TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|302129654|ref|NP_001180474.1| selenoprotein U [Oryzias latipes] (212 letters) Database: C.owczarzaki/genome.fa 89 sequences; 28,043,798 total letters Searching........................................................................................done Score E Sequences producing significant alignments: (bits) Value supercontig_1.4 of Capsaspora owczarzaki ATCC 30864 67 1e-11 supercontig_1.1 of Capsaspora owczarzaki ATCC 30864 28 5.5 >supercontig_1.4 of Capsaspora owczarzaki ATCC 30864 Length = 2141673 Score = 66.6 bits (161), Expect = 1e-11 Identities = 50/190 (26%), Positives = 85/190 (44%), Gaps = 10/190 (5%) Frame = -3 Query: 31 ANASLEFLEEADLRCTLDHTKVIKAKSLWDKNGAVVMAVRRPGUFLCREEASELSSLKPQ 90 A+ +++ LE AD K + +W +++ +RR G LC E+AS + +KPQ Sbjct: 1144336 ASNNIKVLELAD-------KKELVLADMWKDQRVLLILLRRFGCSLCHEQASHVLEIKPQ 1144178 Query: 91 LEELGVPLVAV------VKENLGSEIQDFRPHFAGDIYIDEEKRFYGPL-QRRMGGLGFI 143 L+ GV +V V E + F ++YID E+ Y +R+G L F+ Sbjct: 1144177 LDAAGVKIVLVGTGNRYFAEKFIENVPGNGQRFPAEVYIDPEQTAYKARGLQRVGLLHFL 1143998 Query: 144 RIGVWQNFIRAWKSGYQGNMNGEGFILGGVYVIGAGEQGII---LEHREKQFGDKVDTAD 200 + +A K+ ++ G+G GG+Y++G G I + G VD Sbjct: 1143997 SWTAISEWRKANKNHPNADLQGDGLQTGGIYLVGPGADSAIHFAFNEYDHPVGTLVDNDQ 1143818 Query: 201 VLKAIQKIVP 210 +L A++ P Sbjct: 1143817 ILAAVKATQP 1143788 >supercontig_1.1 of Capsaspora owczarzaki ATCC 30864 Length = 3794667 Score = 27.7 bits (60), Expect = 5.5 Identities = 26/102 (25%), Positives = 43/102 (42%), Gaps = 2/102 (1%) Frame = +2 Query: 94 LGVPLVAVVKENLGSEIQDFRPHFAGDIYIDEEKRFYGPLQRRMGGLGFIRIGVWQNFIR 153 LG +VAVV+ + ++ R H G+ F G R GGL R + R Sbjct: 1529093 LGAAIVAVVQSRM--MVRGSRRHGRGE--------FRGTRHAREGGLATQRRAAVRRVRR 1529242 Query: 154 AWKSGYQGNMNGEGFILGGVYVIGA--GEQGIILEHREKQFG 193 W G++ G +LG V+ + Q ++++ +Q G Sbjct: 1529243 CWVVEVGGSLLGNFELLGKVFGLHCHLAMQNLLVDFTIRQLG 1529368 Database: C.owczarzaki/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 28,043,798 Number of sequences in database: 89 Lambda K H 0.320 0.140 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,575,071 Number of Sequences: 89 Number of extensions: 92232 Number of successful extensions: 344 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 1 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 330 Number of HSP's gapped (non-prelim): 58 length of query: 212 length of database: 9,347,932 effective HSP length: 95 effective length of query: 117 effective length of database: 9,339,477 effective search space: 1092718809 effective search space used: 1092718809 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 58 (26.9 bits)