TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SelTryp; hypothetical protein [Trypanosoma brucei TREU927]
PARTIALLY FROM XP_844760.1
         (259 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738864.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    29   3.7  

>gb|GG738864.1| Naegleria gruberi genomic scaffold NAEGRscaffold_20, whole genome shotgun
              sequence
          Length = 441990

 Score = 29.3 bits (64), Expect = 3.7
 Identities = 27/111 (24%), Positives = 56/111 (50%), Gaps = 1/111 (0%)
 Frame = -1

Query: 37     YSSHRYKYLEMLKEAD-LKQMLHEKGEAFHHLRSKRELILAVLKLEKREEALRKARVTDT 95
              +++ +  Y + LK+ + LK++  E+ E+  + R KR  +   +KLE+ E   RK R+TD 
Sbjct: 346578 FTTTKKDYDDFLKKNNPLKELRRERLESETN-RIKR--MGESIKLEENEFQKRKTRITDQ 346408

Query: 96     VQHEVRVEYCSGXGYRRHYEEVAESLLRSLPPELREQQKGKKPFIKFVGVV 146
              + +  +         R   EE++   +  L  +L+E++  +K  +  +  V
Sbjct: 346407 IDYSTKDLRIQEDNLRDQNEEISRE-IAELERKLKEKRAEQKTVLSLLSDV 346258


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.321    0.136    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,807,825
Number of Sequences: 784
Number of extensions: 106384
Number of successful extensions: 431
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 30
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 371
Number of HSP's gapped (non-prelim): 129
length of query: 259
length of database: 13,654,695
effective HSP length: 100
effective length of query: 159
effective length of database: 13,576,295
effective search space: 2158630905
effective search space used: 2158630905
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)