TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP12000018_1.0 gi|287325231|ref|NP_001004294.4| selenoprotein
N [Danio rerio]
         (557 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    31   2.8  
gb|GG738905.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    31   3.6  
gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    30   4.7  
gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    30   4.7  

>gb|GG738883.1| Naegleria gruberi genomic scaffold NAEGRscaffold_39, whole genome
             shotgun sequence
          Length = 371401

 Score = 31.2 bits (69), Expect = 2.8
 Identities = 25/82 (30%), Positives = 39/82 (47%), Gaps = 3/82 (3%)
 Frame = -2

Query: 35    ISSLFIIAAIPVISFCIKYYLDIQFVKRHEAGLKALGADG---LFFFSSLDTDHDLYLSP 91
             ISS+F+I     +  C  YY  ++ +K   A +K     G   +   SS + + DL   P
Sbjct: 27951 ISSVFLIVYFIYLIGCFIYY-GVKKIKGLTASMKKKEGGGESTIQLESSSNFNFDLEKKP 27775

Query: 92    EEFKPIAEKLTGVAPPPEYEEE 113
             +E K + EK+T      EY+ E
Sbjct: 27774 QEEKTVKEKVTEWIASREYDIE 27709


>gb|GG738905.1| Naegleria gruberi genomic scaffold NAEGRscaffold_61, whole genome shotgun
              sequence
          Length = 290827

 Score = 30.8 bits (68), Expect = 3.6
 Identities = 17/38 (44%), Positives = 22/38 (57%)
 Frame = -3

Query: 84     DHDLYLSPEEFKPIAEKLTGVAPPPEYEEEIPHDPNGE 121
              D D+ + PE+     E+L  +A PPE EEEIP  P  E
Sbjct: 131354 DEDIPIPPED----DEELGDIAIPPEDEEEIPIPPEEE 131253


>gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_14, whole genome shotgun
              sequence
          Length = 782252

 Score = 30.4 bits (67), Expect = 4.7
 Identities = 23/102 (22%), Positives = 46/102 (45%), Gaps = 5/102 (4%)
 Frame = +1

Query: 460    LVKELEDLQGDVKNLELSEKARLHLEKYTFPVQMMVVLPNGTVVHHINANNFL-----DQ 514
              L+ EL  ++G ++N+ + +  +L+LEKY++ V     + + T+       NFL     D 
Sbjct: 162952 LLAELLRMEGKIENVYVLDSYKLYLEKYSYVVTFKDRIGHQTLYPSEIIENFLFLGNRDN 163131

Query: 515    TSMKPEDEGPGLSFSAGFEDPSTSTYIRFLQEGLEKAKPYLE 556
               S   E    G+++     +   +     + +  EK   YL+
Sbjct: 163132 ASSDRELTDIGITYILNMAEEVENVLEEMILDNGEKKYKYLK 163257


>gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome shotgun
              sequence
          Length = 707703

 Score = 30.4 bits (67), Expect = 4.7
 Identities = 20/59 (33%), Positives = 27/59 (45%), Gaps = 3/59 (5%)
 Frame = -2

Query: 52     KYYLDIQFVKRHEAGLKALGADGL---FFFSSLDTDHDLYLSPEEFKPIAEKLTGVAPP 107
              K+  DI  +   E G +    DG    FFF + D    + + PEE K IAE    +A P
Sbjct: 505562 KFDADIYVLTAEEGGRRTAFVDGFSPQFFFRTADVTGKMKIYPEEGKDIAEGEKCMAMP 505386


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.318    0.136    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,787,789
Number of Sequences: 784
Number of extensions: 416552
Number of successful extensions: 1720
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 31
Number of HSP's that attempted gapping in prelim test: 1481
Number of HSP's gapped (non-prelim): 653
length of query: 557
length of database: 13,654,695
effective HSP length: 107
effective length of query: 450
effective length of database: 13,570,807
effective search space: 6106863150
effective search space used: 6106863150
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 64 (29.3 bits)