
Sbjct: 302619 EEVTDLISKV 302648


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.318    0.136    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,734,858
Number of Sequences: 784
Number of extensions: 64367
Number of successful extensions: 307
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 240
Number of HSP's gapped (non-prelim): 114
length of query: 137
length of database: 13,654,695
effective HSP length: 91
effective length of query: 46
effective length of database: 13,583,351
effective search space: 624834146
effective search space used: 624834146
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)