TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fep15_dre selenoprotein [Danio rerio]
         (138 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738878.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    29   1.4  
gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   4.2  
gb|GG738921.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   5.5  
gb|GG738865.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   5.5  
gb|GG738895.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   7.1  
gb|GG738892.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   7.1  
gb|GG738856.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   7.1  

>gb|GG738878.1| Naegleria gruberi genomic scaffold NAEGRscaffold_34, whole genome shotgun
              sequence
          Length = 395411

 Score = 28.9 bits (63), Expect = 1.4
 Identities = 16/54 (29%), Positives = 29/54 (53%)
 Frame = -3

Query: 72     SGEDKNPRLIFYNDKDEEVKVVPVKKMKADEISSLLDSLGFYKRSEKGEEVPEE 125
              +G ++   +    +     K   +  +KA E+++LLDSLG  K +EK  ++ EE
Sbjct: 306858 TGNERGLIINLIKNNHPSFKAGKMTNIKASEMNALLDSLGITKFTEKWFKLIEE 306697


>gb|GG738846.1| Naegleria gruberi genomic scaffold NAEGRscaffold_2, whole genome shotgun
              sequence
          Length = 1001995

 Score = 27.3 bits (59), Expect = 4.2
 Identities = 19/57 (33%), Positives = 27/57 (47%)
 Frame = -3

Query: 75     DKNPRLIFYNDKDEEVKVVPVKKMKADEISSLLDSLGFYKRSEKGEEVPEEFKHFPL 131
              D++   I Y   DEEV  +  K     + SSL D+L   K   +  E P + K FP+
Sbjct: 202622 DEDESNIEYYTADEEVHTIAKK-----QFSSLSDNLHIKKHKIQVIEDPNDCKEFPV 202467


>gb|GG738921.1| Naegleria gruberi genomic scaffold NAEGRscaffold_77, whole genome
             shotgun sequence
          Length = 207235

 Score = 26.9 bits (58), Expect = 5.5
 Identities = 12/36 (33%), Positives = 22/36 (61%)
 Frame = -1

Query: 67    NLEYDSGEDKNPRLIFYNDKDEEVKVVPVKKMKADE 102
             NL     EDK+ +++  NDK +++  +P  ++KA E
Sbjct: 63793 NLMKKEKEDKDEQIVSLNDKIKKLSTIPKTQIKALE 63686


>gb|GG738865.1| Naegleria gruberi genomic scaffold NAEGRscaffold_21, whole genome shotgun
              sequence
          Length = 820293

 Score = 26.9 bits (58), Expect = 5.5
 Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
 Frame = +3

Query: 48     IKKMPELYNF--LMERWALYHNLEYDSGEDKNPRLIFYNDKDEEVKVV 93
              ++K+PE Y+   L + +     L+ DSG   NP +IF   + + +++V
Sbjct: 130899 LEKIPEEYDTKELKKFYKKLETLKKDSGGANNPMIIFLKQEIDRMRIV 131042


>gb|GG738895.1| Naegleria gruberi genomic scaffold NAEGRscaffold_51, whole genome
             shotgun sequence
          Length = 333733

 Score = 26.6 bits (57), Expect = 7.1
 Identities = 25/121 (20%), Positives = 53/121 (43%), Gaps = 24/121 (19%)
 Frame = -1

Query: 20    NLSESTTDQDKLVIARGKLVAPSVVGUSI----KKMPELYNFLMERWALYHNLE------ 69
             N+  +T  Q + ++ R  +    ++  SI    K   EL  FL E+W+   NLE      
Sbjct: 83908 NIGLNTLPQFRKLLFRETIKTSQLIQDSIHDSLKDSSELNIFLKEKWSEVRNLEELNLKW 83729

Query: 70    YDSGEDKNPRLIFYNDKDEEVKVV---------PVKKM-----KADEISSLLDSLGFYKR 115
             +   E +N   + +N+   +V ++         P+ ++     K++E   +  ++G Y +
Sbjct: 83728 HQYAESENWYYVKFNEVLIQVNIINGTLLINHAPIGRLPEIITKSNEYQRIFQNMGKYMK 83549

Query: 116   S 116
             +
Sbjct: 83548 N 83546


>gb|GG738892.1| Naegleria gruberi genomic scaffold NAEGRscaffold_48, whole genome
             shotgun sequence
          Length = 342059

 Score = 26.6 bits (57), Expect = 7.1
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -3

Query: 117   EKGEEVPEEFKHFPLRAPRD 136
             +KG+ +PEEFK+  +  P D
Sbjct: 21510 KKGKNIPEEFKNITVNVPSD 21451


>gb|GG738856.1| Naegleria gruberi genomic scaffold NAEGRscaffold_12, whole genome shotgun
              sequence
          Length = 602266

 Score = 26.6 bits (57), Expect = 7.1
 Identities = 10/36 (27%), Positives = 24/36 (66%)
 Frame = +1

Query: 82     FYNDKDEEVKVVPVKKMKADEISSLLDSLGFYKRSE 117
              F+N K + +K   +++++++E+  L ++L FY  +E
Sbjct: 172603 FFNSKIDNLKTYVLEEIESEELVHLENTLSFYDDTE 172710


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.316    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,921,938
Number of Sequences: 784
Number of extensions: 71785
Number of successful extensions: 324
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 51
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 241
Number of HSP's gapped (non-prelim): 155
length of query: 138
length of database: 13,654,695
effective HSP length: 91
effective length of query: 47
effective length of database: 13,583,351
effective search space: 638417497
effective search space used: 638417497
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)