TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) #
Homo sapiens # Complete
         (197 letters)

Database: P.pallidum/genome.fa 
           42 sequences; 32,942,533 total letters

Searching..........................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GL290996.1| Polysphondylium pallidum PN500 unplaced genomic s...    57   1e-08
gb|GL291006.1| Polysphondylium pallidum PN500 unplaced genomic s...    28   3.3  
gb|GL290990.1| Polysphondylium pallidum PN500 unplaced genomic s...    27   7.3  
gb|GL290986.1| Polysphondylium pallidum PN500 unplaced genomic s...    27   9.6  

>gb|GL290996.1| Polysphondylium pallidum PN500 unplaced genomic scaffold
             PPL_scaffold14, whole genome shotgun sequence
          Length = 1054919

 Score = 56.6 bits (135), Expect = 1e-08
 Identities = 45/161 (27%), Positives = 64/161 (39%), Gaps = 52/161 (32%)
 Frame = +2

Query: 85    DLHARYAECGLRILAFPCNQFGKQEPGSNEEIK------EFAAGYNVKFDMFSKIC---- 134
             DL  ++      IL FPC QF  Q PGS +EI           G+   F MF+K+     
Sbjct: 66080 DLVEKFGTTEFVILGFPCAQFMNQSPGSGDEILLTLKYIRPGNGFQPAFPMFAKVNNNNS 66259

Query: 135   ------------------------VNGDDA--HPLWKWMKIQPKGKG------------- 155
                                     VNGD +  +P++ W+K    G G             
Sbjct: 66260 L*N**NLILMHFQPNFLYDPLQSNVNGDPSTVNPVFNWLK---SGCGPITQTILETSLIS 66430

Query: 156   ---ILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193
                ++ N I WNF KFL+ K G + KRY P   P ++ +D+
Sbjct: 66431 WTPVMTNDITWNFEKFLVSKTGQLYKRYSPQTLPELLAEDI 66553



 Score = 42.4 bits (98), Expect(2) = 2e-08
 Identities = 25/77 (32%), Positives = 39/77 (50%), Gaps = 18/77 (23%)
 Frame = +2

Query: 135   VNGDDAH--PLWKWMKIQPKGKG----------------ILGNAIKWNFTKFLIDKNGCV 176
             VNGD +   P+++W++    G G                +L N I WNF KFL+ K G +
Sbjct: 68381 VNGDPSQISPVFQWLR---SGCGATSQTIIDTSLISWTPVLTNDITWNFEKFLVSKTGQL 68551

Query: 177   VKRYGPMEEPLVIEKDL 193
             V+RY P   P ++ +D+
Sbjct: 68552 VRRYSPETFPQLLAEDI 68602



 Score = 32.7 bits (73), Expect(2) = 2e-08
 Identities = 23/74 (31%), Positives = 37/74 (50%), Gaps = 6/74 (8%)
 Frame = +1

Query: 66    VTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEE----IKEFAA 121
             +  + S+   T +   +LV+   +Y      IL FPC+QF  Q PGS++E    +K    
Sbjct: 68083 ILTLKSKFINTFIGCNELVE---KYGTEEFAILGFPCSQFMNQAPGSDQEFLLTLKYVRP 68253

Query: 122   GYNV--KFDMFSKI 133
             G N    F +F+K+
Sbjct: 68254 GDNFVPNFLLFTKV 68295


>gb|GL291006.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig9,
              whole genome shotgun sequence
          Length = 1029409

 Score = 28.5 bits (62), Expect = 3.3
 Identities = 13/33 (39%), Positives = 18/33 (54%), Gaps = 3/33 (9%)
 Frame = +1

Query: 16     CGALAAPGLAGTMCASRDDWRCARSMH---EFS 45
              C + A PG    +C + D WRC+R  +   EFS
Sbjct: 844363 CSSSAVPGGRVALCDAPDRWRCSRGRYGRAEFS 844461


>gb|GL290990.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold8,
               whole genome shotgun sequence
          Length = 2270872

 Score = 27.3 bits (59), Expect = 7.3
 Identities = 16/51 (31%), Positives = 26/51 (50%)
 Frame = +1

Query: 76      TEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVK 126
               TE+    L ++H   +E  +RILA  CN+       S++ +KE+  G   K
Sbjct: 1844632 TEIRAMHLTNIHVNDSEIMVRILASVCNETSLI---SSDLLKEYLLGSTYK 1844775


>gb|GL290986.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold4,
              whole genome shotgun sequence
          Length = 1229170

 Score = 26.9 bits (58), Expect = 9.6
 Identities = 14/52 (26%), Positives = 25/52 (48%)
 Frame = +3

Query: 79     NYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMF 130
              NYT+ ++ +A +              F  ++ G  +EI +F  GY+  F+MF
Sbjct: 575820 NYTKRIEKYASWKN--------KLQTFDPKKDGDVDEIIKFVVGYDFPFEMF 575951


  Database: P.pallidum/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 32,942,533
  Number of sequences in database:  42
  
Lambda     K      H
   0.324    0.140    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,424,926
Number of Sequences: 42
Number of extensions: 102151
Number of successful extensions: 331
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 313
Number of HSP's gapped (non-prelim): 33
length of query: 197
length of database: 10,980,844
effective HSP length: 95
effective length of query: 102
effective length of database: 10,976,854
effective search space: 1119639108
effective search space used: 1119639108
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)