TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fep15_dre selenoprotein [Danio rerio] (138 letters) Database: E.siliculosus/genome.fa 35 sequences; 195,934,119 total letters Searching...................................done Score E Sequences producing significant alignments: (bits) Value emb|FN649756.1| Ectocarpus siliculosus strain Ec 32, whole genom... 30 1.9 emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genom... 30 2.4 emb|FN649729.1| Ectocarpus siliculosus strain Ec 32, whole genom... 28 7.1 emb|FN649747.1| Ectocarpus siliculosus strain Ec 32, whole genom... 28 9.3 >emb|FN649756.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG31 Length = 577967 Score = 30.4 bits (67), Expect = 1.9 Identities = 14/36 (38%), Positives = 18/36 (50%) Frame = +2 Query: 62 WALYHNLEYDSGEDKNPRLIFYNDKDEEVKVVPVKK 97 WAL +E D D +P L K E + VPVK+ Sbjct: 356285 WALSERVEIDCARDSDPDLRRPRQKHERIPTVPVKR 356392 >emb|FN649727.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG02 Length = 9289656 Score = 30.0 bits (66), Expect = 2.4 Identities = 21/72 (29%), Positives = 33/72 (45%) Frame = +2 Query: 9 LALCATGRTAENLSESTTDQDKLVIARGKLVAPSVVGUSIKKMPELYNFLMERWALYHNL 68 L LC++ AE++ E++ DKL A ++++ VVG P +N L YH Sbjct: 6352283 LPLCSSSAFAEDV-ETSPPPDKLACATSRVISCGVVGRHAHAAPHTWNLLT---VSYHY* 6352450 Query: 69 EYDSGEDKNPRL 80 Y + RL Sbjct: 6352451 NYPFAGNNMQRL 6352486 >emb|FN649729.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG04 Length = 5529539 Score = 28.5 bits (62), Expect = 7.1 Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 5/50 (10%) Frame = -2 Query: 86 KDEE-----VKVVPVKKMKADEISSLLDSLGFYKRSEKGEEVPEEFKHFP 130 KDEE V V+ +K K S L+ + Y + G +PE+ +H P Sbjct: 3872365 KDEETAPPGVSVICTRKKKKTSRSPLVPTPNKYSVTPAGHTIPEQIEHLP 3872216 >emb|FN649747.1| Ectocarpus siliculosus strain Ec 32, whole genome shotgun sequence assembly, chromosome LG22 Length = 2337862 Score = 28.1 bits (61), Expect = 9.3 Identities = 13/29 (44%), Positives = 18/29 (62%) Frame = -2 Query: 97 KMKADEISSLLDSLGFYKRSEKGEEVPEE 125 K++ D IS LDS+GF GE+VP + Sbjct: 101223 KLRVDGISKYLDSVGFRFDHAFGEDVPNQ 101137 Database: E.siliculosus/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 195,934,119 Number of sequences in database: 35 Lambda K H 0.316 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 27,769,732 Number of Sequences: 35 Number of extensions: 286297 Number of successful extensions: 1144 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 1124 Number of HSP's gapped (non-prelim): 75 length of query: 138 length of database: 65,311,373 effective HSP length: 101 effective length of query: 37 effective length of database: 65,307,838 effective search space: 2416390006 effective search space used: 2416390006 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 61 (28.1 bits)