TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) # Homo sapiens # Complete (197 letters) Database: N.gruberi/genome.fa 784 sequences; 40,964,085 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value gb|GG738848.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 139 1e-33 gb|GG738879.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 137 6e-33 gb|GG738908.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 124 5e-29 gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 116 1e-26 gb|GG738888.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 94 3e-22 gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 86 2e-17 gb|GG738867.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 48 6e-06 gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 36 0.025 gb|GG739086.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 29 3.1 gb|GG739061.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 28 4.0 gb|GG738862.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 28 4.0 gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_... 28 4.0 >gb|GG738848.1| Naegleria gruberi genomic scaffold NAEGRscaffold_4, whole genome shotgun sequence Length = 712659 Score = 139 bits (351), Expect = 1e-33 Identities = 71/154 (46%), Positives = 102/154 (66%), Gaps = 1/154 (0%) Frame = -3 Query: 41 MHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAF 100 ++ FS KD D + V+L +Y+G V ++ NVAS+ G T+ Y L +++ +Y E G +LAF Sbjct: 531028 IYSFSVKDADLNEVSLSEYKGKVLLIVNVASKCGFTK-QYDDLQEVYNKYKEQGFEVLAF 530852 Query: 101 PCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGN 159 PCNQFG QEPG+NEEI FA + + F +F K+ VNG PL+ ++K +G G L + Sbjct: 530851 PCNQFGSQEPGTNEEICTFARSKFKTTFKIFDKVEVNGSGTIPLYAYLK--KEGSGFLVD 530678 Query: 160 AIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193 A+KWNFTKFL+ K+G V+KRY P P +E D+ Sbjct: 530677 AVKWNFTKFLVSKSGKVLKRYSPTTNPKDLEDDI 530576 Score = 40.4 bits (93), Expect = 0.001 Identities = 44/186 (23%), Positives = 76/186 (40%), Gaps = 35/186 (18%) Frame = +2 Query: 36 RCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQU-----GKTEVN----YTQLVDL 86 + A S +E A+DI G + + G VC++ NV K ++ + QL++L Sbjct: 102896 KLANSFYELKAQDIKGKVTEFKSFEGKVCLIVNVDEDELIRMKVKHQITHPEEFKQLMEL 103075 Query: 87 HA---RYAECGLRILAFPCNQFGKQEPG------------SNEEIKEFAAGYNVKFDMFS 131 ++ I+AFP + N++ G +V F + Sbjct: 103076 RELIHKFENKEFSIIAFPTREAEVTHEHVAPEYTLDDVLLDNDKKASKIIGKDV-FTVMR 103252 Query: 132 KICVNGDDAHPLWKWMKIQPKG---------KGILGNAIKWN--FTKFLIDKNGCVVKRY 180 + +N H ++ ++K Q + + + G I N FTKFLI++ G VV R+ Sbjct: 103253 PVHLNTMKEHQVYAYLKYQAEELEKKIVKHPEELKGEDISINEMFTKFLINRKGEVVARF 103432 Query: 181 GPMEEP 186 G EP Sbjct: 103433 GKNVEP 103450 >gb|GG738879.1| Naegleria gruberi genomic scaffold NAEGRscaffold_35, whole genome shotgun sequence Length = 384281 Score = 137 bits (345), Expect = 6e-33 Identities = 70/149 (46%), Positives = 98/149 (65%), Gaps = 1/149 (0%) Frame = +2 Query: 39 RSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRIL 98 +S+++FS ++DG V++ Y G V ++ N AS G T L L+ R+ G +L Sbjct: 140762 KSLYDFSVLNVDGEEVSMKNYSGKVLLIVNTASYCGFTP-QLGSLQRLYERFKSRGFEVL 140938 Query: 99 AFPCNQFGKQEPGSNEEIKEFAA-GYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGIL 157 AFPCNQFG QEPG+ +E+ FAA YNV F +F K+ VNG DA PL+K++K Q KG L Sbjct: 140939 AFPCNQFGAQEPGNKQEVCSFAAEKYNVSFTIFDKVKVNGKDAVPLFKFLKSQ--SKGFL 141112 Query: 158 GNAIKWNFTKFLIDKNGCVVKRYGPMEEP 186 + +KWNFTKFL+D++G VV RY P+ +P Sbjct: 141113 TSDVKWNFTKFLVDRSGKVVGRYSPITDP 141199 >gb|GG738908.1| Naegleria gruberi genomic scaffold NAEGRscaffold_64, whole genome shotgun sequence Length = 259034 Score = 124 bits (311), Expect = 5e-29 Identities = 77/181 (42%), Positives = 98/181 (54%), Gaps = 29/181 (16%) Frame = +2 Query: 42 HEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFP 101 + F D G+ V L Y+G V +V NVAS GKT Y L L+ +Y + GL ILAFP Sbjct: 225359 YSFVVPDAQGNDVKLSDYKGKVVMVVNVASSCGKTP-QYAGLQKLYEKYKDQGLEILAFP 225535 Query: 102 CNQFGKQ----------------------------EPGSNEEIKEFAAG-YNVKFDMFSK 132 CNQF Q E GSNEEI F Y V F MF+K Sbjct: 225536 CNQFAFQVSCHPDHQFNYFNDNYYSDQFNIIIN**ERGSNEEICTFTRDKYKVTFKMFAK 225715 Query: 133 ICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKD 192 VNG D PL++++K +G+G L NAIKWNFTKFL+ K+G V++RY P EP +E+D Sbjct: 225716 TTVNGGDTIPLYQYLK--KEGEGSLFNAIKWNFTKFLVSKSGKVLQRYSPNTEPEDMEED 225889 Query: 193 L 193 + Sbjct: 225890 I 225892 >gb|GG738855.1| Naegleria gruberi genomic scaffold NAEGRscaffold_11, whole genome shotgun sequence Length = 613270 Score = 116 bits (290), Expect = 1e-26 Identities = 73/186 (39%), Positives = 97/186 (52%), Gaps = 34/186 (18%) Frame = -2 Query: 42 HEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFP 101 + F+ D G+ V+L Y+G V +V NVAS G T Y L L+ +Y + G I+AFP Sbjct: 514602 YSFTVPDAQGNEVSLSDYKGKVVMVVNVASSCGLTP-QYEGLQALYDKYKDQGFEIIAFP 514426 Query: 102 CNQFGKQ---------------------------------EPGSNEEIKEFAAG-YNVKF 127 CNQF Q E GSN+EI FA + V F Sbjct: 514425 CNQFAFQVSF**NLNFE*VSNNFYYFYSTN*INLLIYSV*ERGSNDEICAFARNKFKVSF 514246 Query: 128 DMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187 +F+K VNG + PL+K++K +G+G L AIKWNFTKFL+ K G V+KRY P EP Sbjct: 514245 KIFAKTHVNGSETIPLYKYLK--KEGEGKLFKAIKWNFTKFLVSKTGKVLKRYSPNTEPS 514072 Query: 188 VIEKDL 193 IE+D+ Sbjct: 514071 EIEQDI 514054 >gb|GG738888.1| Naegleria gruberi genomic scaffold NAEGRscaffold_44, whole genome shotgun sequence Length = 351708 Score = 94.0 bits (232), Expect(2) = 3e-22 Identities = 54/114 (47%), Positives = 74/114 (64%), Gaps = 3/114 (2%) Frame = -2 Query: 83 LVDLHARYAECG-LRILAFPCNQFGKQEPGSNEEIKEFAAG-YNVKFDMFSKICVNGDDA 140 L +L+ +Y + G ++AFP N F QE GS EEI F + V F + KI VNGD+A Sbjct: 156239 LQELYDKYGKDGKFEVIAFPSNSFN-QEKGSTEEICAFTKDRFKVTFKIMEKIEVNGDNA 156063 Query: 141 HPLWKWMKIQPKGKGILGNA-IKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193 HP+++++K + G G LG+ IKWNFTKFLI+K G V+ RY P +PL I KD+ Sbjct: 156062 HPIYQFLKQEGPG-GFLGSTNIKWNFTKFLINKEGKVIGRYAPTTDPLSIAKDI 155904 Score = 28.5 bits (62), Expect(2) = 3e-22 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = -1 Query: 51 GHMVNLDKYRGFVCIVTNVASQUGKT 76 G +++ + RG VC++ NVAS G T Sbjct: 156408 GETISMSEMRGKVCLIVNVASNCGFT 156331 >gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_9, whole genome shotgun sequence Length = 695587 Score = 85.9 bits (211), Expect = 2e-17 Identities = 64/198 (32%), Positives = 96/198 (48%), Gaps = 46/198 (23%) Frame = +1 Query: 42 HEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHA------------- 88 + F+ KD D + V++ Y+G V ++ NVAS+ G T+ Y + + L + Sbjct: 556099 YSFTVKDADLNDVSMADYKGKVVMIVNVASRCGFTK-QYGRYLLLESVGNANRINRRNSR 556275 Query: 89 --------RYAECGLRILA------FPCNQFGK------------------QEPGSNEEI 116 R+ G+ + + FP N QEPG+NEEI Sbjct: 556276 SL*QV*GSRF*SIGIPMQSIRISSKFPLNFQSSNLNQTIPHH*KLKFHALNQEPGTNEEI 556455 Query: 117 KEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGC 175 FA + V F +F KI VNG + PL+ ++K +G G L +A+KWNFTKFL+ K+G Sbjct: 556456 CTFARTKFKVTFKIFDKINVNGSETIPLYNFLK--KEGAGFLVDAVKWNFTKFLVSKSGK 556629 Query: 176 VVKRYGPMEEPLVIEKDL 193 V+KRY P P +E D+ Sbjct: 556630 VLKRYAPNTSPKDMEDDI 556683 >gb|GG738867.1| Naegleria gruberi genomic scaffold NAEGRscaffold_23, whole genome shotgun sequence Length = 424055 Score = 47.8 bits (112), Expect = 6e-06 Identities = 51/181 (28%), Positives = 75/181 (41%), Gaps = 25/181 (13%) Frame = -1 Query: 38 ARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRI 97 A + + +S KDI G V+L +++G + +V + +L L RYAE G + Sbjct: 132194 AINFYNYSVKDIRGKWVHLREFKGKLSLVAICNTDNPNYYAFLKELQQLKDRYAEKGFEV 132015 Query: 98 LAFPCNQF---------------GKQEPGSNEEI------KEFAAGYNVKFDMFSKICVN 136 L P F + E NE E+ +V +D + Sbjct: 132014 LVIPRYPFKPTSSTATPLRKFLCDEIETSRNELFVCKGVPHEYETCSSVNYDALTVKAEC 131835 Query: 137 GDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYG---PMEEPL-VIEKD 192 GD H L + + K I +F KFLIDKNG VV R G P+E+ + IEK+ Sbjct: 131834 GDAYHYLSNFREGSSK-------TIVEDFEKFLIDKNGAVVYRCGNNTPLEDVVPKIEKE 131676 Query: 193 L 193 L Sbjct: 131675 L 131673 >gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_1, whole genome shotgun sequence Length = 1555553 Score = 35.8 bits (81), Expect = 0.025 Identities = 25/101 (24%), Positives = 46/101 (45%), Gaps = 11/101 (10%) Frame = -2 Query: 48 DIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARY-----------AECGLR 96 ++DG V R + + N Q ++ NY +++ H R A G+ Sbjct: 889687 NLDGDHVFHTLQRNEIVALINSFKQHSQSIYNYHEMITYHNRKQYAPLIAVVYGAIVGIA 889508 Query: 97 ILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNG 137 +L + F KQ+P N+E K+ + +++ SK+C+NG Sbjct: 889507 VLLLGVSFFRKQKPEENKEKKD*SLLFSIFSLYLSKMCLNG 889385 >gb|GG739086.1| Naegleria gruberi genomic scaffold NAEGRscaffold_277, whole genome shotgun sequence Length = 8164 Score = 28.9 bits (63), Expect = 3.1 Identities = 15/52 (28%), Positives = 31/52 (59%) Frame = -3 Query: 53 MVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQ 104 ++ + KY FV + +++S N+ L +LH++++ L +L+FPC+Q Sbjct: 1814 LLEMSKYISFV*FLLDLSS-------NFEFLHNLHSKHSLNLLELLSFPCHQ 1680 >gb|GG739061.1| Naegleria gruberi genomic scaffold NAEGRscaffold_240, whole genome shotgun sequence Length = 8540 Score = 28.5 bits (62), Expect = 4.0 Identities = 15/52 (28%), Positives = 30/52 (57%) Frame = +1 Query: 53 MVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQ 104 ++ + KY FV + +++S N+ L +LH++++ L +L+FPC Q Sbjct: 2839 LLEMSKYISFV*FLLDLSS-------NFEFLHNLHSKHSLNLLELLSFPCRQ 2973 >gb|GG738862.1| Naegleria gruberi genomic scaffold NAEGRscaffold_18, whole genome shotgun sequence Length = 502116 Score = 28.5 bits (62), Expect = 4.0 Identities = 15/52 (28%), Positives = 30/52 (57%) Frame = -1 Query: 53 MVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQ 104 ++ + KY FV + +++S N+ L +LH++++ L +L+FPC Q Sbjct: 349950 LLEMSKYISFV*FLLDLSS-------NFEFLHNLHSKHSLNLLELLSFPCRQ 349816 Score = 28.1 bits (61), Expect = 5.3 Identities = 25/105 (23%), Positives = 42/105 (40%), Gaps = 5/105 (4%) Frame = +1 Query: 34 DW--RCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYA 91 DW + +++F + + ++D Y + + + GK V Y + +L Y Sbjct: 253390 DWGFMSEQQLNKFDLILMSDTIYHVDSYDRLYRAIKSSLKKGGKVYVFYCNINNLL**YR 253569 Query: 92 ECGLRILAFPCNQFGKQEPGSNEEIKEF---AAGYNVKFDMFSKI 133 CG + F C GS ++ KEF G NVK + I Sbjct: 253570 LCGAKSYYFGCG-------GSTKQFKEFIQQKEGSNVKIETVKDI 253683 >gb|GG738850.1| Naegleria gruberi genomic scaffold NAEGRscaffold_6, whole genome shotgun sequence Length = 707703 Score = 28.5 bits (62), Expect = 4.0 Identities = 14/48 (29%), Positives = 24/48 (50%), Gaps = 2/48 (4%) Frame = +3 Query: 44 FSAKDIDGHMV--NLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHAR 89 F AK +GH+ L K CI N+ KT ++ + ++++H R Sbjct: 388275 FVAKPNEGHLAIARLSKLCNLRCITQNIDHLHSKTSLSPSHIIEVHGR 388418 Database: N.gruberi/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 40,964,085 Number of sequences in database: 784 Lambda K H 0.324 0.140 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,975,570 Number of Sequences: 784 Number of extensions: 143142 Number of successful extensions: 513 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 51 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 403 Number of HSP's gapped (non-prelim): 231 length of query: 197 length of database: 13,654,695 effective HSP length: 96 effective length of query: 101 effective length of database: 13,579,431 effective search space: 1371522531 effective search space used: 1371522531 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 59 (27.3 bits)