TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= scaffold_323 [translate(1)] (147 letters) Database: P.pallidum/genome.fa 42 sequences; 32,942,533 total letters Searching..........................................done Score E Sequences producing significant alignments: (bits) Value gb|GL290990.1| Polysphondylium pallidum PN500 unplaced genomic s... 98 2e-23 gb|GL290995.1| Polysphondylium pallidum PN500 unplaced genomic s... 28 3.1 gb|GL290985.1| Polysphondylium pallidum PN500 unplaced genomic s... 27 4.0 gb|GL290998.1| Polysphondylium pallidum PN500 unplaced genomic s... 27 5.3 gb|GL290984.1| Polysphondylium pallidum PN500 unplaced genomic s... 27 5.3 gb|GL291010.1| Polysphondylium pallidum PN500 unplaced genomic s... 27 6.9 >gb|GL290990.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold8, whole genome shotgun sequence Length = 2270872 Score = 97.8 bits (242), Expect(2) = 2e-23 Identities = 61/159 (38%), Positives = 80/159 (50%), Gaps = 57/159 (35%) Frame = -2 Query: 28 CHELGFTDSLLCSTCKDFAEFVGDSD--LNNECAKCCAEESENIK--------------- 70 CH+LGFTDSLLCS+CKD FVGD++ + EC KCC+ E+ K Sbjct: 2086860 CHDLGFTDSLLCSSCKDLEGFVGDANSAVIEECKKCCSLEATQQKVY*TKLNYKSNLNFI 2086681 Query: 71 ----------------KTYLSATLIACKXRLGAFPHIKDFIDNKAKDYNK------FK-- 106 KT++SA L C+ +LGAFP +KDFIDNK+K++NK FK Sbjct: 2086680 LIISNKLIIN**Q***KTFVSAQLTICR*KLGAFPSVKDFIDNKSKNFNKVSVNVSFKLI 2086501 Query: 107 ----------------FTHEQGANPRLVLRDTEGKEEEI 129 F + GA+P + L DTEGK E+I Sbjct: 2086500 IINVMYIILILLLLLYF*YVSGASPVIELTDTEGKVEKI 2086384 Score = 27.3 bits (59), Expect(2) = 2e-23 Identities = 11/14 (78%), Positives = 12/14 (85%) Frame = -1 Query: 130 SIDGWKSENLEEFL 143 SID WK EN+EEFL Sbjct: 2086303 SIDKWKVENIEEFL 2086262 Score = 26.6 bits (57), Expect = 6.9 Identities = 13/35 (37%), Positives = 17/35 (48%), Gaps = 2/35 (5%) Frame = -2 Query: 49 VGDSDLNNEC--AKCCAEESENIKKTYLSATLIAC 81 VGD+ + +C CC ENI YL +I C Sbjct: 1980771 VGDTAIIGDCNCCSCCLYSFENINSAYL*NGIIIC 1980667 >gb|GL290995.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold13, whole genome shotgun sequence Length = 1052162 Score = 27.7 bits (60), Expect = 3.1 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = -2 Query: 94 FIDNKAKDYNKFKFTHEQGANPRLVLRDT 122 F++NK N F F H+ +NPR + T Sbjct: 359542 FLNNKNNSNNSFTFLHKHLSNPRTINNQT 359456 >gb|GL290985.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold3, whole genome shotgun sequence Length = 2290287 Score = 27.3 bits (59), Expect = 4.0 Identities = 24/91 (26%), Positives = 37/91 (40%), Gaps = 1/91 (1%) Frame = -3 Query: 39 CSTCKDFAEFVGDSDLNNECAKCCAEESENIKKTYLSATLIACKXRLGAFPHIKDFIDNK 98 CSTC +F + C+KCC +E T L+ T + A P +D + Sbjct: 492094 CSTCTNF----------DVCSKCCNKEPIVKTTTALATTTTSTTTAAAAEPPKRDGL--- 491954 Query: 99 AKDYNKFKFTH-EQGANPRLVLRDTEGKEEE 128 F+FT Q +LV++ K+ E Sbjct: 491953 ------FRFTSLSQKQQEKLVVQPAHDKQNE 491879 Score = 26.9 bits (58), Expect = 5.3 Identities = 10/34 (29%), Positives = 19/34 (55%) Frame = -3 Query: 31 LGFTDSLLCSTCKDFAEFVGDSDLNNECAKCCAE 64 +G+ ++ S CKD +E V ++N+C + E Sbjct: 255724 VGYDGAMSISACKDMSETVAKCLIDNQCTRTLPE 255623 >gb|GL290998.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig1, whole genome shotgun sequence Length = 1301413 Score = 26.9 bits (58), Expect = 5.3 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = +2 Query: 106 KFTHEQGANPRLVLRDTEGKEEEISIDGW 134 +F H++ PR LR KE+ IS DGW Sbjct: 1218689 EFRHDESQLPRCNLRVWLHKEQ*ISSDGW 1218775 >gb|GL290984.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_scaffold2, whole genome shotgun sequence Length = 3242543 Score = 26.9 bits (58), Expect = 5.3 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = -3 Query: 41 TCKDFAEFVGDSDLNNECAKCCAEESENI 69 T ++ EFVG ++C +CC +E+ NI Sbjct: 19761 TWENHFEFVG*KRCFSDCGRCCIKENLNI 19675 >gb|GL291010.1| Polysphondylium pallidum PN500 unplaced genomic scaffold PPL_contig13, whole genome shotgun sequence Length = 836226 Score = 26.6 bits (57), Expect = 6.9 Identities = 9/27 (33%), Positives = 18/27 (66%) Frame = -2 Query: 53 DLNNECAKCCAEESENIKKTYLSATLI 79 D +++ CCA+++ NI TY+ ++I Sbjct: 475475 DNSSKIGSCCADDNGNIYVTYIGTSII 475395 Database: P.pallidum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 32,942,533 Number of sequences in database: 42 Lambda K H 0.324 0.140 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,409,571 Number of Sequences: 42 Number of extensions: 68684 Number of successful extensions: 349 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 314 Number of HSP's gapped (non-prelim): 96 length of query: 147 length of database: 10,980,844 effective HSP length: 91 effective length of query: 56 effective length of database: 10,977,022 effective search space: 614713232 effective search space used: 614713232 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 56 (26.2 bits)