TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000114_1.0 # Protein # Selenoprotein N (SelN) # Mus musculus # Complete (557 letters) Database: C.parvum/genome.fa 8 sequences; 9,102,324 total letters Searching........done Score E Sequences producing significant alignments: (bits) Value gb|CM000432.1| Cryptosporidium parvum Iowa II chromosome 4, whol... 28 7.0 gb|CM000429.1| Cryptosporidium parvum Iowa II chromosome 1, whol... 28 7.0 >gb|CM000432.1| Cryptosporidium parvum Iowa II chromosome 4, whole genome shotgun sequence Length = 1104417 Score = 27.7 bits (60), Expect = 7.0 Identities = 12/24 (50%), Positives = 17/24 (70%) Frame = +1 Query: 61 QESALKVLGTDGLFLFSSLDTDQD 84 +ES +K+ G LFLF +DT+QD Sbjct: 302065 KESLIKLSGFTDLFLFIQVDTEQD 302136 Score = 27.7 bits (60), Expect = 7.0 Identities = 14/23 (60%), Positives = 17/23 (73%) Frame = -3 Query: 30 LALLGALLAAAAAVAAARACALL 52 + LL AL AAAAA AAA+A L+ Sbjct: 398092 IVLLAALFAAAAAAAAAKASLLI 398024 Score = 27.3 bits (59), Expect = 9.2 Identities = 16/35 (45%), Positives = 20/35 (57%), Gaps = 2/35 (5%) Frame = +3 Query: 18 AQPAPPRRRARALALLG--ALLAAAAAVAAARACA 50 A P PP++R R + AL AAAAA AA A + Sbjct: 397980 APPEPPKKRGRGRPRINREALAAAAAAAAANNAAS 398084 >gb|CM000429.1| Cryptosporidium parvum Iowa II chromosome 1, whole genome shotgun sequence Length = 875659 Score = 27.7 bits (60), Expect = 7.0 Identities = 10/28 (35%), Positives = 20/28 (71%) Frame = +1 Query: 509 FLDITSMKPEDMENNNVFSFSSSFEDPS 536 FL++ + ++E ++VFS +SF++PS Sbjct: 849712 FLELALLDDSNIEESDVFSIGASFKEPS 849795 Database: C.parvum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 9,102,324 Number of sequences in database: 8 Lambda K H 0.320 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,106,360 Number of Sequences: 8 Number of extensions: 76875 Number of successful extensions: 339 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 309 Number of HSP's gapped (non-prelim): 63 length of query: 557 length of database: 3,034,108 effective HSP length: 97 effective length of query: 460 effective length of database: 3,033,332 effective search space: 1395332720 effective search space used: 1395332720 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 59 (27.3 bits)