TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SelTryp; hypothetical protein [Trypanosoma brucei TREU927] PARTIALLY FROM XP_844760.1 (259 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668646.1| Blastocystis hominis, Singapore isolate B (sub-t... 37 0.007 emb|FN668647.1| Blastocystis hominis, Singapore isolate B (sub-t... 36 0.019 >emb|FN668646.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_16 Length = 433969 Score = 37.4 bits (85), Expect = 0.007 Identities = 21/63 (33%), Positives = 33/63 (52%), Gaps = 11/63 (17%) Frame = +1 Query: 196 VVGAGFMMNMVASSLLQSGAFEVYLNG-----------SLIYSKLETGAVPTAETLADHI 244 ++G ++N + L +SGAFE+Y NG L+ SKL +G P ET+ + I Sbjct: 235045 IMGVVAVLNYLCEYLQKSGAFEIYFNGIS*IMCV*RLDKLVSSKLASGDYPEEETITNFI 235224 Query: 245 LRQ 247 +Q Sbjct: 235225 DKQ 235233 >emb|FN668647.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_17 Length = 414243 Score = 35.8 bits (81), Expect = 0.019 Identities = 19/74 (25%), Positives = 44/74 (59%) Frame = -3 Query: 185 IAEWIEQHRGMVVGAGFMMNMVASSLLQSGAFEVYLNGSLIYSKLETGAVPTAETLADHI 244 + E ++++R +++ ++ + ++ + +GAFEVY + +L++SKLE PT + ++ H Sbjct: 82501 LLEGLKKNRYLILMFCYLGYYITTNFVFAGAFEVYFDDTLVFSKLEKMRYPTLD-VSIHF 82325 Query: 245 LRQIISGTAAGTRT 258 L + A+ R+ Sbjct: 82324 LSCCLGLEASAERS 82283 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.321 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,462,387 Number of Sequences: 54 Number of extensions: 58767 Number of successful extensions: 267 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 264 Number of HSP's gapped (non-prelim): 8 length of query: 259 length of database: 6,272,415 effective HSP length: 94 effective length of query: 165 effective length of database: 6,267,339 effective search space: 1034110935 effective search space used: 1034110935 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 58 (26.9 bits)