TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP12000018_1.0 gi|287325231|ref|NP_001004294.4| selenoprotein N [Danio rerio] (557 letters) Database: P.tricornutum/genome.fa 31 sequences; 23,733,684 total letters Searching...............................done Score E Sequences producing significant alignments: (bits) Value gb|CM000616.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome ... 29 6.2 >gb|CM000616.1| Phaeodactylum tricornutum CCAP 1055/1 chromosome 14, whole genome shotgun sequence Length = 829358 Score = 29.3 bits (64), Expect = 6.2 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = -1 Query: 155 LRSWKRPAISSSTFYASQFKVFLPPSGKSAVGDTWWIIPSEL 196 LR+W+R S T FLPP G+ W +I ++L Sbjct: 91046 LRTWRRSLSSPVTIPPILCSCFLPPCGRRTFTTLWILISTQL 90921 Database: P.tricornutum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 23,733,684 Number of sequences in database: 31 Lambda K H 0.318 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,412,525 Number of Sequences: 31 Number of extensions: 278920 Number of successful extensions: 1317 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 1287 Number of HSP's gapped (non-prelim): 110 length of query: 557 length of database: 7,911,228 effective HSP length: 103 effective length of query: 454 effective length of database: 7,908,035 effective search space: 3590247890 effective search space used: 3590247890 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 62 (28.5 bits)