TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TR1
         (499 letters)

Database: genome.fa 
           3590 sequences; 66,210,174 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAQM02000139.1| Toxoplasma gondii GT1 gcontig_1107000936588, ...    69   2e-11
gb|AAQM02000036.1| Toxoplasma gondii GT1 gcontig_1107000942138, ...    59   2e-08
gb|AAQM02000285.1| Toxoplasma gondii GT1 gcontig_1107000860480, ...    53   1e-06
gb|AAQM02000158.1| Toxoplasma gondii GT1 gcontig_1107000948812, ...    39   0.024
gb|AAQM02000202.1| Toxoplasma gondii GT1 gcontig_1107000988482, ...    34   0.45 
gb|AAQM02000042.1| Toxoplasma gondii GT1 gcontig_1107000930636, ...    33   1.3  
gb|AAQM02000054.1| Toxoplasma gondii GT1 gcontig_1107000855630, ...    30   8.6  
gb|AAQM02000024.1| Toxoplasma gondii GT1 gcontig_1107000833414, ...    30   8.6  

>gb|AAQM02000139.1| Toxoplasma gondii GT1 gcontig_1107000936588, whole genome shotgun
             sequence
          Length = 174004

 Score = 68.6 bits (166), Expect = 2e-11
 Identities = 33/69 (47%), Positives = 41/69 (59%), Gaps = 1/69 (1%)
 Frame = -1

Query: 39    MVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLG-QALQDSRNYGWKVEETVKHD 97
             +V DFV P+  G+ WGLGGTCVNVGC+PK L H   L G  A  D  + GWK +   + D
Sbjct: 13909 VVFDFVQPSTQGSTWGLGGTCVNVGCVPKYLFHHTGLAGANAHWDGPHMGWKGKFEEQVD 13730

Query: 98    WDRMIEAVQ 106
             W   +E  Q
Sbjct: 13729 WGVCVEVRQ 13703



 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 34/92 (36%), Positives = 56/92 (60%)
 Frame = -2

Query: 209   LAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLR 268
             L+   L VTV VRSILLRGFD+  A ++G  +EE G++ +R+ +P K+ +      G+++
Sbjct: 11718 LSSFSLQVTVAVRSILLRGFDRQCAEQVGLCLEEAGVRILRETIPAKMVK---QANGKIQ 11548

Query: 269   VVAQSTNSEEIIEGEYNTVMLAIGRDACTRKI 300
             V  Q    ++ +  E++TV+ A GR A T  +
Sbjct: 11547 VTFQVGKEKKELVEEFDTVLYATGRKADTSNL 11452



 Score = 62.0 bits (149), Expect = 2e-09
 Identities = 34/89 (38%), Positives = 49/89 (55%), Gaps = 14/89 (15%)
 Frame = -3

Query: 393  KFGEENIEVYHSYFWPLEWTIPSRDNNK--------------CYAKIICNTKDNERVVGF 438
            +FG +++EVY   F PL ++   R+                 C AK+IC   ++E+VVG 
Sbjct: 9077 EFGRDDLEVYLFQFSPLFFSCVHREKAPQARKSPEDVDITPPCLAKLICVKSEDEKVVGI 8898

Query: 439  HVLGPNAGEVTQGFAAALKCGLTKKQLDS 467
            H +GPNAGE+ QGFA A++ G  K  L S
Sbjct: 8897 HFVGPNAGELMQGFALAVRLGAKKVSLRS 8811



 Score = 48.5 bits (114), Expect = 2e-05
 Identities = 21/41 (51%), Positives = 28/41 (68%)
 Frame = -1

Query: 103   EAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKAT 143
             + VQN+I SLN+GYR  LR+  V Y NAY +F+ PH +  T
Sbjct: 13300 QKVQNYIKSLNFGYRTGLRKAGVTYINAYAKFVSPHELAYT 13178



 Score = 40.8 bits (94), Expect = 0.005
 Identities = 19/35 (54%), Positives = 27/35 (77%)
 Frame = -1

Query: 328   YIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGST 362
             + +  GD +E+  ELTPVAI+AG +LA+RL+A ST
Sbjct: 10108 FAFFSGDAVENFPELTPVAIKAGEILARRLFANST 10004



 Score = 39.3 bits (90), Expect = 0.014
 Identities = 17/25 (68%), Positives = 21/25 (84%)
 Frame = -1

Query: 194   LVVGASYVALECAGFLAGIGLDVTV 218
             L VGASY++LECAGFL  +G DV+V
Sbjct: 12013 LCVGASYISLECAGFLRELGFDVSV 11939



 Score = 38.9 bits (89), Expect = 0.018
 Identities = 16/25 (64%), Positives = 19/25 (76%)
 Frame = -1

Query: 366  DYENVPTTVFTPLEYGACGLSEEKA 390
            D+ N+PTTVFTP+EY   G SEE A
Sbjct: 9571 DFTNIPTTVFTPIEYAHTGYSEEAA 9497



 Score = 38.1 bits (87), Expect = 0.031
 Identities = 20/47 (42%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
 Frame = -3

Query: 148   KEKIYSAERFLIATGERPRYLG-IPGDKEYCISSDDLFSLPYCPGKT 193
             ++KI  A   ++A G RP     + G KE  I+SDD+FSL   P KT
Sbjct: 12593 QDKICKARNIVVAVGGRPHIPEEVEGAKELAITSDDIFSLKQAPNKT 12453



 Score = 36.6 bits (83), Expect = 0.091
 Identities = 17/39 (43%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
 Frame = -2

Query: 462  KKQLDSTIGIHPVCAEVFTTLSVTKRSGAS-ILQAGCUG 499
            ++  D  +GIHP  AE F  L+VTK SG   +   GC G
Sbjct: 8187 QRDFDKCVGIHPTNAEAFMALTVTKASGEPFVASGGCGG 8071



 Score = 31.2 bits (69), Expect = 3.8
 Identities = 15/39 (38%), Positives = 21/39 (53%)
 Frame = -1

Query: 296   CTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGD 334
             C     L+  GV+  E TGKI    +  T+ P +YAIG+
Sbjct: 10966 CVSNADLQAAGVETTE-TGKIVCDGDSHTSAPSVYAIGE 10853


>gb|AAQM02000036.1| Toxoplasma gondii GT1 gcontig_1107000942138, whole genome shotgun
             sequence
          Length = 407974

 Score = 58.5 bits (140), Expect = 2e-08
 Identities = 32/61 (52%), Positives = 39/61 (63%), Gaps = 1/61 (1%)
 Frame = +2

Query: 342   LTPVAIQAGRLLAQRLYAG-STVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIE 400
             L PVA+ AGRLLA RL+ G S  + D   VPT VF+    GA G++EE A   +GEENI 
Sbjct: 58235 LAPVAVAAGRLLADRLFGGRSEARLDLSVVPTVVFSHPALGAVGMTEEDAKSLYGEENIN 58414

Query: 401   V 401
             V
Sbjct: 58415 V 58417



 Score = 54.7 bits (130), Expect = 3e-07
 Identities = 30/86 (34%), Positives = 48/86 (55%), Gaps = 1/86 (1%)
 Frame = +3

Query: 162   GERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVR 221
             G R + L IPG  E+ ISSD  F + + P +  ++GA YV+ E  G L  +G+DV++ +R
Sbjct: 56646 GTRRQVLDIPG-AEFAISSDGFFQIQHLPRRVALIGAGYVSAELGGILRHLGVDVSIFMR 56822

Query: 222   S-ILLRGFDQDMANKIGEHMEEHGIK 246
             S   L+ FD++    +       GI+
Sbjct: 56823 SQRQLKRFDKEAVESLEATQRASGIQ 56900



 Score = 47.8 bits (112), Expect = 4e-05
 Identities = 22/62 (35%), Positives = 37/62 (59%), Gaps = 3/62 (4%)
 Frame = +3

Query: 399   IEVYHSYF---WPLEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAA 455
             ++VY S F   +   W++P     K + K++C    N++V+G H++G N  E+ QGFA A
Sbjct: 58803 LQVYTSTFIDSFYAAWSMPPSAKPKSFVKMVCLKTANDKVLGLHLVGRNVDEMLQGFAVA 58982

Query: 456   LK 457
             +K
Sbjct: 58983 IK 58988



 Score = 35.0 bits (79), Expect = 0.27
 Identities = 18/54 (33%), Positives = 31/54 (57%), Gaps = 1/54 (1%)
 Frame = +2

Query: 284   YNTVMLAIGRDACTRKIGLETVGVKINEKTGK-IPVTDEEQTNVPYIYAIGDIL 336
             ++ V++A+        +GLE  GV I+   G  I V   + T++P IYA+GD++
Sbjct: 57626 FDHVIMAVNPAPAIEDLGLEEAGVDIDVNNGGFIKVDAFQNTSIPGIYAVGDVV 57787



 Score = 32.7 bits (73), Expect = 1.3
 Identities = 11/15 (73%), Positives = 15/15 (100%)
 Frame = +1

Query: 55    LGGTCVNVGCIPKKL 69
             +GGTCVN+GC+PKK+
Sbjct: 55030 IGGTCVNLGCMPKKV 55074


>gb|AAQM02000285.1| Toxoplasma gondii GT1 gcontig_1107000860480, whole genome shotgun
             sequence
          Length = 28843

 Score = 52.8 bits (125), Expect = 1e-06
 Identities = 30/64 (46%), Positives = 40/64 (62%), Gaps = 1/64 (1%)
 Frame = -1

Query: 339   KVELTPVAIQAGRLLAQRLYAG-STVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEE 397
             ++ELTPVAI AGR LA RL+ G    K D   VPT VF+       GL+E +A   +GE+
Sbjct: 15637 QIELTPVAIAAGRRLADRLFGGLCNAKLDSACVPTVVFSHPPAACVGLTEAEAKATYGEK 15458

Query: 398   NIEV 401
             +I+V
Sbjct: 15457 DIKV 15446



 Score = 42.4 bits (98), Expect = 0.002
 Identities = 31/103 (30%), Positives = 57/103 (55%), Gaps = 5/103 (4%)
 Frame = -3

Query: 267   LRVVAQSTNSEEIIEGE----YNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDE- 321
             +R  A  + + E+  GE    +++V++++GR      +GL+ VGVK  ++ G   V DE 
Sbjct: 16427 VRQEADKSLTLELTNGESFRGFDSVIVSVGRVPEVANLGLDVVGVK--QRHGGYIVADEF 16254

Query: 322   EQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVK 364
             + T+V  IYA+GD+   KV     + + G++    L AG+ ++
Sbjct: 16253 QNTSVEQIYAVGDV-SGKVSWRVGSREKGKIQGAALSAGNVLR 16128



 Score = 42.4 bits (98), Expect = 0.002
 Identities = 20/52 (38%), Positives = 28/52 (53%)
 Frame = -1

Query: 411   WTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTK 462
             W +   +  K + K+IC      +VVG HV+G  A E+ QGF  A+K   TK
Sbjct: 14218 WPVAPEEKPKTFIKMICVKSQMLKVVGLHVVGMGADEMIQGFGVAMKMVGTK 14063



 Score = 35.4 bits (80), Expect = 0.20
 Identities = 13/15 (86%), Positives = 15/15 (100%)
 Frame = -2

Query: 55    LGGTCVNVGCIPKKL 69
             LGGTCVNVGC+PKK+
Sbjct: 19107 LGGTCVNVGCVPKKV 19063


>gb|AAQM02000158.1| Toxoplasma gondii GT1 gcontig_1107000948812, whole genome shotgun
             sequence
          Length = 142546

 Score = 38.5 bits (88), Expect = 0.024
 Identities = 31/107 (28%), Positives = 54/107 (50%), Gaps = 7/107 (6%)
 Frame = -1

Query: 178   ISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRS--ILLRGFDQDMANK 235
             ++SD   SLP+ P +  +VG+ Y+ LE       +G +V VMV +   LL G D+++A K
Sbjct: 41809 MTSDTCVSLPWLPSEICIVGSGYIGLEFMDVFTSLGSEV-VMVEAGPRLLPGVDKEVA-K 41636

Query: 236   IGEHM-----EEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSE 277
             + E +     +E  +K     +  +V  +  G  G   V  Q T+++
Sbjct: 41635 LAERLLLQQFKERPVKLYTNTLASQVRPL--GPKGEAPVEVQLTDAQ 41501



 Score = 33.5 bits (75), Expect = 0.77
 Identities = 18/45 (40%), Positives = 26/45 (57%), Gaps = 3/45 (6%)
 Frame = -2

Query: 56    GGTCVNVGCIPKKLMHQAALLGQALQDSRN---YGWKVEETVKHD 97
             GGTCVN GC+P K +  AA   + L++  +    G +VE  +K D
Sbjct: 43020 GGTCVNRGCVPSKALLAAARRVKMLRNKHHLSAMGLQVEGEIKVD 42886


>gb|AAQM02000202.1| Toxoplasma gondii GT1 gcontig_1107000988482, whole genome shotgun
             sequence
          Length = 81957

 Score = 34.3 bits (77), Expect = 0.45
 Identities = 30/122 (24%), Positives = 48/122 (39%), Gaps = 1/122 (0%)
 Frame = +1

Query: 69    LMHQAALLGQALQDSRNYGWKVEETVKHDW-DRMIEAVQNHIGSLNWGYRVALREKKVVY 127
             L   + LL    Q     GW  EE    D  + +  A     GS+NW  ++ L++ +V  
Sbjct: 69541 LSAHSLLLAMGRQSVTKQGWMSEEINVQDMCNELTAATSKSPGSVNWRSKIRLQQNQVAA 69720

Query: 128   ENAYGQFIGPHRIKATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLP 187
                         + A   KG +  Y         G+     G PGD ++C+++  LF+L 
Sbjct: 69721 V----------AVSAECLKGDQDKYWL-------GD-----GAPGDGDWCLAAQRLFALA 69834

Query: 188   YC 189
              C
Sbjct: 69835 AC 69840


>gb|AAQM02000042.1| Toxoplasma gondii GT1 gcontig_1107000930636, whole genome shotgun
              sequence
          Length = 384686

 Score = 32.7 bits (73), Expect = 1.3
 Identities = 13/17 (76%), Positives = 14/17 (82%)
 Frame = +3

Query: 52     RWGLGGTCVNVGCIPKK 68
              R  LGGTC+NVGCIP K
Sbjct: 233922 RGTLGGTCLNVGCIPSK 233972


>gb|AAQM02000054.1| Toxoplasma gondii GT1 gcontig_1107000855630, whole genome shotgun
              sequence
          Length = 335650

 Score = 30.0 bits (66), Expect = 8.6
 Identities = 17/57 (29%), Positives = 25/57 (43%)
 Frame = -1

Query: 227    GFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGE 283
              G D +++ + GEH E        +   ++ EQ  AG  G    VAQ+     II  E
Sbjct: 118117 GPDDELSGQSGEHAEAKCSTIAAELQQLRAEQANAGRVGTANFVAQAKLQRRIIRLE 117947


>gb|AAQM02000024.1| Toxoplasma gondii GT1 gcontig_1107000833414, whole genome shotgun
              sequence
          Length = 472200

 Score = 30.0 bits (66), Expect = 8.6
 Identities = 16/48 (33%), Positives = 21/48 (43%)
 Frame = -1

Query: 164    RPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAG 211
              R   L IP  +E C   D+LFSL    G T +   + V +    F  G
Sbjct: 147018 RENELHIPATREVCFKEDELFSLEVSVGHTSINLDAQVGMSSPSFSGG 146875


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 66,210,174
  Number of sequences in database:  3590
  
Lambda     K      H
   0.319    0.138    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,635,735
Number of Sequences: 3590
Number of extensions: 554052
Number of successful extensions: 2386
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 57
Number of HSP's that attempted gapping in prelim test: 1775
Number of HSP's gapped (non-prelim): 1157
length of query: 499
length of database: 22,070,058
effective HSP length: 109
effective length of query: 390
effective length of database: 21,678,748
effective search space: 8454711720
effective search space used: 8454711720
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 66 (30.0 bits)
