TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000020_1.0 # Protein # Selenoprotein P (SelP) # Homo
sapiens # Complete
         (381 letters)

Database: N.gruberi/genome.fa 
           784 sequences; 40,964,085 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    39   0.006
gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_...    30   2.9  

>gb|GG738858.1| Naegleria gruberi genomic scaffold NAEGRscaffold_14, whole genome shotgun
              sequence
          Length = 782252
 
 Score = 39.3 bits (90), Expect = 0.006
 Identities = 27/92 (29%), Positives = 43/92 (46%)
 Frame = +2

Query: 63     ILQASKLEDLRVKLKKEGYSNISYIVVNHQGISSRLKYTHLKNKVSEHIPVYQQEENQTD 122
              ++Q  ++ED+  K+ K+   N S +    Q        T  +N    H    QQ+ENQT 
Sbjct: 268289 VMQFLEMEDVLSKMDKQIEFNHSTLPQQQQ---QTFNNTSSQNNSHRHSKTVQQQENQTT 268459

Query: 123    VWTLLNGSKDDFLIYDRCGRLVYHLGLPFSFL 154
              +  +  G K +F+I  RCG++        SFL
Sbjct: 268460 I-PIQKGRKKEFVISTRCGQIQLTFERVLSFL 268552



>gb|GG738845.1| Naegleria gruberi genomic scaffold NAEGRscaffold_1, whole genome shotgun
              sequence
          Length = 1555553

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 25/85 (29%), Positives = 38/85 (44%)
 Frame = -1

Query: 36     IRDQDPMLNSNGSVTVVALLQASXYLCILQASKLEDLRVKLKKEGYSNISYIVVNHQGIS 95
              IR++   L SN       L QA   L  +Q  K E +++ L +   SN   I   H  + 
Sbjct: 844871 IREEKISLESN-------LAQARYQLVSVQNDK-ESMKLSLLQRIESNEETIKALHDQLQ 844716

Query: 96     SRLKYTHLKNKVSEHIPVYQQEENQ 120
              S L+Y H  N+  +     Q E+N+
Sbjct: 844715 SNLQYVHSSNESVKKTQDEQNEQNK 844641


  Database: N.gruberi/genome.fa
    Posted date:  Feb 9, 2011  9:02 AM
  Number of letters in database: 40,964,085
  Number of sequences in database:  784
  
Lambda     K      H
   0.315    0.130    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,868,751
Number of Sequences: 784
Number of extensions: 229036
Number of successful extensions: 1065
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 22
Number of HSP's that attempted gapping in prelim test: 880
Number of HSP's gapped (non-prelim): 373
length of query: 381
length of database: 13,654,695
effective HSP length: 104
effective length of query: 277
effective length of database: 13,573,159
effective search space: 3759765043
effective search space used: 3759765043
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)