TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000001_1.0 # Protein # Iodothyronine deiodinase 1 (DI1) #
Homo sapiens # Complete
         (249 letters)

Database: genome.fa 
           7809 sequences; 167,727,055 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_253                                                           57   6e-08
scaffold_164                                                           57   6e-08
scaffold_7                                                             45   2e-04
scaffold_494                                                           45   3e-04
scaffold_1614                                                          44   4e-04
scaffold_405                                                           44   4e-04
scaffold_74                                                            33   0.92 
scaffold_101                                                           33   1.2  
scaffold_13                                                            31   4.6  
scaffold_414                                                           30   6.0  
scaffold_653                                                           30   7.8  

>scaffold_253 
          Length = 194081

 Score = 57.0 bits (136), Expect = 6e-08
 Identities = 55/181 (30%), Positives = 84/181 (46%), Gaps = 15/181 (8%)
 Frame = -1

Query: 80   QRLEDTTELGGLAPNCPVVRLSGQRCNIWEFMQ---GNRPL-VLNFGSCTXPSFMFKFDQ 135
            Q  + +  LG LAP+ PV  L G    +   ++   G R L VLNFGS T P ++ K   
Sbjct: 9767 QGTKPSLTLGSLAPDGPVCTLDGVETTLHGAVRAGSGPRKLTVLNFGSYT*PPYVSKGPA 9588

Query: 136  F---KRLIEDFSSIADFLVIYIEEAHASDGWAF--KNNMDIRNHQNLQDRLQAAHLLLAR 190
                 R   D  S++ FLV YI EAH   GWA    + +  R  + L  RL  A      
Sbjct: 9587 LVTSMRPYLDAGSLSLFLV-YIREAHPEGGWAIPAADGICYRQPKTLASRLAVAAKFAEH 9411

Query: 191  SPQ----CPVVVDTMQNQSSQL-YAALPERLYIIQEG-RILYKGKSGPWNYNPEEVRAVL 244
              +     P++VD     +  + Y A PERL ++ E   +++    GP+ Y+  ++ A +
Sbjct: 9410 QAEWCAGVPMLVDDPTTNALDIAYEAPPERLVVLDEQLNVVFFSGQGPFQYSVPKLAAFV 9231

Query: 245  E 245
            +
Sbjct: 9230 K 9228


>scaffold_164 
          Length = 300493

 Score = 57.0 bits (136), Expect = 6e-08
 Identities = 55/181 (30%), Positives = 84/181 (46%), Gaps = 15/181 (8%)
 Frame = +3

Query: 80     QRLEDTTELGGLAPNCPVVRLSGQRCNIWEFMQ---GNRPL-VLNFGSCTXPSFMFKFDQ 135
              Q  + +  LG LAP+ PV  L G    +   ++   G R L VLNFGS T P ++ K   
Sbjct: 288555 QGTKPSLTLGSLAPDGPVCTLDGVETTLHGAVRAGSGPRKLTVLNFGSYT*PPYVSKGPA 288734

Query: 136    F---KRLIEDFSSIADFLVIYIEEAHASDGWAF--KNNMDIRNHQNLQDRLQAAHLLLAR 190
                   R   D  S++ FLV YI EAH   GWA    + +  R  + L  RL  A      
Sbjct: 288735 LVTSMRPYLDAGSLSLFLV-YIREAHPEGGWAIPAADGICYRQPKTLASRLAVAAKFAEH 288911

Query: 191    SPQ----CPVVVDTMQNQSSQL-YAALPERLYIIQEG-RILYKGKSGPWNYNPEEVRAVL 244
                +     P++VD     +  + Y A PERL ++ E   +++    GP+ Y+  ++ A +
Sbjct: 288912 QAEWCAGVPMLVDDPTTNALDIAYEAPPERLVVLDEQLNVVFFSGQGPFQYSVPKLAAFV 289091

Query: 245    E 245
              +
Sbjct: 289092 K 289094


>scaffold_7 
          Length = 1460757

 Score = 45.4 bits (106), Expect = 2e-04
 Identities = 44/164 (26%), Positives = 68/164 (41%), Gaps = 16/164 (9%)
 Frame = +2

Query: 75     LKVRWQRLE-DTTELGGLAPNCPVVRL------SGQRCNIWEFMQGNRPLVLNFGSCTXP 127
              L+VR  R E  T   G   P+ P+  L      +     +     G  P +L  GS T P
Sbjct: 270281 LQVRHNRAERGTLRDGDALPDVPLFPLRPGAAAAAAAVGLRAACAGPAPTLLVAGSFT*P 270460

Query: 128    SFMFKFDQFKRLIEDFSSIADFLVIYIEEAHASDGWAFKNNM------DIRNHQNLQDRL 181
                         L  +F+  A   ++YI EAHA+D W   ++        +    +L +R 
Sbjct: 270461 PLRGLAPSLASLSREFAGRARVQLVYIAEAHAADEWPINSSRCAGPANSVLRPTSLAERR 270640

Query: 182    QAAHLLLARSP---QCPVVVDTMQNQSSQLYAALPERLYIIQEG 222
                A  +LA  P     P++VD + +   +  AA P RLY ++ G
Sbjct: 270641 AVAERMLAALPCLAPLPLLVDGLDDALLRALAAWPIRLYGVRGG 270772


>scaffold_494 
          Length = 46337

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 34/119 (28%), Positives = 53/119 (44%), Gaps = 9/119 (7%)
 Frame = +3

Query: 113   GNRPLVLNFGSCTXPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHASDGWAFKNNM--- 169
             G  P +L  GS T P           L  +F+  A   ++YI EAHA+D W   ++    
Sbjct: 11829 GPAPTLLVAGSFT*PPLRGLAPSLASLSREFAGRARVQLVYIAEAHAADEWPINSSRCAG 12008

Query: 170   ---DIRNHQNLQDRLQAAHLLLARSP---QCPVVVDTMQNQSSQLYAALPERLYIIQEG 222
                 +    +L +R   A  +LA  P     P++VD + +   +  AA P RLY ++ G
Sbjct: 12009 PANSVLRPTSLAERRAVAERMLAALPCLAPLPLLVDGLDDALLRALAAWPIRLYGVRGG 12185


>scaffold_1614 
          Length = 7841

 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 40/147 (27%), Positives = 64/147 (43%), Gaps = 4/147 (2%)
 Frame = +2

Query: 77  VRWQRLEDTTELGGLA-PNCPVVRLS-GQRCNIWEFMQGNRPLVLNFGSCTXPSFMFKFD 134
           VR+ R    T   G A P+  + RLS G   ++ + +    P  L   S T P F     
Sbjct: 449 VRFNRCRSGTLSSGCALPDVILARLSDGGAVSLLDSLPAAGPTFLLAASFT*PPFRAHAQ 628

Query: 135 QFKRLIEDFSSIADFLVIYIEEAHASDGWAFKNNMDIRNHQNLQDRLQAAHLLLAR-SPQ 193
             ++L      +  F ++Y  EAHASD W   +++  R  ++  +R+  A   LA     
Sbjct: 629 LLEQLRVRADGVVRFQLVYTAEAHASDEWPVGSHIAQRQPRSTHERVSVARRRLAELGVN 808

Query: 194 CP-VVVDTMQNQSSQLYAALPERLYII 219
            P  +VDT +    + +A  P R Y +
Sbjct: 809 WPGTLVDTGREAFQEAFACWPLRWYFL 889


>scaffold_405 
          Length = 74758

 Score = 44.3 bits (103), Expect = 4e-04
 Identities = 40/147 (27%), Positives = 64/147 (43%), Gaps = 4/147 (2%)
 Frame = +2

Query: 77    VRWQRLEDTTELGGLA-PNCPVVRLS-GQRCNIWEFMQGNRPLVLNFGSCTXPSFMFKFD 134
             VR+ R    T   G A P+  + RLS G   ++ + +    P  L   S T P F     
Sbjct: 52082 VRFNRCRSGTLSSGCALPDVILARLSDGGAVSLLDSLPAAGPTFLLAASFT*PPFRAHAQ 52261

Query: 135   QFKRLIEDFSSIADFLVIYIEEAHASDGWAFKNNMDIRNHQNLQDRLQAAHLLLAR-SPQ 193
               ++L      +  F ++Y  EAHASD W   +++  R  ++  +R+  A   LA     
Sbjct: 52262 LLEQLRVRADGVVRFQLVYTAEAHASDEWPVGSHIAQRQPRSTHERVSVARRRLAELGVN 52441

Query: 194   CP-VVVDTMQNQSSQLYAALPERLYII 219
              P  +VDT +    + +A  P R Y +
Sbjct: 52442 WPGTLVDTGREAFQEAFACWPLRWYFL 52522


>scaffold_74 
          Length = 565035

 Score = 33.1 bits (74), Expect = 0.92
 Identities = 16/43 (37%), Positives = 23/43 (53%)
 Frame = +1

Query: 88     LGGLAPNCPVVRLSGQRCNIWEFMQGNRPLVLNFGSCTXPSFM 130
              +GG AP+    RL G    +   +   RP +L+FGS T P F+
Sbjct: 430417 VGGPAPDGACHRLDGSASTLLAELHAERPTILSFGSAT*PPFV 430545


>scaffold_101 
          Length = 510310

 Score = 32.7 bits (73), Expect = 1.2
 Identities = 17/39 (43%), Positives = 20/39 (51%), Gaps = 4/39 (10%)
 Frame = +1

Query: 72     WFVLKVRWQRLEDTT----ELGGLAPNCPVVRLSGQRCN 106
              WF  K  W+RL  T       GG AP  P  R SG+RC+
Sbjct: 238987 WFARKGTWRRLATTALRPRSGGGHAPTLPTWRRSGRRCS 239103


>scaffold_13 
          Length = 1180512

 Score = 30.8 bits (68), Expect = 4.6
 Identities = 25/96 (26%), Positives = 37/96 (38%), Gaps = 5/96 (5%)
 Frame = +2

Query: 123    SCTXPSFMFKFDQFKRLIEDFSSI-----ADFLVIYIEEAHASDGWAFKNNMDIRNHQNL 177
              SC     M   D+F  L+ED   +     +  L +  E   A DGWA + NM++      
Sbjct: 504467 SCILRPLMAASDEFGCLLEDKDDLHSRRLSGALSVAHEAFIAIDGWALRGNMEV------ 504628

Query: 178    QDRLQAAHLLLARSPQCPVVVDTMQNQSSQLYAALP 213
                 L   H    R P CP   D      ++ ++  P
Sbjct: 504629 ---LPWVH----RGPSCPCEQDVTAGGEAKAWSETP 504715


>scaffold_414 
          Length = 79634

 Score = 30.4 bits (67), Expect = 6.0
 Identities = 14/31 (45%), Positives = 18/31 (58%), Gaps = 5/31 (16%)
 Frame = -2

Query: 36    RVKRNILAMGEKTGMTRNP-----HFSHDNW 61
             RVKR  +AMG +TG+ R P     HF+   W
Sbjct: 63304 RVKRRFVAMGRRTGVVRWPSAPLRHFAQSEW 63212


>scaffold_653 
          Length = 24147

 Score = 30.0 bits (66), Expect = 7.8
 Identities = 12/32 (37%), Positives = 17/32 (53%)
 Frame = -2

Query: 60    NWIPTFFSTQYFWFVLKVRWQRLEDTTELGGL 91
             +W+   +S+Q  WF+    WQ  E  T LG L
Sbjct: 17138 SWVGAHYSSQRSWFLCTRSWQSSE*ATRLGAL 17043


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 167,727,055
  Number of sequences in database:  7809
  
Lambda     K      H
   0.324    0.138    0.448 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,552,370
Number of Sequences: 7809
Number of extensions: 748765
Number of successful extensions: 3908
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 432
Number of HSP's successfully gapped in prelim test: 80
Number of HSP's that attempted gapping in prelim test: 2535
Number of HSP's gapped (non-prelim): 2155
length of query: 249
length of database: 55,909,018
effective HSP length: 109
effective length of query: 140
effective length of database: 55,057,837
effective search space: 7708097180
effective search space used: 7708097180
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 65 (29.6 bits)