TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SelQ_toxoplasma_gondii_gladyshev # QUERY (66 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-t... 29 0.35 emb|FN668648.1| Blastocystis hominis, Singapore isolate B (sub-t... 27 1.3 emb|FN668691.1| Blastocystis hominis, Singapore isolate B (sub-t... 25 3.9 emb|FN668643.1| Blastocystis hominis, Singapore isolate B (sub-t... 25 6.7 emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-t... 24 8.7 emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-t... 24 8.7 emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-t... 24 8.7 emb|FN668688.1| Blastocystis hominis, Singapore isolate B (sub-t... 24 8.7 >emb|FN668683.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_5 Length = 953317 Score = 28.9 bits (63), Expect = 0.35 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = -3 Query: 29 RRTWQFRGTIPQNPHLAPRFRPNVNDRYQIRRGRG 63 ++T Q + P PH PR R N +R ++RR RG Sbjct: 536354 QQTHQHQRQQPFRPHAQPRRRQNRGNRAEVRRLRG 536250 >emb|FN668648.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_18 Length = 370504 Score = 26.9 bits (58), Expect = 1.3 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +1 Query: 18 ADRERGIQPEHRRTWQFRGTIPQNPHLAPRFRPNVND 54 A R R ++ HRR R + PH+ PRF + +D Sbjct: 137272 ASRLRALRGTHRRAETRRAPQARQPHVLPRFHFSSSD 137382 >emb|FN668691.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_9 Length = 642864 Score = 25.4 bits (54), Expect = 3.9 Identities = 13/34 (38%), Positives = 15/34 (44%) Frame = +3 Query: 29 RRTWQFRGTIPQNPHLAPRFRPNVNDRYQIRRGR 62 RR W R P P PR++P R RR R Sbjct: 523479 RRLWMCRRWKPWKPRRKPRWKPKPRWRSLARRRR 523580 >emb|FN668643.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_13 Length = 486799 Score = 24.6 bits (52), Expect = 6.7 Identities = 11/25 (44%), Positives = 13/25 (52%) Frame = +3 Query: 26 PEHRRTWQFRGTIPQNPHLAPRFRP 50 P+HR R + Q H A RFRP Sbjct: 162195 PDHRAHQTHRQSASQQAHPAQRFRP 162269 >emb|FN668650.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_2 Length = 1458350 Score = 24.3 bits (51), Expect = 8.7 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = -1 Query: 38 IPQNPHLAPRFRPNVNDRYQIRRGR 62 + Q P LAPR RP + Q RGR Sbjct: 1177565 VAQRPALAPRRRPLREEALQPPRGR 1177491 >emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_23 Length = 240939 Score = 24.3 bits (51), Expect = 8.7 Identities = 7/18 (38%), Positives = 12/18 (66%) Frame = +3 Query: 23 GIQPEHRRTWQFRGTIPQ 40 G+ HR W+ +G++PQ Sbjct: 141612 GVHQNHRNYWRSQGSLPQ 141665 >emb|FN668661.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_3 Length = 1354787 Score = 24.3 bits (51), Expect = 8.7 Identities = 15/44 (34%), Positives = 19/44 (43%), Gaps = 4/44 (9%) Frame = +3 Query: 27 EHRRT----WQFRGTIPQNPHLAPRFRPNVNDRYQIRRGRGGXC 66 +HR T Q+R +P R RP N +IRR G C Sbjct: 1028007 DHRHTRRLALQYRSKVPHTSRA--RLRPFANFEKRIRRSHCGDC 1028132 >emb|FN668688.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_6 Length = 900598 Score = 24.3 bits (51), Expect = 8.7 Identities = 9/28 (32%), Positives = 16/28 (57%) Frame = -2 Query: 21 ERGIQPEHRRTWQFRGTIPQNPHLAPRF 48 ERG+ + + W +G P +PH P++ Sbjct: 646734 ERGVCLQRQGVWLCKGGKPISPHY*PKY 646651 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.326 0.143 0.484 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,311,902 Number of Sequences: 54 Number of extensions: 16480 Number of successful extensions: 157 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 144 Number of HSP's gapped (non-prelim): 29 length of query: 66 length of database: 6,272,415 effective HSP length: 41 effective length of query: 25 effective length of database: 6,270,201 effective search space: 156755025 effective search space used: 156755025 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 51 (24.3 bits)