TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TR1 #anophagefferens # scaffold_11:subseq(783554,7436).pep  
         (488 letters)

Database: gondiidb.fa 
           3590 sequences; 66,210,174 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|158533829|gb|AAQM02000139.1| Toxoplasma gondii GT1 gcontig_11...   102   2e-21
gi|158533932|gb|AAQM02000036.1| Toxoplasma gondii GT1 gcontig_11...    62   2e-09
gi|158533683|gb|AAQM02000285.1| Toxoplasma gondii GT1 gcontig_11...    60   6e-09
gi|158533926|gb|AAQM02000042.1| Toxoplasma gondii GT1 gcontig_11...    55   2e-07
gi|158533810|gb|AAQM02000158.1| Toxoplasma gondii GT1 gcontig_11...    47   9e-05
gi|158533905|gb|AAQM02000063.1| Toxoplasma gondii GT1 gcontig_11...    32   2.2  
gi|158533917|gb|AAQM02000051.1| Toxoplasma gondii GT1 gcontig_11...    32   2.9  
gi|158533953|gb|AAQM02000015.1| Toxoplasma gondii GT1 gcontig_11...    30   6.4  
gi|158533873|gb|AAQM02000095.1| Toxoplasma gondii GT1 gcontig_11...    30   8.3  

>gi|158533829|gb|AAQM02000139.1| Toxoplasma gondii GT1
             gcontig_1107000936588, whole genome shotgun sequence
          Length = 174004

 Score =  102 bits (253), Expect = 2e-21
 Identities = 47/83 (56%), Positives = 52/83 (62%)
 Frame = -1

Query: 8     VVGGGSGGLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLMHTASII 67
             V+GGGSGGLAC+K  A  GAK  V DFV+PS  GSTWGLGGTCVNVGC+PK L H   + 
Sbjct: 13975 VIGGGSGGLACAKMAAAQGAKTVVFDFVQPSTQGSTWGLGGTCVNVGCVPKYLFHHTGLA 13796

Query: 68    GETLKDDAAAFGWKDAAAGAHDW 90
             G     D    GWK       DW
Sbjct: 13795 GANAHWDGPHMGWKGKFEEQVDW 13727



 Score = 59.7 bits (143), Expect = 1e-08
 Identities = 38/82 (46%), Positives = 49/82 (59%), Gaps = 5/82 (6%)
 Frame = -2

Query: 208   TCMVRSILLRGFDRECVGKIEAHIKHHGVQLQVGVTPAKIEKDAATGVLTVTDSGGG--- 264
             T  VRSILLRGFDR+C  ++   ++  GV++     PAK+ K  A G + VT   G    
Sbjct: 11694 TVAVRSILLRGFDRQCAEQVGLCLEEAGVRILRETIPAKMVKQ-ANGKIQVTFQVGKEKK 11518

Query: 265   --VHEYDTVLVATGRYADTAAL 284
               V E+DTVL ATGR ADT+ L
Sbjct: 11517 ELVEEFDTVLYATGRKADTSNL 11452



 Score = 56.6 bits (135), Expect = 8e-08
 Identities = 33/84 (39%), Positives = 50/84 (59%), Gaps = 12/84 (14%)
 Frame = -3

Query: 378  KAQLGECNVESYISEFAPLEYAL------SETRSERGD-----GCFAKLVVDKSTG-KVV 425
            +A+ G  ++E Y+ +F+PL ++        + R    D      C AKL+  KS   KVV
Sbjct: 9083 QAEFGRDDLEVYLFQFSPLFFSCVHREKAPQARKSPEDVDITPPCLAKLICVKSEDEKVV 8904

Query: 426  GFHYLGPNAGEITQGFSIAMRKGA 449
            G H++GPNAGE+ QGF++A+R GA
Sbjct: 8903 GIHFVGPNAGELMQGFALAVRLGA 8832



 Score = 45.8 bits (107), Expect = 1e-04
 Identities = 23/37 (62%), Positives = 26/37 (70%), Gaps = 1/37 (2%)
 Frame = -2

Query: 453  DFISTVGIHPTVAEEFTSMTVTKSSGES-AAKGGCXG 488
            DF   VGIHPT AE F ++TVTK+SGE   A GGC G
Sbjct: 8181 DFDKCVGIHPTNAEAFMALTVTKASGEPFVASGGCGG 8071



 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 19/39 (48%), Positives = 28/39 (71%)
 Frame = -1

Query: 97    VQDHIKSLNFKYRVALREAGITYLNRLGEFEDKNTISLT 135
             VQ++IKSLNF YR  LR+AG+TY+N   +F   + ++ T
Sbjct: 13294 VQNYIKSLNFGYRTGLRKAGVTYINAYAKFVSPHELAYT 13178



 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 25/41 (60%), Positives = 27/41 (65%)
 Frame = -1

Query: 310   LPGAPHVYAIGDVVEGRPELTPVAIEAGLRLARRLFGGKTE 350
             LPG    +  GD VE  PELTPVAI+AG  LARRLF   TE
Sbjct: 10117 LPG--FAFFSGDAVENFPELTPVAIKAGEILARRLFANSTE 10001



 Score = 34.7 bits (78), Expect = 0.34
 Identities = 18/47 (38%), Positives = 26/47 (55%), Gaps = 4/47 (8%)
 Frame = -1

Query: 338  LRLARRLFGGKTE----PMDYECVATTIFTPLEYGTIGLSEDDAKAQ 380
            LRL    F  + E     MD+  + TT+FTP+EY   G SE+ A+ +
Sbjct: 9628 LRL*NERFNSRNENCVFQMDFTNIPTTVFTPIEYAHTGYSEEAAEVR 9488



 Score = 33.1 bits (74), Expect = 0.98
 Identities = 20/40 (50%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
 Frame = -3

Query: 146   AARFIVAVGGRPNKLGCPGG--EHAIDSDDLFSLPKAPGR 183
             A   +VAVGGRP+      G  E AI SDD+FSL +AP +
Sbjct: 12575 ARNIVVAVGGRPHIPEEVEGAKELAITSDDIFSLKQAPNK 12456



 Score = 32.3 bits (72), Expect = 1.7
 Identities = 12/21 (57%), Positives = 15/21 (71%)
 Frame = -1

Query: 185   LCVGGGYIALECGGFTAGMGY 205
             LCVG  YI+LEC GF   +G+
Sbjct: 12013 LCVGASYISLECAGFLRELGF 11951


>gi|158533932|gb|AAQM02000036.1| Toxoplasma gondii GT1
             gcontig_1107000942138, whole genome shotgun sequence
          Length = 407974

 Score = 62.0 bits (149), Expect = 2e-09
 Identities = 27/57 (47%), Positives = 40/57 (70%)
 Frame = +1

Query: 4     YDVVVVGGGSGGLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKL 60
             +D+VV+GGGSGG+AC++  A  GA VA+++            +GGTCVN+GC+PKK+
Sbjct: 54931 FDLVVIGGGSGGVACARRAASYGASVAIVEKA---------NIGGTCVNLGCMPKKV 55074



 Score = 58.5 bits (140), Expect = 2e-08
 Identities = 32/88 (36%), Positives = 46/88 (52%), Gaps = 1/88 (1%)
 Frame = +3

Query: 154   GGRPNKLGCPGGEHAIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYPTTCMVRS 213
             G R   L  PG E AI SD  F +   P RV  +G GY++ E GG    +G   +  +RS
Sbjct: 56646 GTRRQVLDIPGAEFAISSDGFFQIQHLPRRVALIGAGYVSAELGGILRHLGVDVSIFMRS 56825

Query: 214   -ILLRGFDRECVGKIEAHIKHHGVQLQV 240
                L+ FD+E V  +EA  +  G+Q+ +
Sbjct: 56826 QRQLKRFDKEAVESLEATQRASGIQVSL 56909



 Score = 53.1 bits (126), Expect = 9e-07
 Identities = 27/59 (45%), Positives = 39/59 (66%), Gaps = 1/59 (1%)
 Frame = +2

Query: 329   LTPVAIEAGLRLARRLFGGKTEP-MDYECVATTIFTPLEYGTIGLSEDDAKAQLGECNV 386
             L PVA+ AG  LA RLFGG++E  +D   V T +F+    G +G++E+DAK+  GE N+
Sbjct: 58235 LAPVAVAAGRLLADRLFGGRSEARLDLSVVPTVVFSHPALGAVGMTEEDAKSLYGEENI 58411



 Score = 34.7 bits (78), Expect = 0.34
 Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
 Frame = +3

Query: 375   DDAKAQLGECNVESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTG-KVVGFHYLGPN 433
             D  K +     ++ Y S F    YA            F K+V  K+   KV+G H +G N
Sbjct: 58770 DGVKRECTLVLLQVYTSTFIDSFYAAWSMPPSAKPKSFVKMVCLKTANDKVLGLHLVGRN 58949

Query: 434   AGEITQGFSIAMR 446
               E+ QGF++A++
Sbjct: 58950 VDEMLQGFAVAIK 58988



 Score = 34.7 bits (78), Expect = 0.34
 Identities = 16/28 (57%), Positives = 20/28 (71%)
 Frame = +1

Query: 444   AMRKGATYADFISTVGIHPTVAEEFTSM 471
             A  +GAT ADF ST+ IHPT AEE  ++
Sbjct: 59326 ASYQGATKADFNSTLAIHPTAAEEVVTL 59409


>gi|158533683|gb|AAQM02000285.1| Toxoplasma gondii GT1
             gcontig_1107000860480, whole genome shotgun sequence
          Length = 28843

 Score = 60.5 bits (145), Expect = 6e-09
 Identities = 36/94 (38%), Positives = 50/94 (53%), Gaps = 1/94 (1%)
 Frame = -3

Query: 146   AARFIVAVGGRPNKLGCPGGEHAIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGY 205
             + +F    GGRP K G  G EH I+SD  F L + P +V  +G GYIA+E  G  A M  
Sbjct: 17309 SGKFSPFSGGRPAKAGIEGEEHTINSDGFFELEEMPQKVALLGAGYIAVEFAGVFAAMKC 17130

Query: 206   PTTCMVRS-ILLRGFDRECVGKIEAHIKHHGVQL 238
              T   VR+   LR FD     +++  ++  GVQ+
Sbjct: 17129 ETHLFVRNERALRKFDDMISMRVDEFMRKAGVQV 17028



 Score = 58.5 bits (140), Expect = 2e-08
 Identities = 27/57 (47%), Positives = 37/57 (64%)
 Frame = -2

Query: 4     YDVVVVGGGSGGLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKL 60
             +D+ V+GGGSGGLAC++  A    +V + D  +         LGGTCVNVGC+PKK+
Sbjct: 19206 FDLFVIGGGSGGLACARRAATYNVRVGLADGNR---------LGGTCVNVGCVPKKV 19063



 Score = 53.9 bits (128), Expect = 5e-07
 Identities = 30/61 (49%), Positives = 39/61 (63%), Gaps = 1/61 (1%)
 Frame = -1

Query: 328   ELTPVAIEAGLRLARRLFGGK-TEPMDYECVATTIFTPLEYGTIGLSEDDAKAQLGECNV 386
             ELTPVAI AG RLA RLFGG     +D  CV T +F+      +GL+E +AKA  GE ++
Sbjct: 15631 ELTPVAIAAGRRLADRLFGGLCNAKLDSACVPTVVFSHPPAACVGLTEAEAKATYGEKDI 15452

Query: 387   E 387
             +
Sbjct: 15451 K 15449



 Score = 35.4 bits (80), Expect = 0.20
 Identities = 27/77 (35%), Positives = 35/77 (45%), Gaps = 5/77 (6%)
 Frame = -1

Query: 386   VESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTG-KVVGFHYLGPNAGEITQGFSIA 444
             ++ Y      L Y       E     F K++  KS   KVVG H +G  A E+ QGF +A
Sbjct: 14263 LQVYTGTSVNLYYGAWPVAPEEKPKTFIKMICVKSQMLKVVGLHVVGMGADEMIQGFGVA 14084

Query: 445   MR----KGATYADFIST 457
             M+    K AT   F ST
Sbjct: 14083 MKMVGTKFATTLPFGST 14033



 Score = 31.6 bits (70), Expect = 2.9
 Identities = 13/25 (52%), Positives = 18/25 (72%)
 Frame = -2

Query: 447   KGATYADFISTVGIHPTVAEEFTSM 471
             +GAT ADF + V +HPT AEE  ++
Sbjct: 13485 QGATKADFDNCVAVHPTAAEEVVTL 13411


>gi|158533926|gb|AAQM02000042.1| Toxoplasma gondii GT1
              gcontig_1107000930636, whole genome shotgun sequence
          Length = 384686

 Score = 55.1 bits (131), Expect = 2e-07
 Identities = 29/59 (49%), Positives = 35/59 (59%)
 Frame = +3

Query: 1      GFDYDVVVVGGGSGGLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKK 59
              G  YDVVVVGGG GG   + + A+LG K A ++            LGGTC+NVGCIP K
Sbjct: 233820 GGKYDVVVVGGGPGGYVAAIKAAQLGLKTACVE--------KRGTLGGTCLNVGCIPSK 233972



 Score = 30.0 bits (66), Expect = 8.3
 Identities = 19/50 (38%), Positives = 24/50 (48%), Gaps = 5/50 (10%)
 Frame = -1

Query: 34     FVKPSPAGSTWGLGGTCV-----NVGCIPKKLMHTASIIGETLKDDAAAF 78
              +++ SP   T  L G CV     N+G     LMH  S   E  +DD AAF
Sbjct: 297155 WIRGSPLSQTCRLLGKCVFAFRRNLGRARLLLMHVRSQTAENREDDTAAF 297006


>gi|158533810|gb|AAQM02000158.1| Toxoplasma gondii GT1
             gcontig_1107000948812, whole genome shotgun sequence
          Length = 142546

 Score = 46.6 bits (109), Expect = 9e-05
 Identities = 25/62 (40%), Positives = 33/62 (53%)
 Frame = -2

Query: 4     YDVVVVGGGSGGLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLMHT 63
             +DV ++G G GG A +   A LG K AV+    P         GGTCVN GC+P K +  
Sbjct: 43122 FDVTIIGLGVGGHAAALHAAALGLKTAVVSGGDP---------GGTCVNRGCVPSKALLA 42970

Query: 64    AS 65
             A+
Sbjct: 42969 AA 42964



 Score = 40.8 bits (94), Expect = 0.005
 Identities = 27/95 (28%), Positives = 47/95 (49%), Gaps = 3/95 (3%)
 Frame = -1

Query: 375   DDAKAQLGECN---VESYISEFAPLEYALSETRSERGDGCFAKLVVDKSTGKVVGFHYLG 431
             ++A  +LG  +   V   +S F     A++E   E       K++  K TGK++G H +G
Sbjct: 40216 EEAAMELGAKDGFEVGKSVSHFRANTKAIAEGEGEG----ILKVLYRKDTGKILGCHMIG 40049

Query: 432   PNAGEITQGFSIAMRKGATYADFISTVGIHPTVAE 466
              +A ++ Q  + A+    +  D   TV  HPT++E
Sbjct: 40048 IHASDLIQECATAITNDISVKDLAFTVHTHPTLSE 39944



 Score = 33.9 bits (76), Expect = 0.58
 Identities = 36/125 (28%), Positives = 50/125 (40%), Gaps = 14/125 (11%)
 Frame = -1

Query: 171   SDDLFSLPKAPGRVLCVGGGYIALECGGFTAGMGYPTTCM-VRSILLRGFDRECVGKIE- 228
             SD   SLP  P  +  VG GYI LE       +G     +     LL G D+E     E 
Sbjct: 41803 SDTCVSLPWLPSEICIVGSGYIGLEFMDVFTSLGSEVVMVEAGPRLLPGVDKEVAKLAER 41624

Query: 229   ---AHIKHHGVQLQVGVTPAKIE----KDAATGVLTVTD-----SGGGVHEYDTVLVATG 276
                   K   V+L      +++     K  A   + +TD     S G ++  D  L+ATG
Sbjct: 41623 LLLQQFKERPVKLYTNTLASQVRPLGPKGEAPVEVQLTDAQTKESKGKIYP-DACLIATG 41447

Query: 277   RYADT 281
             R  +T
Sbjct: 41446 RRPNT 41432


>gi|158533905|gb|AAQM02000063.1| Toxoplasma gondii GT1
             gcontig_1107000978084, whole genome shotgun sequence
          Length = 319274

 Score = 32.0 bits (71), Expect = 2.2
 Identities = 21/80 (26%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
 Frame = +3

Query: 4     YDVVVVGGG-SGGLACSKECAKLGAKVAVLDFVKPSPAGSTWGLGGTCVNVGCIPKKLMH 62
             YD VV+G G +G +   +  +   +  ++     P  AGST G+    +  G +P   + 
Sbjct: 74028 YDFVVIGSGTAGSVIAGRLSSSFISPTSLSSSTAPLAAGSTSGVSVLLIEAGNLPDLRIF 74207

Query: 63    TASIIG-ETLKDDAAAFGWK 81
             +  II   TL++  +   WK
Sbjct: 74208 SPHIIPLMTLENQRSGIDWK 74267


>gi|158533917|gb|AAQM02000051.1| Toxoplasma gondii GT1
              gcontig_1107000852440, whole genome shotgun sequence
          Length = 354343

 Score = 31.6 bits (70), Expect = 2.9
 Identities = 17/35 (48%), Positives = 24/35 (68%)
 Frame = -1

Query: 101    IKSLNFKYRVALREAGITYLNRLGEFEDKNTISLT 135
              IKSL++K R A RE G ++L  L + ED+ T +LT
Sbjct: 301606 IKSLSWKSRDASREVGDSFLQLLRKEEDRTTWALT 301502


>gi|158533953|gb|AAQM02000015.1| Toxoplasma gondii GT1
             gcontig_1107000869154, whole genome shotgun sequence
          Length = 552318

 Score = 30.4 bits (67), Expect = 6.4
 Identities = 21/46 (45%), Positives = 23/46 (50%)
 Frame = +2

Query: 156   RPNKLGCPGGEHAIDSDDLFSLPKAPGRVLCVGGGYIALECGGFTA 201
             R NKLG P G HA  +    SLP   G  L VG     L  GGF+A
Sbjct: 11030 RKNKLGAPPGHHA-KALACRSLPSLRGHNLLVGYTSQGLADGGFSA 11164


>gi|158533873|gb|AAQM02000095.1| Toxoplasma gondii GT1
              gcontig_1107000925756, whole genome shotgun sequence
          Length = 243989

 Score = 30.0 bits (66), Expect = 8.3
 Identities = 24/71 (33%), Positives = 30/71 (42%), Gaps = 5/71 (7%)
 Frame = +3

Query: 142    TSVTAARFIVAVGGRPNKLGCP-----GGEHAIDSDDLFSLPKAPGRVLCVGGGYIALEC 196
              T +  + F+V V GR  +         G EH    D L S      RVLC+G  + A   
Sbjct: 115488 TELHISTFLVGVCGRHEQWALA*YCTRGNEHWWSVDRLSSSSSTC*RVLCLGNAWTA--- 115658

Query: 197    GGFTAGMGYPT 207
              G F  G  YPT
Sbjct: 115659 GLFQTGSKYPT 115691


  Database: gondiidb.fa
    Posted date:  Mar 10, 2010  5:54 PM
  Number of letters in database: 66,210,174
  Number of sequences in database:  3590
  
Lambda     K      H
   0.317    0.136    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,615,176
Number of Sequences: 3590
Number of extensions: 629057
Number of successful extensions: 2837
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 247
Number of HSP's successfully gapped in prelim test: 82
Number of HSP's that attempted gapping in prelim test: 1902
Number of HSP's gapped (non-prelim): 1716
length of query: 488
length of database: 22,070,058
effective HSP length: 109
effective length of query: 379
effective length of database: 21,678,748
effective search space: 8216245492
effective search space used: 8216245492
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 65 (29.6 bits)
