TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) #
Homo sapiens # Complete
         (197 letters)

Database: C.muris/genome.fa 
           84 sequences; 9,245,251 total letters

Searching...................................................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|DS989726.1| Cryptosporidium muris RN66 scf_1106632373453 geno...    87   3e-18
gb|DS989735.1| Cryptosporidium muris RN66 scf_1106632353989 geno...    27   2.2  
gb|DS989727.1| Cryptosporidium muris RN66 scf_1106632353999 geno...    25   8.4  

>gb|DS989726.1| Cryptosporidium muris RN66 scf_1106632373453 genomic scaffold, whole
              genome shotgun sequence
          Length = 1324930

 Score = 86.7 bits (213), Expect = 3e-18
 Identities = 48/144 (33%), Positives = 85/144 (59%), Gaps = 5/144 (3%)
 Frame = -3

Query: 39     RSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRIL 98
              +S ++++ K ++G +  L   +G V ++TNVAS+ G +   Y Q+V +H+ +A  G  IL
Sbjct: 165521 KSFYDYTLKTLEGELYPLSSLKGKVVMITNVASKCGYSYKYYNQMVRMHSVFAPFGFEIL 165342

Query: 99     AFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMK-----IQPKG 153
              A P  +F +QE    ++I++    +NV+F +     VNG++A  L  ++K     +  + 
Sbjct: 165341 AIPSREFLRQEYLDPKDIRKAINSFNVEFPVMELSNVNGENALDLINFLKFNTPELYDRN 165162

Query: 154    KGILGNAIKWNFTKFLIDKNGCVV 177
              K  L  AI WNF++FL++K+G VV
Sbjct: 165161 KNEL-KAISWNFSRFLVNKSGQVV 165093


>gb|DS989735.1| Cryptosporidium muris RN66 scf_1106632353989 genomic scaffold, whole
             genome shotgun sequence
          Length = 378465

 Score = 27.3 bits (59), Expect = 2.2
 Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
 Frame = +1

Query: 142   PLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVK-RYGPMEEPLVIEKDLP 194
             P W+ ++ Q + +  LG ++ +      I K     K R+     P+ IEKDLP
Sbjct: 49795 PEWRRLRTQAELRNELGLSVPFKSDSIYIQKQDRPAKKRFNETPVPVDIEKDLP 49956


>gb|DS989727.1| Cryptosporidium muris RN66 scf_1106632353999 genomic scaffold, whole
              genome shotgun sequence
          Length = 1306717

 Score = 25.4 bits (54), Expect = 8.4
 Identities = 9/33 (27%), Positives = 20/33 (60%)
 Frame = +3

Query: 153    GKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEE 185
              G G+ G A+ +  ++F   K  C+++R+G + +
Sbjct: 399843 GGGVTGTALFYTLSRFTNLKRICLIERHGDVSQ 399941


  Database: C.muris/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 9,245,251
  Number of sequences in database:  84
  
Lambda     K      H
   0.324    0.140    0.456 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,983,735
Number of Sequences: 84
Number of extensions: 25960
Number of successful extensions: 90
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 75
Number of HSP's gapped (non-prelim): 32
length of query: 197
length of database: 3,081,750
effective HSP length: 87
effective length of query: 110
effective length of database: 3,074,442
effective search space: 338188620
effective search space used: 338188620
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 53 (25.0 bits)