TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SelTryp; hypothetical protein [Trypanosoma brucei TREU927]
PARTIALLY FROM XP_844760.1
         (259 letters)

Database: genome.fa 
           218 sequences; 41,633,360 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_10                                                            32   0.45 
scaffold_7                                                             29   3.8  
scaffold_4                                                             28   8.5  

>scaffold_10 
          Length = 1258938

 Score = 32.3 bits (72), Expect = 0.45
 Identities = 15/23 (65%), Positives = 17/23 (73%)
 Frame = -3

Query: 213    SGAFEVYLNGSLIYSKLETGAVP 235
              SGAFEV +NG LI+SK E G  P
Sbjct: 782818 SGAFEVVVNGKLIHSKKERGNFP 782750


>scaffold_7 
          Length = 1435906

 Score = 29.3 bits (64), Expect = 3.8
 Identities = 12/23 (52%), Positives = 19/23 (82%)
 Frame = +2

Query: 201    FMMNMVASSLLQSGAFEVYLNGS 223
              F+ + VA +LL +GAFE+Y++GS
Sbjct: 784277 FVGSTVAQNLLTTGAFEIYVDGS 784345



 Score = 28.9 bits (63), Expect = 5.0
 Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
 Frame = +1

Query: 127    PELREQQKGKKPFIKFVGVVYSVGAFRE--FIGNILSTGFLASIAISFFAPFLRGALPPH 184
              P LR+  +  KPF   +G ++ VG+FR   FI +      +    + FF P LR     H
Sbjct: 469147 PRLRQHHE-TKPFQPLIGSLFVVGSFRAAWFINSARCQAGVGYWNLQFFIPCLRNVKHRH 469323


>scaffold_4 
          Length = 2081438

 Score = 28.1 bits (61), Expect = 8.5
 Identities = 20/57 (35%), Positives = 34/57 (59%), Gaps = 9/57 (15%)
 Frame = +3

Query: 45     LEMLKEA-------DLKQMLHEKGEAFHHLRSKRELILAVLKLEK--REEALRKARV 92
              LE+ +EA       DLK+ + + G ++  +  K+ELI A+LK +K   +EAL + +V
Sbjct: 315732 LELTQEALSAVSVKDLKKEMQKYGISYAGMSEKQELIQALLKHKK*SPQEALARPKV 315902


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:06 PM
  Number of letters in database: 41,633,360
  Number of sequences in database:  218
  
Lambda     K      H
   0.321    0.136    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,712,907
Number of Sequences: 218
Number of extensions: 127245
Number of successful extensions: 604
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 518
Number of HSP's gapped (non-prelim): 212
length of query: 259
length of database: 13,877,786
effective HSP length: 100
effective length of query: 159
effective length of database: 13,855,986
effective search space: 2203101774
effective search space used: 2203101774
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)