TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000059_1.0 # Protein # Selenoprotein R  (SelR) #
Caenorhabditis elegans # Complete
         (152 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738902.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    83   1e-16
gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    30   0.81 
gb|GG738848.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    30   1.1  
gb|GG738888.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    28   4.0  
gb|GG738901.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    27   9.0  

>gb|GG738902.1| Naegleria gruberi genomic scaffold NAEGRscaffold_58, whole genome shotgun
              sequence
          Length = 305921

 Score = 82.8 bits (203), Expect = 1e-16
 Identities = 51/169 (30%), Positives = 74/169 (43%), Gaps = 47/169 (27%)
 Frame = +3

Query: 29     EWKSVLPNEVYRVARESGTETPHTGGFN--------DHFEKGRYVCLCCGSELFNSDA-- 78
              EW  +L ++ Y V R +GTE     G+         ++ E+G + C  C + LF++    
Sbjct: 188622 EWMKILTSKQYEVLRRAGTEPSFCSGYKVFKDQLKENNQEEGVFHCSGCDAPLFSAKTSF 188801

Query: 79     -------------------------------------KFWAGCGWPAFSESVGQDANIVR 101
                                                   +F+ G GWP+F       A I  
Sbjct: 188802 DSGKCFINNSV*FISYINDSIICQFEFIGMHSSIIWNEFYKGSGWPSFWAPFKPKA-IGY 188978

Query: 102    IVDRSHGMHRTEVRCKTCDAHLGHVFNDGPKETTGERYXINSVCMAFEK 150
              +VD+  GM R EV C  C +HLGHVF DGP++ T  RY IN+VC+A +K
Sbjct: 188979 VVDKKFGMERVEVVCTNCHSHLGHVFEDGPRDKTKLRYCINAVCLALKK 189125


>gb|GG738853.1| Naegleria gruberi genomic scaffold NAEGRscaffold_9, whole genome shotgun
              sequence
          Length = 695587

 Score = 30.0 bits (66), Expect = 0.81
 Identities = 12/36 (33%), Positives = 17/36 (47%)
 Frame = +3

Query: 44     ESGTETPHTGGFNDHFEKGRYVCLCCGSELFNSDAK 79
              ES       G  N + E+  + C+CCG  + N D K
Sbjct: 194670 ESNIPAQEKGDANKYCEQVNFKCICCGKAIQNGDQK 194777


>gb|GG738848.1| Naegleria gruberi genomic scaffold NAEGRscaffold_4, whole genome shotgun
              sequence
          Length = 712659

 Score = 29.6 bits (65), Expect = 1.1
 Identities = 15/60 (25%), Positives = 25/60 (41%), Gaps = 1/60 (1%)
 Frame = -3

Query: 27     QTEWKSVLPNEVYRVARESGTETPHTGGFNDHFEKGRYVC-LCCGSELFNSDAKFWAGCG 85
              + EW+    N + +   E   E        D++EK  Y+C LC  + L  +    +  CG
Sbjct: 230662 ENEWREEEENHLKQFEEEEALEAEDLQQLIDYYEKSVYLCPLCKKNHLLETKHSLFCKCG 230483



 Score = 28.1 bits (61), Expect = 3.1
 Identities = 11/20 (55%), Positives = 12/20 (60%)
 Frame = +1

Query: 57     DHFEKGRYVCLCCGSELFNS 76
              D F +G Y CLCC   LF S
Sbjct: 516073 DRFTRGNYCCLCCLR*LFQS 516132


>gb|GG738888.1| Naegleria gruberi genomic scaffold NAEGRscaffold_44, whole genome shotgun
              sequence
          Length = 351708

 Score = 27.7 bits (60), Expect = 4.0
 Identities = 13/35 (37%), Positives = 21/35 (60%)
 Frame = +1

Query: 47     TETPHTGGFNDHFEKGRYVCLCCGSELFNSDAKFW 81
              TE  H  G++D   KG ++C  C S+L +++ K W
Sbjct: 214780 TEPHHVRGYHDVR*KGFFLCEICASDL-STNQKHW 214881


>gb|GG738901.1| Naegleria gruberi genomic scaffold NAEGRscaffold_57, whole genome shotgun
              sequence
          Length = 309005

 Score = 26.6 bits (57), Expect = 9.0
 Identities = 13/33 (39%), Positives = 24/33 (72%), Gaps = 1/33 (3%)
 Frame = +3

Query: 14     SKLKVEKNPKDVKQTEWKSVLPNE-VYRVARES 45
              S+L+ EK+PK + QT  +S LPN  + ++A+++
Sbjct: 267648 SELQHEKSPKSLLQTTIQSQLPNSTILKIAQKN 267746


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.318    0.134    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,358,107
Number of Sequences: 784
Number of extensions: 101231
Number of successful extensions: 469
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 48
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 366
Number of HSP's gapped (non-prelim): 187
length of query: 152
length of database: 13,654,695
effective HSP length: 93
effective length of query: 59
effective length of database: 13,581,783
effective search space: 801325197
effective search space used: 801325197
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)