TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SelJ_tetraodon_nigroviridis 
         (189 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    29   2.8  
gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   8.3  

>gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome
             shotgun sequence
          Length = 707703

 Score = 28.9 bits (63), Expect = 2.8
 Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 4/48 (8%)
 Frame = +3

Query: 58    PTALESVIRQLRDPNRKQAQD----LDQAVVGHIHQVKENLSKTPQEL 101
             P   + VI++    N  Q +D    L+ AVV   +Q+KE L+K P+ L
Sbjct: 72237 PIPPDDVIQETSSENSDQDKDMIHTLETAVVNWANQIKEVLNKDPESL 72380


>gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_11, whole genome shotgun
              sequence
          Length = 613270

 Score = 27.3 bits (59), Expect = 8.3
 Identities = 13/33 (39%), Positives = 16/33 (48%), Gaps = 4/33 (12%)
 Frame = -1

Query: 141    GCTCSRGSFIGAC----LGAQVGLEGIPASWSS 169
              GC C  G +IG C    +G  +G   I  SW S
Sbjct: 313990 GCFC*DGVWIGCCDGIVIGVLIGCSSIEVSWLS 313892


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.319    0.132    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,950,859
Number of Sequences: 784
Number of extensions: 88052
Number of successful extensions: 308
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 29
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 252
Number of HSP's gapped (non-prelim): 116
length of query: 189
length of database: 13,654,695
effective HSP length: 96
effective length of query: 93
effective length of database: 13,579,431
effective search space: 1262887083
effective search space used: 1262887083
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)