BLASTP 2.2.25+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Stephen F. Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. RID: RF8GZY2F016 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 13,331,964 sequences; 4,567,125,576 total letters Query= Length=49 Score E Sequences producing significant alignments: (Bits) Value ref|XP_627815.1| transporter protein with conserved Zn ribbon... 99.4 1e-19 ref|XP_666698.1| transporter [Cryptosporidium hominis TU502] ... 99.4 2e-19 ref|XP_002142720.1| hypothetical protein [Cryptosporidium mur... 80.9 5e-14 ref|XP_642710.1| hypothetical protein DDB_G0277321 [Dictyoste... 46.6 0.001 emb|CBZ55290.1| conserved hypothetical protein [Neospora cani... 46.2 0.001 ref|XP_642959.1| hypothetical protein DDB_G0276943 [Dictyoste... 45.8 0.002 emb|CBN76966.1| conserved unknown protein [Ectocarpus silicul... 44.7 0.004 gb|EGC39087.1| hypothetical protein DICPUDRAFT_148215 [Dictyo... 42.7 0.015 ref|XP_002364603.1| hypothetical protein, conserved [Toxoplas... 42.7 0.017 gb|EEE25916.1| conserved hypothetical protein [Toxoplasma gon... 42.7 0.017 gb|EGC39283.1| hypothetical protein DICPUDRAFT_52756 [Dictyos... 41.2 0.044 gb|EGB06974.1| hypothetical protein AURANDRAFT_65191 [Aureoco... 37.7 0.47 ref|XP_646615.1| hypothetical protein DDB_G0270204 [Dictyoste... 37.4 0.75 gb|EFA83226.1| putative transmembrane protein [Polysphondyliu... 35.0 3.5 gb|EFW46169.1| predicted protein [Capsaspora owczarzaki ATCC ... 34.7 3.8 ref|NP_939795.1| hypothetical protein DIP1447 [Corynebacteriu... 33.5 8.8 ALIGNMENTS >ref|XP_627815.1| transporter protein with conserved Zn ribbon C11C7CxxC [Cryptosporidium parvum Iowa II] emb|CAD98306.1| putative transporter [Cryptosporidium parvum] gb|EAK90068.1| transporter protein with conserved Zn ribbon C11C7CxxC [Cryptosporidium parvum Iowa II] Length=446 Score = 99.4 bits (246), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 49/49 (100%), Positives = 49/49 (100%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF Sbjct 304 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 352 >ref|XP_666698.1| transporter [Cryptosporidium hominis TU502] gb|EAL36465.1| transporter [Cryptosporidium hominis] Length=446 Score = 99.4 bits (246), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 49/49 (100%), Positives = 49/49 (100%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF Sbjct 304 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 352 >ref|XP_002142720.1| hypothetical protein [Cryptosporidium muris RN66] gb|EEA08371.1| hypothetical protein, conserved [Cryptosporidium muris RN66] Length=446 Score = 80.9 bits (198), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 35/49 (72%), Positives = 44/49 (90%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C Q PVLIYFIAN+ICN+FS++V+KHG+A+TGFIVST+RLP+TT VF Sbjct 310 CELVQLPVLIYFIANIICNIFSIMVIKHGSAATGFIVSTMRLPLTTFVF 358 >ref|XP_642710.1| hypothetical protein DDB_G0277321 [Dictyostelium discoideum AX4] sp|Q550A6.1|CRTP2_DICDI RecName: Full=Crt homolog 2; AltName: Full=Chloroquine resistance transporter paralog 2; Short=DdCRTp2 gb|EAL68847.1| hypothetical protein DDB_G0277321 [Dictyostelium discoideum AX4] Length=484 Score = 46.6 bits (109), Expect = 0.001, Method: Compositional matrix adjust. Identities = 25/49 (52%), Positives = 32/49 (66%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C+ A VLIY NV+ NVF +LVLKH A+ I +TLRLP+T + F Sbjct 324 CHGAWVVVLIYMAVNVLYNVFILLVLKHAGATVFSIANTLRLPLTNIAF 372 >emb|CBZ55290.1| conserved hypothetical protein [Neospora caninum Liverpool] Length=874 Score = 46.2 bits (108), Expect = 0.001, Method: Composition-based stats. Identities = 23/49 (47%), Positives = 32/49 (66%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C A V +Y N++ N+F + V+K+G A+ F+VSTLRLPVT L F Sbjct 583 CEGAWKYVGMYLGFNLLYNMFIIFVVKNGGAALTFLVSTLRLPVTALAF 631 >ref|XP_642959.1| hypothetical protein DDB_G0276943 [Dictyostelium discoideum AX4] sp|Q9GSB0.1|CRTP1_DICDI RecName: Full=Crt homolog 1; AltName: Full=Chloroquine resistance transporter paralog 1; Short=DdCRTp1 gb|AAG31811.1|AF317500_1 putative transporter protein SSA662 [Dictyostelium discoideum] gb|EAL68973.1| hypothetical protein DDB_G0276943 [Dictyostelium discoideum AX4] Length=473 Score = 45.8 bits (107), Expect = 0.002, Method: Compositional matrix adjust. Identities = 22/49 (45%), Positives = 34/49 (70%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C++A +++Y N+I N+F +LVLKH A+ I +TLRLP+T +VF Sbjct 318 CHNAWIVIILYMTINIIYNIFILLVLKHAGATVYSIANTLRLPLTNIVF 366 >emb|CBN76966.1| conserved unknown protein [Ectocarpus siliculosus] Length=561 Score = 44.7 bits (104), Expect = 0.004, Method: Composition-based stats. Identities = 18/49 (37%), Positives = 32/49 (66%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C +A F V +Y N++ NV +++LKHG+A+ F+ ST+ +P+ + F Sbjct 272 CETAPFFVTLYLCFNLVYNVVLIVILKHGSANVLFLASTVMVPIGNVAF 320 >gb|EGC39087.1| hypothetical protein DICPUDRAFT_148215 [Dictyostelium purpureum] Length=217 Score = 42.7 bits (99), Expect = 0.015, Method: Compositional matrix adjust. Identities = 22/49 (45%), Positives = 33/49 (68%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C++A +L Y NV+ N+F +LV+KH A+ I +TLRLP+T +VF Sbjct 64 CHNAYIIILCYMTINVVYNIFILLVIKHAGATVFSIANTLRLPLTNIVF 112 >ref|XP_002364603.1| hypothetical protein, conserved [Toxoplasma gondii ME49] gb|EEA97462.1| hypothetical protein, conserved [Toxoplasma gondii ME49] gb|EEE33082.1| conserved hypothetical protein [Toxoplasma gondii VEG] Length=512 Score = 42.7 bits (99), Expect = 0.017, Method: Compositional matrix adjust. Identities = 23/49 (47%), Positives = 31/49 (64%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C A V +Y N++ N+F + V+K G A+ F+VSTLRLPVT L F Sbjct 231 CEGAWKYVGMYLSFNLLYNMFIIFVVKSGGAALTFLVSTLRLPVTALAF 279 >gb|EEE25916.1| conserved hypothetical protein [Toxoplasma gondii GT1] Length=476 Score = 42.7 bits (99), Expect = 0.017, Method: Compositional matrix adjust. Identities = 23/49 (47%), Positives = 31/49 (64%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C A V +Y N++ N+F + V+K G A+ F+VSTLRLPVT L F Sbjct 195 CEGAWKYVGMYLSFNLLYNMFIIFVVKSGGAALTFLVSTLRLPVTALAF 243 >gb|EGC39283.1| hypothetical protein DICPUDRAFT_52756 [Dictyostelium purpureum] Length=488 Score = 41.2 bits (95), Expect = 0.044, Method: Compositional matrix adjust. Identities = 21/49 (43%), Positives = 32/49 (66%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C+ A +++Y N+I NV +LV+KH A+ I +TLRLP+T +VF Sbjct 327 CHHAYILIILYMSINIIYNVLILLVIKHAGATVFSIANTLRLPLTNIVF 375 >gb|EGB06974.1| hypothetical protein AURANDRAFT_65191 [Aureococcus anophagefferens] Length=590 Score = 37.7 bits (86), Expect = 0.47, Method: Composition-based stats. Identities = 17/49 (35%), Positives = 32/49 (66%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C SA V +Y + N+ NV +V++LK G+++ ++ ST+ +PV+ +F Sbjct 340 CGSAPLFVGVYVLFNIAYNVLTVVILKKGSSNLLYLGSTILVPVSNAMF 388 >ref|XP_646615.1| hypothetical protein DDB_G0270204 [Dictyostelium discoideum AX4] sp|Q55C66.1|CRTP3_DICDI RecName: Full=Crt homolog 3; AltName: Full=Chloroquine resistance transporter paralog 3; Short=DdCRTp3 gb|EAL72452.1| hypothetical protein DDB_G0270204 [Dictyostelium discoideum AX4] Length=478 Score = 37.4 bits (85), Expect = 0.75, Method: Compositional matrix adjust. Identities = 20/49 (41%), Positives = 30/49 (62%), Gaps = 0/49 (0%) Query 1 CNSAQFPVLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 C++ VLIY N+ N+F +LVLKH A+ I +T+ LP+T + F Sbjct 318 CHNTWIIVLIYMTVNIAYNIFILLVLKHAGATVYSIANTVILPLTNIFF 366 >gb|EFA83226.1| putative transmembrane protein [Polysphondylium pallidum PN500] Length=453 Score = 35.0 bits (79), Expect = 3.5, Method: Compositional matrix adjust. Identities = 17/33 (52%), Positives = 24/33 (73%), Gaps = 0/33 (0%) Query 17 ICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 + NVF +LV+KH A+ I +TLRLP+T +VF Sbjct 326 VYNVFILLVIKHAGATVYSIANTLRLPLTNIVF 358 >gb|EFW46169.1| predicted protein [Capsaspora owczarzaki ATCC 30864] Length=590 Score = 34.7 bits (78), Expect = 3.8, Method: Compositional matrix adjust. Identities = 17/42 (41%), Positives = 29/42 (70%), Gaps = 0/42 (0%) Query 8 VLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRLPVTTLVF 49 +L+Y AN+I NV V+V+K+G+A+ ++ ST LP+ + F Sbjct 440 MLMYLSANLIYNVVLVMVIKYGSAALMYVASTAVLPLGAICF 481 >ref|NP_939795.1| hypothetical protein DIP1447 [Corynebacterium diphtheriae NCTC 13129] emb|CAE49975.1| Putative membrane protein [Corynebacterium diphtheriae] Length=212 Score = 33.5 bits (75), Expect = 8.8, Method: Composition-based stats. Identities = 15/35 (43%), Positives = 24/35 (69%), Gaps = 0/35 (0%) Query 8 VLIYFIANVICNVFSVLVLKHGTASTGFIVSTLRL 42 VLI FIA ++CN+F +L + A+ GF++S + L Sbjct 77 VLIGFIAAIVCNLFLILTRRTVAANIGFLLSGIAL 111 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Mar 8, 2011 4:39 PM Number of letters in database: 272,158,280 Number of sequences in database: 13,331,964 Lambda K H 0.334 0.143 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 13331964 Number of Hits to DB: 33763988 Number of extensions: 733911 Number of successful extensions: 3129 Number of sequences better than 100: 7 Number of HSP's better than 100 without gapping: 0 Number of HSP's gapped: 3129 Number of HSP's successfully gapped: 7 Length of query: 49 Length of database: 4567125576 Length adjustment: 22 Effective length of query: 27 Effective length of database: 4273822368 Effective search space: 115393203936 Effective search space used: 115393203936 T: 11 A: 40 X1: 15 (7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (19.6 bits) S2: 67 (30.4 bits)