TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= scaffold_323 [translate(1)] (147 letters) Database: C.parvum/genome.fa 8 sequences; 9,102,324 total letters Searching........done Score E Sequences producing significant alignments: (bits) Value gb|CM000431.1| Cryptosporidium parvum Iowa II chromosome 3, whol... 27 1.3 gb|CM000435.1| Cryptosporidium parvum Iowa II chromosome 7, whol... 26 3.7 gb|CM000436.1| Cryptosporidium parvum Iowa II chromosome 8, whol... 25 8.3 >gb|CM000431.1| Cryptosporidium parvum Iowa II chromosome 3, whole genome shotgun sequence Length = 1099352 Score = 27.3 bits (59), Expect = 1.3 Identities = 21/52 (40%), Positives = 28/52 (53%) Frame = -2 Query: 96 DNKAKDYNKFKFTHEQGANPRLVLRDTEGKEEEISIDGWKSENLEEFLIQNN 147 D + K +K E G+ P L + DT +E ISI+G K ENLE QN+ Sbjct: 524287 DKEEKFDSKVGEPTEGGSTPVLAI-DTSNLDE-ISIEGIKLENLEVSQTQND 524138 >gb|CM000435.1| Cryptosporidium parvum Iowa II chromosome 7, whole genome shotgun sequence Length = 1278458 Score = 25.8 bits (55), Expect = 3.7 Identities = 17/45 (37%), Positives = 20/45 (44%), Gaps = 4/45 (8%) Frame = -1 Query: 58 CAKCCAEESENIKKTYLSATLIACKXR----LGAFPHIKDFIDNK 98 C C E E KK Y CK R L F H++D +DNK Sbjct: 1118285 CPLCMEEMDETDKKFY------PCKCRYQICLWCFYHVRDQLDNK 1118169 Score = 25.8 bits (55), Expect = 3.7 Identities = 9/17 (52%), Positives = 13/17 (76%) Frame = +3 Query: 43 KDFAEFVGDSDLNNECA 59 KD+ E V +DLNN+C+ Sbjct: 1120044 KDYEELVSKNDLNNKCS 1120094 >gb|CM000436.1| Cryptosporidium parvum Iowa II chromosome 8, whole genome shotgun sequence Length = 1344712 Score = 24.6 bits (52), Expect = 8.3 Identities = 16/62 (25%), Positives = 28/62 (45%), Gaps = 6/62 (9%) Frame = +3 Query: 91 IKDFIDNKAKDYNKFKFTHEQGAN------PRLVLRDTEGKEEEISIDGWKSENLEEFLI 144 +KD +D + Y FKF H Q N + + + + K +I D ++L E + Sbjct: 75333 LKD-LDERIAKYELFKFNHSQRLNSVDLKISKDIQKKDDNKRRKIDNDHLLDQSLTEIDL 75509 Query: 145 QN 146 +N Sbjct: 75510 KN 75515 Database: C.parvum/genome.fa Posted date: Feb 9, 2011 9:02 AM Number of letters in database: 9,102,324 Number of sequences in database: 8 Lambda K H 0.324 0.140 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,411,031 Number of Sequences: 8 Number of extensions: 18173 Number of successful extensions: 105 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 84 Number of HSP's gapped (non-prelim): 48 length of query: 147 length of database: 3,034,108 effective HSP length: 83 effective length of query: 64 effective length of database: 3,033,444 effective search space: 194140416 effective search space used: 194140416 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 51 (24.3 bits)