TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SelJ_tetraodon_nigroviridis (189 letters) Database: B.hominis/genome.fa 54 sequences; 18,817,245 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-t... 28 2.4 emb|FN668691.1| Blastocystis hominis, Singapore isolate B (sub-t... 27 4.0 emb|FN668688.1| Blastocystis hominis, Singapore isolate B (sub-t... 27 5.3 emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-t... 27 6.9 emb|FN668656.1| Blastocystis hominis, Singapore isolate B (sub-t... 27 6.9 emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-t... 26 9.0 >emb|FN668638.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_0 Length = 1966360 Score = 28.1 bits (61), Expect = 2.4 Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 2/46 (4%) Frame = -3 Query: 143 TCSRGSFIGACLGAQVGL--EGIPASWSSRTQRYESVLEHAKKITS 186 TC+ F+GA LG +G G P+S SS + E A TS Sbjct: 387836 TCATAGFLGAALGFSLGFSSSGSPSSSSSSSSESSESSEKANA*TS 387699 Score = 26.6 bits (57), Expect = 6.9 Identities = 8/18 (44%), Positives = 13/18 (72%) Frame = +1 Query: 71 PNRKQAQDLDQAVVGHIH 88 PNR + +DL+Q + H+H Sbjct: 1656994 PNRYKLEDLEQVIAAHVH 1657047 >emb|FN668691.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_9 Length = 642864 Score = 27.3 bits (59), Expect = 4.0 Identities = 13/38 (34%), Positives = 20/38 (52%), Gaps = 8/38 (21%) Frame = +3 Query: 136 TMSCGGCTCSRGSFIGA--------CLGAQVGLEGIPA 165 +++C GC C G IGA C+GA + G+P+ Sbjct: 133653 SIACSGCGCGCGCSIGAIESIDCTGCIGAMGCICGVPS 133766 >emb|FN668688.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_6 Length = 900598 Score = 26.9 bits (58), Expect = 5.3 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +1 Query: 145 SRGSFIGACLGAQVGLEGIPASWSSRTQR 173 SR + G C+G + G + ASW+S R Sbjct: 662326 SRSRWTGVCIGCKTGSKSRTASWTSSKWR 662412 >emb|FN668654.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_23 Length = 240939 Score = 26.6 bits (57), Expect = 6.9 Identities = 14/29 (48%), Positives = 16/29 (55%), Gaps = 5/29 (17%) Frame = -2 Query: 139 CGGCT----CSRGSFIGACLGAQVG-LEG 162 CG C CS+ S G CLG +G LEG Sbjct: 200906 CGNCCDSTGCSQASECGTCLGVGIGCLEG 200820 >emb|FN668656.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_25 Length = 182596 Score = 26.6 bits (57), Expect = 6.9 Identities = 14/34 (41%), Positives = 17/34 (50%), Gaps = 2/34 (5%) Frame = +1 Query: 138 SCGGCTCSRGSFIGACLGAQVGLEG--IPASWSS 169 S GC+CSRG AC + PA+WSS Sbjct: 87352 SSPGCSCSRGRKFSACSSPRRPRRSSPSPATWSS 87453 >emb|FN668639.1| Blastocystis hominis, Singapore isolate B (sub-type 7) whole genome shotgun sequence assembly, scaffold_1 Length = 1866585 Score = 26.2 bits (56), Expect = 9.0 Identities = 11/38 (28%), Positives = 23/38 (60%) Frame = -3 Query: 24 VEDAVRVTQNNDACVAETLAAARFLEHFILNGPDPTAL 61 V+ + +TQ++ ++E + R ++ FI+ G DPT + Sbjct: 152890 VQADITITQSSTGSLSEGGLSRRNMKGFIIQGGDPTGI 152777 Database: B.hominis/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 18,817,245 Number of sequences in database: 54 Lambda K H 0.319 0.132 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,781,834 Number of Sequences: 54 Number of extensions: 52552 Number of successful extensions: 271 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 244 Number of HSP's gapped (non-prelim): 89 length of query: 189 length of database: 6,272,415 effective HSP length: 91 effective length of query: 98 effective length of database: 6,267,501 effective search space: 614215098 effective search space used: 614215098 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 56 (26.2 bits)